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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     299
NP_000042                                                              16    7355
NP_065099                                                              16    7355
NP_573400                                                              16    7355
NP_008981                                                              16    7355
NP_005659                                                              16    7355
XP_006723752                                                           16    7355
XP_006723751                                                           16    7355
XP_006723750                                                           16    7355
XP_006723749                                                           16    7355
XP_006723748                                                           16    7355
XP_006720273                                                           16    7355
XP_006720272                                                           16    7355
XP_005267938                                                           16    7355
XP_005267937                                                           16    7355
XP_006720271                                                           16    7355
XP_005267936                                                           16    7355
XP_005271621                                                           16    7355
XP_006718907                                                           16    7355
XP_006718906                                                           16    7355
XP_005271619                                                           16    7355
XP_005271618                                                           16    7355
NP_005821                                                              15    9605
NP_001243275                                                           15    9605
NP_005012                                                              15    9605
NP_001026872                                                           15    9605
NP_001243277                                                           15    9605
NP_001243276                                                           15    9605

>NP_002430
          Length = 1137

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KMEFMTIN 8
           KMEFMTIN
Sbjct: 531 KMEFMTIN 538



>NP_000042
          Length = 3056

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMEFMTI 7
           KM+F+TI
Sbjct: 659 KMDFLTI 665



>NP_065099
          Length = 1286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1    KMEFMTINV 9
            +ME++++N+
Sbjct: 1018 RMEYLSLNI 1026



>NP_573400
          Length = 1460

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1195 KDEFQTLNI 1203



>NP_008981
          Length = 1441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1176 KDEFQTLNI 1184



>NP_005659
          Length = 359

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MEFMTINV 9
           +EF T+NV
Sbjct: 335 LEFKTLNV 342



>XP_006723752
          Length = 1387

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1122 KDEFQTLNI 1130



>XP_006723751
          Length = 1427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1162 KDEFQTLNI 1170



>XP_006723750
          Length = 1427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1162 KDEFQTLNI 1170



>XP_006723749
          Length = 1428

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1163 KDEFQTLNI 1171



>XP_006723748
          Length = 1437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KMEFMTINV 9
            K EF T+N+
Sbjct: 1172 KDEFQTLNI 1180



>XP_006720273
          Length = 508

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   KMEFMTINV 9
           +ME++++N+
Sbjct: 239 RMEYLSLNI 247



>XP_006720272
          Length = 612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   KMEFMTINV 9
           +ME++++N+
Sbjct: 344 RMEYLSLNI 352



>XP_005267938
          Length = 745

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   KMEFMTINV 9
           +ME++++N+
Sbjct: 476 RMEYLSLNI 484



>XP_005267937
          Length = 918

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   KMEFMTINV 9
           +ME++++N+
Sbjct: 649 RMEYLSLNI 657



>XP_006720271
          Length = 928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   KMEFMTINV 9
           +ME++++N+
Sbjct: 660 RMEYLSLNI 668



>XP_005267936
          Length = 929

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   KMEFMTINV 9
           +ME++++N+
Sbjct: 660 RMEYLSLNI 668



>XP_005271621
          Length = 2708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMEFMTI 7
           KM+F+TI
Sbjct: 311 KMDFLTI 317



>XP_006718907
          Length = 3050

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMEFMTI 7
           KM+F+TI
Sbjct: 659 KMDFLTI 665



>XP_006718906
          Length = 3056

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMEFMTI 7
           KM+F+TI
Sbjct: 659 KMDFLTI 665



>XP_005271619
          Length = 3056

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMEFMTI 7
           KM+F+TI
Sbjct: 659 KMDFLTI 665



>XP_005271618
          Length = 3056

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KMEFMTI 7
           KM+F+TI
Sbjct: 659 KMDFLTI 665



>NP_005821
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KMEFMTINV 9
           K+E  T+NV
Sbjct: 107 KVELSTVNV 115



>NP_001243275
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MEFMTIN 8
            MEF T+N
Sbjct: 1096 MEFKTLN 1102



>NP_005012
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KMEFMT 6
           KME+MT
Sbjct: 382 KMEYMT 387



>NP_001026872
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MEFMTIN 8
            MEF T+N
Sbjct: 1096 MEFKTLN 1102



>NP_001243277
          Length = 1057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MEFMTIN 8
            MEF T+N
Sbjct: 1002 MEFKTLN 1008



>NP_001243276
          Length = 1205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    MEFMTIN 8
            MEF T+N
Sbjct: 1150 MEFKTLN 1156



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.136    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 295,380
Number of extensions: 392
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)