Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              23      60
NP_001075109                                                           23      60
NP_620595                                                              18    1935
NP_620594                                                              18    1935
NP_620596                                                              18    1935
XP_005272075                                                           18    1935
NP_057688                                                              18    1935
NP_001561                                                              17    3301
NP_001128577                                                           17    3301
NP_060617                                                              16    5631
NP_872358                                                              16    5631
NP_001189479                                                           16    5631
NP_689961                                                              16    5631
NP_001136221                                                           16    5631
NP_001028204                                                           16    5631
NP_001028203                                                           16    5631
NP_001028202                                                           16    5631
XP_006715958                                                           16    5631
XP_006715957                                                           16    5631
NP_073739                                                              16    7355
NP_001139150                                                           16    7355
NP_116202                                                              16    7355
NP_775936                                                              16    7355
NP_112600                                                              16    7355
NP_001073137                                                           16    7355
NP_001273382                                                           16    7355
XP_006720018                                                           16    7355
XP_005254139                                                           16    7355
XP_006720017                                                           16    7355
XP_005272109                                                           16    7355
XP_006714651                                                           16    7355
NP_004035                                                              15    9605
NP_689604                                                              15    9605
NP_689600                                                              15    9605
NP_036587                                                              15    9605
NP_612490                                                              15    9605
NP_001269639                                                           15    9605
NP_005081                                                              15    9605
NP_001185517                                                           15    9605
NP_001158058                                                           15    9605
XP_005261428                                                           15    9605
XP_005261427                                                           15    9605
XP_005250136                                                           15    9605
XP_006715991                                                           15    9605
XP_006715990                                                           15    9605
XP_006712608                                                           15    9605
XP_006712455                                                           15    9605
XP_005263691                                                           15    9605
XP_005246358                                                           15    9605
XP_006711681                                                           15    9605
XP_006711680                                                           15    9605
XP_005245637                                                           15    9605
XP_006710258                                                           15    9605
NP_775949                                                              15    9605
NP_001123497                                                           15    9605
NP_001108104                                                           15    9605
>NP_008835
          Length = 4128

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KMDAMNIWD 9
            KMD MNIWD
Sbjct: 3172 KMDPMNIWD 3180
>NP_001075109
          Length = 4097

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KMDAMNIWD 9
            KMD MNIWD
Sbjct: 3172 KMDPMNIWD 3180
>NP_620595
          Length = 1340

 Score = 17.7 bits (34), Expect =  1935
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KMDAMNIWD 9
           +MD+  +WD
Sbjct: 504 RMDSQQVWD 512
>NP_620594
          Length = 1427

 Score = 17.7 bits (34), Expect =  1935
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KMDAMNIWD 9
           +MD+  +WD
Sbjct: 535 RMDSQQVWD 543
>NP_620596
          Length = 1371

 Score = 17.7 bits (34), Expect =  1935
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   KMDAMNIWD 9
           +MD+  +WD
Sbjct: 535 RMDSQQVWD 543
>XP_005272075
          Length = 1561

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   DAMNIW 8
           DAMN+W
Sbjct: 767 DAMNLW 772
>NP_057688
          Length = 1761

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   DAMNIW 8
           DAMN+W
Sbjct: 967 DAMNLW 972
>NP_001561
          Length = 625

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MDAMNIWD 9
          MDA++ WD
Sbjct: 20 MDALSEWD 27
>NP_001128577
          Length = 322

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KMDAMNIW 8
           K+ +MN+W
Sbjct: 257 KVSSMNVW 264
>NP_060617
          Length = 179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   DAMNIW 8
           DAM++W
Sbjct: 116 DAMDVW 121
>NP_872358
          Length = 895

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 5/7 (71%)

Query: 3   DAMNIWD 9
           D M +WD
Sbjct: 763 DGMRLWD 769
>NP_001189479
          Length = 730

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 5/7 (71%)

Query: 3   DAMNIWD 9
           D M +WD
Sbjct: 636 DGMRLWD 642
>NP_689961
          Length = 862

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KMDAMNIWD 9
           K D MN W+
Sbjct: 294 KCDGMNGWE 302
>NP_001136221
          Length = 1029

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KMDAMNIWD 9
           K D MN W+
Sbjct: 461 KCDGMNGWE 469
>NP_001028204
          Length = 179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   DAMNIW 8
           DAM++W
Sbjct: 116 DAMDVW 121
>NP_001028203
          Length = 201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   DAMNIW 8
           DAM++W
Sbjct: 138 DAMDVW 143
>NP_001028202
          Length = 213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 3   DAMNIW 8
           DAM++W
Sbjct: 150 DAMDVW 155
>XP_006715958
          Length = 915

