Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              19     869
XP_006717989                                                           19     869
NP_001167554                                                           16    5631
NP_001167553                                                           16    5631
NP_060322                                                              16    5631
NP_001167552                                                           16    5631
NP_005325                                                              15    9605
NP_940905                                                              15    9605
NP_001274748                                                           15    9605
NP_001274749                                                           15    9605
XP_005274721                                                           15    9605
XP_006724879                                                           15    9605
XP_006724878                                                           15    9605
XP_006711358                                                           15    9605
XP_006711357                                                           15    9605
>NP_000305
          Length = 403

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMMFETILM 9
           KMMFETI M
Sbjct: 197 KMMFETIPM 205
>XP_006717989
          Length = 388

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMMFETILM 9
           KMMFETI M
Sbjct: 182 KMMFETIPM 190
>NP_001167554
          Length = 1039

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMMFETI 7
           KM+FET+
Sbjct: 977 KMLFETL 983
>NP_001167553
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMMFETI 7
           KM+FET+
Sbjct: 978 KMLFETL 984
>NP_060322
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMMFETI 7
            KM+FET+
Sbjct: 1000 KMLFETL 1006
>NP_001167552
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMMFETI 7
            KM+FET+
Sbjct: 1000 KMLFETL 1006
>NP_005325
          Length = 2035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MMFETILM 9
           M +ETILM
Sbjct: 304 MAWETILM 311
>NP_940905
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MFETILM 9
           +FETIL+
Sbjct: 116 LFETILL 122
>NP_001274748
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MFETILM 9
           +FETIL+
Sbjct: 116 LFETILL 122
>NP_001274749
          Length = 1068

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MFETILM 9
           +FETIL+
Sbjct: 116 LFETILL 122
>XP_005274721
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MMFETILM 9
           M +ETILM
Sbjct: 304 MAWETILM 311
>XP_006724879
          Length = 2079

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MMFETILM 9
           M +ETILM
Sbjct: 304 MAWETILM 311
>XP_006724878
          Length = 2080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MMFETILM 9
           M +ETILM
Sbjct: 304 MAWETILM 311
>XP_006711358
          Length = 1118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MFETILM 9
           +FETIL+
Sbjct: 116 LFETILL 122
>XP_006711357
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MFETILM 9
           +FETIL+
Sbjct: 116 LFETILL 122
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.144    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 276,271
Number of extensions: 304
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package