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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              19     869
NP_055590                                                              18    1135
NP_001007468                                                           18    1135
NP_001245256                                                           18    1135
NP_001245255                                                           18    1135
NP_001245254                                                           18    1135
XP_005261931                                                           18    1135
XP_006724453                                                           18    1135
XP_005261930                                                           18    1135
XP_006724452                                                           18    1135
XP_005261929                                                           18    1135
XP_005261928                                                           18    1135
XP_005261927                                                           18    1135
XP_006724451                                                           18    1135
XP_005261926                                                           18    1135
XP_005261925                                                           18    1135
NP_001018864                                                           16    7355
NP_000055                                                              16    7355
XP_005247970                                                           16    7355
XP_005247969                                                           16    7355
XP_005247968                                                           16    7355
XP_006710833                                                           16    7355
XP_006710832                                                           16    7355
NP_061174                                                              16    7355
NP_775873                                                              15    9605
XP_006719585                                                           15    9605
XP_006719584                                                           15    9605
XP_005269101                                                           15    9605
XP_006719583                                                           15    9605

>NP_004295
          Length = 1620

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KMALQRSFT 9
           KMALQ SFT
Sbjct: 434 KMALQSSFT 442



>NP_055590
          Length = 1211

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>NP_001007468
          Length = 1242

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>NP_001245256
          Length = 1148

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 267 KMALRRAFT 275



>NP_001245255
          Length = 1160

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 267 KMALRRAFT 275



>NP_001245254
          Length = 1187

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 325 KMALRRAFT 333



>XP_005261931
          Length = 871

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>XP_006724453
          Length = 1160

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 267 KMALRRAFT 275



>XP_005261930
          Length = 1189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 267 KMALRRAFT 275



>XP_006724452
          Length = 1189

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 267 KMALRRAFT 275



>XP_005261929
          Length = 1240

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>XP_005261928
          Length = 1247

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 325 KMALRRAFT 333



>XP_005261927
          Length = 1247

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 325 KMALRRAFT 333



>XP_006724451
          Length = 1271

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>XP_005261926
          Length = 1271

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>XP_005261925
          Length = 1271

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   KMALQRSFT 9
           KMAL+R+FT
Sbjct: 349 KMALRRAFT 357



>NP_001018864
          Length = 598

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MALQRSF 8
           +ALQRSF
Sbjct: 322 IALQRSF 328



>NP_000055
          Length = 1663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    MALQRSFT 9
            M LQRS+T
Sbjct: 1181 MNLQRSYT 1188



>XP_005247970
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MALQRSF 8
           +ALQRSF
Sbjct: 322 IALQRSF 328



>XP_005247969
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MALQRSF 8
           +ALQRSF
Sbjct: 138 IALQRSF 144



>XP_005247968
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MALQRSF 8
           +ALQRSF
Sbjct: 322 IALQRSF 328



>XP_006710833
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  MALQRSF 8
          MALQR F
Sbjct: 55 MALQRDF 61



>XP_006710832
          Length = 628

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  MALQRSF 8
          MALQR F
Sbjct: 55 MALQRDF 61



>NP_061174
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  MALQRSF 8
          MALQR F
Sbjct: 55 MALQRDF 61



>NP_775873
          Length = 1576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    KMALQRSFT 9
            ++ALQ+SF+
Sbjct: 1291 RVALQQSFS 1299



>XP_006719585
          Length = 1459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    KMALQRSFT 9
            ++ALQ+SF+
Sbjct: 1174 RVALQQSFS 1182



>XP_006719584
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    KMALQRSFT 9
            ++ALQ+SF+
Sbjct: 1230 RVALQQSFS 1238



>XP_005269101
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    KMALQRSFT 9
            ++ALQ+SF+
Sbjct: 1292 RVALQQSFS 1300



>XP_006719583
          Length = 1586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    KMALQRSFT 9
            ++ALQ+SF+
Sbjct: 1301 RVALQQSFS 1309



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.126    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 329,522
Number of extensions: 341
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)