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KMDAMNIWD 9
           K D MN W+
Sbjct: 347 KCDGMNGWE 355
>XP_006715957
          Length = 915

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KMDAMNIWD 9
           K D MN W+
Sbjct: 347 KCDGMNGWE 355
>NP_073739
          Length = 1430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MDAMNIWD 9
           +DAM+ W+
Sbjct: 816 IDAMDTWE 823
>NP_001139150
          Length = 1275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           MD  ++WD
Sbjct: 155 MDVKSMWD 162
>NP_116202
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           +DA+N W
Sbjct: 620 VDALNAW 626
>NP_775936
          Length = 324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           MD  ++WD
Sbjct: 155 MDVKSMWD 162
>NP_112600
          Length = 442

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   KMDAMNIW 8
           +MD  N+W
Sbjct: 234 EMDLRNLW 241
>NP_001073137
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           +DA+N W
Sbjct: 601 VDALNAW 607
>NP_001273382
          Length = 630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           +DA+N W
Sbjct: 490 VDALNAW 496
>XP_006720018
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           +DA+N W
Sbjct: 374 VDALNAW 380
>XP_005254139
          Length = 524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           +DA+N W
Sbjct: 384 VDALNAW 390
>XP_006720017
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           +DA+N W
Sbjct: 601 VDALNAW 607
>XP_005272109
          Length = 1123

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   MDAMNIWD 9
           +DAM+ W+
Sbjct: 816 IDAMDTWE 823
>XP_006714651
          Length = 1280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           MD  ++WD
Sbjct: 155 MDVKSMWD 162
>NP_004035
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 3   DAMNIW 8
           DA+N W
Sbjct: 242 DALNAW 247
>NP_689604
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           M A+NIW
Sbjct: 180 MLALNIW 186
>NP_689600
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           MDA + W+
Sbjct: 211 MDAFDAWE 218
>NP_036587
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   DAMNIWD 9
           DA+++W+
Sbjct: 991 DALDLWE 997
>NP_612490
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           +DA+  WD
Sbjct: 347 LDALRPWD 354
>NP_001269639
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   MDAMNIW 8
           M A+NIW
Sbjct: 166 MLALNIW 172
>NP_005081
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 3   DAMNIW 8
           DA+N W
Sbjct: 162 DAVNFW 167
>NP_001185517
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 3   DAMNIW 8
           DA+N W
Sbjct: 162 DAVNFW 167
>NP_001158058
          Length = 3708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   KMDAMNIW 8
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_005261428
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           +DA+  WD
Sbjct: 347 LDALRPWD 354
>XP_005261427
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           +DA+  WD
Sbjct: 347 LDALRPWD 354
>XP_005250136
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2  MDAMNIWD 9
          MD +  WD
Sbjct: 13 MDGVTCWD 20
>XP_006715991
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2  MDAMNIWD 9
          MD +  WD
Sbjct: 13 MDGVTCWD 20
>XP_006715990
          Length = 919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2  MDAMNIWD 9
          MD +  WD
Sbjct: 13 MDGVTCWD 20
>XP_006712608
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 3   DAMNIW 8
           DA+N W
Sbjct: 183 DALNAW 188
>XP_006712455
          Length = 1055

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   DAMNIWD 9
           DA+++W+
Sbjct: 990 DALDLWE 996
>XP_005263691
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 3   DAMNIWD 9
           DA+++W+
Sbjct: 991 DALDLWE 997
>XP_005246358
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MDAMNIWD 9
           MDA + W+
Sbjct: 211 MDAFDAWE 218
>XP_006711681
          Length = 3448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   KMDAMNIW 8
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_006711680
          Length = 3617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   KMDAMNIW 8
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_005245637
          Length = 3683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1   KMDAMNIW 8
           KMD   IW
Sbjct: 161 KMDLEQIW 168
>XP_006710258
          Length = 216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2  MDAMNIWD 9
          MD +  WD
Sbjct: 13 MDGVTCWD 20
>NP_775949
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2  MDAMNIWD 9
          MD +  WD
Sbjct: 13 MDGVTCWD 20
>NP_001123497
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2  MDAMNIWD 9
          MD +  WD
Sbjct: 13 MDGVTCWD 20
>NP_001108104
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 3   DAMNIW 8
           DA+N W
Sbjct: 162 DAVNFW 167
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.130    0.474 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 515,898
Number of extensions: 839
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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