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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055798                                                              16    5631
NP_003698                                                              16    5631
XP_006714830                                                           16    5631
XP_006714828                                                           16    5631
NP_955781                                                              15    9605
NP_060714                                                              15    9605
NP_055325                                                              15    9605
NP_149015                                                              15    9605
XP_006723877                                                           15    9605
XP_006715989                                                           15    9605

>NP_055798
          Length = 1292

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLSTTKTKR 9
           KL+TT T+R
Sbjct: 302 KLTTTYTRR 310



>NP_003698
          Length = 456

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  STTKTKR 9
          STTKT+R
Sbjct: 8  STTKTQR 14



>XP_006714830
          Length = 1007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KLSTTKTKR 9
          KL+TT T+R
Sbjct: 17 KLTTTYTRR 25



>XP_006714828
          Length = 1126

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLSTTKTKR 9
           KL+TT T+R
Sbjct: 136 KLTTTYTRR 144



>NP_955781
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KLSTTK 6
          KLSTTK
Sbjct: 75 KLSTTK 80



>NP_060714
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KLSTTK 6
          KLSTTK
Sbjct: 75 KLSTTK 80



>NP_055325
          Length = 4935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSTTKTKR 9
            LSTTK+ R
Sbjct: 2900 LSTTKSHR 2907



>NP_149015
          Length = 5142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSTTKTKR 9
            LSTTK+ R
Sbjct: 2900 LSTTKSHR 2907



>XP_006723877
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KLSTTK 6
          KLSTTK
Sbjct: 75 KLSTTK 80



>XP_006715989
          Length = 5148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LSTTKTKR 9
            LSTTK+ R
Sbjct: 2900 LSTTKSHR 2907



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.106    0.246 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,885
Number of extensions: 394
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)