Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              20     509
NP_001091680                                                           20     509
NP_001091679                                                           20     509
XP_006713048                                                           20     509
XP_006713047                                                           20     509
XP_006713046                                                           20     509
XP_005264943                                                           20     509
NP_001186091                                                           17    4312
NP_001186090                                                           17    4312
NP_006668                                                              17    4312
NP_001186089                                                           17    4312
XP_005264888                                                           17    4312
XP_006713016                                                           17    4312
XP_005264887                                                           17    4312
XP_006713015                                                           17    4312
XP_006713014                                                           17    4312
XP_006713013                                                           17    4312
XP_006713012                                                           17    4312
XP_006713011                                                           17    4312
XP_005264886                                                           17    4312
XP_006713010                                                           17    4312
XP_005264885                                                           17    4312
XP_005264884                                                           17    4312
XP_005264883                                                           17    4312
XP_005264882                                                           17    4312
XP_006713009                                                           17    4312
XP_005264881                                                           17    4312
XP_005264880                                                           17    4312
NP_001518                                                              16    5631
NP_001011705                                                           16    5631
NP_008946                                                              16    5631
NP_002806                                                              16    5631
NP_001257411                                                           16    5631
XP_006719824                                                           16    5631
XP_006719823                                                           16    5631
NP_653272                                                              16    7355
NP_001171468                                                           16    7355
NP_001171467                                                           16    7355
XP_006719320                                                           16    7355
NP_006438                                                              15    9605
NP_001001992                                                           15    9605
NP_821133                                                              15    9605
NP_001027582                                                           15    9605
NP_116253                                                              15    9605
NP_060546                                                              15    9605
NP_001276949                                                           15    9605
NP_001276950                                                           15    9605
NP_001274427                                                           15    9605
NP_115671                                                              15    9605
NP_996996                                                              15    9605
NP_872433                                                              15    9605
XP_005252542                                                           15    9605
XP_006717518                                                           15    9605
XP_006717517                                                           15    9605
XP_006717516                                                           15    9605
XP_005269377                                                           15    9605
XP_005269376                                                           15    9605
>NP_001895
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 270 KMAVRLAGG 278
>NP_001091680
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 270 KMAVRLAGG 278
>NP_001091679
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 270 KMAVRLAGG 278
>XP_006713048
          Length = 720

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 270 KMAVRLAGG 278
>XP_006713047
          Length = 774

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 263 KMAVRLAGG 271
>XP_006713046
          Length = 774

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 263 KMAVRLAGG 271
>XP_005264943
          Length = 781

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMAVRFAGG 9
           KMAVR AGG
Sbjct: 270 KMAVRLAGG 278
>NP_001186091
          Length = 1372

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_001186090
          Length = 1384

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_006668
          Length = 1318

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_001186089
          Length = 1419

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264888
          Length = 1268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713016
          Length = 1404

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264887
          Length = 1322

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713015
          Length = 1367

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713014
          Length = 1368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713013
          Length = 1369

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_006713012
          Length = 1373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713011
          Length = 1382

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264886
          Length = 1386

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713010
          Length = 1405

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264885
          Length = 1406

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264884
          Length = 1408

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 139 VRFAGG 144
>XP_005264883
          Length = 1421

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264882
          Length = 1421

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_006713009
          Length = 1422

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264881
          Length = 1423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>XP_005264880
          Length = 1423

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           VRFAGG
Sbjct: 154 VRFAGG 159
>NP_001518
          Length = 488

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MAVRFAGG 9
           MAV +AGG
Sbjct: 132 MAVNWAGG 139
>NP_001011705
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           +RFAGG
Sbjct: 123 IRFAGG 128
>NP_008946
          Length = 334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           +RFAGG
Sbjct: 123 IRFAGG 128
>NP_002806
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KMAVRFAG 8
           K+A+R+AG
Sbjct: 274 KLALRYAG 281
>NP_001257411
          Length = 422

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KMAVRFAG 8
           K+A+R+AG
Sbjct: 274 KLALRYAG 281
>XP_006719824
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           +RFAGG
Sbjct: 123 IRFAGG 128
>XP_006719823
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VRFAGG 9
           +RFAGG
Sbjct: 123 IRFAGG 128
>NP_653272
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  AVRFAG 8
          AVRFAG
Sbjct: 80 AVRFAG 85
>NP_001171468
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  AVRFAG 8
          AVRFAG
Sbjct: 80 AVRFAG 85
>NP_001171467
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  AVRFAG 8
          AVRFAG
Sbjct: 93 AVRFAG 98
>XP_006719320
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  AVRFAG 8
          AVRFAG
Sbjct: 80 AVRFAG 85
>NP_006438
          Length = 823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KMAVRFAG 8
           K AVRF G
Sbjct: 287 KKAVRFKG 294
>NP_001001992
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KMAVRFAG 8
           K AVRF G
Sbjct: 286 KKAVRFKG 293
>NP_821133
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KMAVRFAG 8
           KMAV F G
Sbjct: 362 KMAVTFIG 369
>NP_001027582
          Length = 823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KMAVRFAG 8
           K AVRF G
Sbjct: 287 KKAVRFKG 294
>NP_116253
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  MAVRFAGG 9
          M +RF GG
Sbjct: 52 MELRFVGG 59
>NP_060546
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 625 KEAIRKAGG 633
>NP_001276949
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 625 KEAIRKAGG 633
>NP_001276950
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 150 KEAIRKAGG 158
>NP_001274427
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AVRFAGG 9
           +V+FAGG
Sbjct: 214 SVQFAGG 220
>NP_115671
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AVRFAGG 9
           +V+FAGG
Sbjct: 214 SVQFAGG 220
>NP_996996
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AVRFAGG 9
           +V+FAGG
Sbjct: 214 SVQFAGG 220
>NP_872433
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MAVRFAGG 9
         MAV++ GG
Sbjct: 1 MAVKWTGG 8
>XP_005252542
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 317 KEAIRKAGG 325
>XP_006717518
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 317 KEAIRKAGG 325
>XP_006717517
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 508 KEAIRKAGG 516
>XP_006717516
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KMAVRFAGG 9
           K A+R AGG
Sbjct: 593 KEAIRKAGG 601
>XP_005269377
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MAVRFAGG 9
         MAV++ GG
Sbjct: 1 MAVKWTGG 8
>XP_005269376
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 MAVRFAGG 9
         MAV++ GG
Sbjct: 1 MAVKWTGG 8
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.142    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,043
Number of extensions: 380
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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