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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     509
NP_001124295                                                           18    1935
NP_001370                                                              18    1935
NP_115553                                                              18    1935
XP_006722744                                                           18    1935
NP_005198                                                              17    4312
NP_036381                                                              16    5631
NP_057336                                                              16    7355
XP_006712120                                                           16    7355
XP_005264512                                                           16    7355
XP_005264511                                                           16    7355
XP_006712119                                                           16    7355
XP_005264510                                                           16    7355
XP_006712118                                                           16    7355
XP_005264509                                                           16    7355
XP_005264508                                                           16    7355
XP_006712117                                                           16    7355
XP_005264507                                                           16    7355
XP_005264506                                                           16    7355
NP_000480                                                              15    9605
NP_612114                                                              15    9605
NP_001136157                                                           15    9605
NP_001137434                                                           15    9605
XP_006724730                                                           15    9605
XP_005262214                                                           15    9605
XP_005262213                                                           15    9605
XP_006724729                                                           15    9605
XP_005262212                                                           15    9605
XP_005262211                                                           15    9605
XP_005262210                                                           15    9605
XP_005271793                                                           15    9605
NP_001035194                                                           15    9605

>NP_004351
          Length = 882

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KMALEVGD 8
           KMALEVGD
Sbjct: 655 KMALEVGD 662



>NP_001124295
          Length = 1632

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEVGDC 9
           LEVGDC
Sbjct: 773 LEVGDC 778



>NP_001370
          Length = 1616

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEVGDC 9
           LEVGDC
Sbjct: 757 LEVGDC 762



>NP_115553
          Length = 394

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2 MALEVGD 8
         MALEVGD
Sbjct: 1 MALEVGD 7



>XP_006722744
          Length = 1495

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   LEVGDC 9
           LEVGDC
Sbjct: 636 LEVGDC 641



>NP_005198
          Length = 303

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   MALEVGD 8
           +ALEVGD
Sbjct: 256 LALEVGD 262



>NP_036381
          Length = 205

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KMALEVGDC 9
           K+A E+G C
Sbjct: 150 KLAKEIGAC 158



>NP_057336
          Length = 4857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4236 RMALEIG 4242



>XP_006712120
          Length = 4812

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4191 RMALEIG 4197



>XP_005264512
          Length = 4840

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4219 RMALEIG 4225



>XP_005264511
          Length = 4847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4263 RMALEIG 4269



>XP_006712119
          Length = 4851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4230 RMALEIG 4236



>XP_005264510
          Length = 4861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4240 RMALEIG 4246



>XP_006712118
          Length = 4864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4243 RMALEIG 4249



>XP_005264509
          Length = 4870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4249 RMALEIG 4255



>XP_005264508
          Length = 4875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4254 RMALEIG 4260



>XP_006712117
          Length = 4878

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4257 RMALEIG 4263



>XP_005264507
          Length = 4880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4259 RMALEIG 4265



>XP_005264506
          Length = 4884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KMALEVG 7
            +MALE+G
Sbjct: 4263 RMALEIG 4269



>NP_000480
          Length = 2492

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 2028 RMAEEIGD 2035



>NP_612114
          Length = 2454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1990 RMAEEIGD 1997



>NP_001136157
          Length = 2087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KMALEVGD 8
           ++ LEVGD
Sbjct: 277 ELTLEVGD 284



>NP_001137434
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ALEVGD 8
           ALE+GD
Sbjct: 511 ALEIGD 516



>XP_006724730
          Length = 2399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1935 RMAEEIGD 1942



>XP_005262214
          Length = 2424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1960 RMAEEIGD 1967



>XP_005262213
          Length = 2437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1973 RMAEEIGD 1980



>XP_006724729
          Length = 2453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1989 RMAEEIGD 1996



>XP_005262212
          Length = 2453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1989 RMAEEIGD 1996



>XP_005262211
          Length = 2463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 1999 RMAEEIGD 2006



>XP_005262210
          Length = 2491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KMALEVGD 8
            +MA E+GD
Sbjct: 2027 RMAEEIGD 2034



>XP_005271793
          Length = 2061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KMALEVGD 8
           ++ LEVGD
Sbjct: 251 ELTLEVGD 258



>NP_001035194
          Length = 4493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KMALEVGD 8
            KMA E GD
Sbjct: 4319 KMAKEYGD 4326



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,565
Number of extensions: 569
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)