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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              19     869
NP_001073936                                                           18    1935
NP_003912                                                              17    4312
XP_005271368                                                           17    4312
NP_631905                                                              16    5631
NP_001137472                                                           16    7355
NP_001177739                                                           16    7355
NP_060358                                                              16    7355
XP_006721119                                                           16    7355
XP_006721118                                                           16    7355
NP_597704                                                              16    7355

>NP_004295
          Length = 1620

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KLRTSTIM 8
            KLRTSTIM
Sbjct: 1082 KLRTSTIM 1089



>NP_001073936
          Length = 1848

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KLRTSTIMI 9
           K RT+TIMI
Sbjct: 763 KFRTATIMI 771



>NP_003912
          Length = 233

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLRTSTIM 8
           KLR ST+M
Sbjct: 146 KLRASTVM 153



>XP_005271368
          Length = 222

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLRTSTIM 8
           KLR ST+M
Sbjct: 135 KLRASTVM 142



>NP_631905
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  KLRTSTIMI 9
          K+RT+T++I
Sbjct: 19 KIRTATLVI 27



>NP_001137472
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRTSTIMI 9
           LRTSTI +
Sbjct: 333 LRTSTIKV 340



>NP_001177739
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRTSTIMI 9
           LRTSTI +
Sbjct: 333 LRTSTIKV 340



>NP_060358
          Length = 579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTSTIMI 9
           +RT T+MI
Sbjct: 136 MRTGTVMI 143



>XP_006721119
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTSTIMI 9
           +RT T+MI
Sbjct: 136 MRTGTVMI 143



>XP_006721118
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LRTSTIMI 9
           +RT T+MI
Sbjct: 136 MRTGTVMI 143



>NP_597704
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRTSTIMI 9
           LRTSTI +
Sbjct: 172 LRTSTIKV 179



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.134    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 282,004
Number of extensions: 254
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)