Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001155197                                                           17    4312
NP_848702                                                              17    4312
NP_001155198                                                           17    4312
NP_848701                                                              17    4312
NP_002710                                                              17    4312
NP_001257405                                                           17    4312
NP_006236                                                              17    4312
NP_851308                                                              17    4312
NP_851307                                                              17    4312
XP_005267884                                                           17    4312
XP_005267883                                                           17    4312
XP_005267881                                                           17    4312
XP_005267880                                                           17    4312
XP_005267879                                                           17    4312
XP_005267877                                                           17    4312
XP_005267876                                                           17    4312
NP_443732                                                              17    4312
NP_001068                                                              16    5631
NP_001073990                                                           16    7355
NP_005438                                                              16    7355
NP_001299                                                              15    9605
XP_006714215                                                           15    9605
XP_006714214                                                           15    9605
XP_005262915                                                           15    9605
NP_055806                                                              15    9605
>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 132 KMFCQLAKT 140
>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 93  KMFCQLAKT 101
>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 132 KMFCQLAKT 140
>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 132 KMFCQLAKT 140
>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 93  KMFCQLAKT 101
>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 93  KMFCQLAKT 101
>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 93  KMFCQLAKT 101
>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 132 KMFCQLAKT 140
>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMFRQLAKT 9
           KMF QLAKT
Sbjct: 93  KMFCQLAKT 101
>NP_001155197
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 358 LFRQLAK 364
>NP_848702
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 327 LFRQLAK 333
>NP_001155198
          Length = 540

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 382 LFRQLAK 388
>NP_848701
          Length = 485

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 327 LFRQLAK 333
>NP_002710
          Length = 524

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 327 LFRQLAK 333
>NP_001257405
          Length = 451

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 252 LFRQLAK 258
>NP_006236
          Length = 602

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 403 LFRQLAK 409
>NP_851308
          Length = 496

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 297 LFRQLAK 303
>NP_851307
          Length = 570

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 371 LFRQLAK 377
>XP_005267884
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 252 LFRQLAK 258
>XP_005267883
          Length = 500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 303 LFRQLAK 309
>XP_005267881
          Length = 539

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 387 LFRQLAK 393
>XP_005267880
          Length = 545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 387 LFRQLAK 393
>XP_005267879
          Length = 556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 387 LFRQLAK 393
>XP_005267877
          Length = 579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 382 LFRQLAK 388
>XP_005267876
          Length = 584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MFRQLAK 8
           +FRQLAK
Sbjct: 387 LFRQLAK 393
>NP_443732
          Length = 358

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KMFRQLA 7
           KMFRQL+
Sbjct: 115 KMFRQLS 121
>NP_001068
          Length = 530

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FRQLAKT 9
           FR+LAKT
Sbjct: 517 FRKLAKT 523
>NP_001073990
          Length = 507

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KMFRQLAK 8
           K FR+LAK
Sbjct: 179 KAFRKLAK 186
>NP_005438
          Length = 435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KMFRQLAK 8
           K FR+LAK
Sbjct: 163 KTFRKLAK 170
>NP_001299
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KMFRQLAK 8
           K+F++LAK
Sbjct: 249 KLFQKLAK 256
>XP_006714215
          Length = 3544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KMFRQLAK 8
            K FR LAK
Sbjct: 2765 KTFRNLAK 2772
>XP_006714214
          Length = 3544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KMFRQLAK 8
            K FR LAK
Sbjct: 2765 KTFRNLAK 2772
>XP_005262915
          Length = 3544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KMFRQLAK 8
            K FR LAK
Sbjct: 2765 KTFRNLAK 2772
>NP_055806
          Length = 3526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KMFRQLAK 8
            K FR LAK
Sbjct: 2747 KTFRNLAK 2754
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.132    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 292,075
Number of extensions: 393
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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