Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055244                                                              18    1935
XP_005247419                                                           18    1935
NP_733751                                                              17    3301
NP_055335                                                              17    3301
NP_001099008                                                           17    3301
XP_006721482                                                           17    3301
XP_005256470                                                           17    3301
XP_006720035                                                           17    3301
XP_005268376                                                           17    3301
XP_006720034                                                           17    3301
XP_006720033                                                           17    3301
XP_005268375                                                           17    3301
XP_006720032                                                           17    3301
XP_006716142                                                           17    3301
XP_005250088                                                           17    3301
XP_006716141                                                           17    3301
XP_005250085                                                           17    3301
XP_006716140                                                           17    3301
XP_005250084                                                           17    3301
XP_005250083                                                           17    3301
XP_005250082                                                           17    3301
NP_060375                                                              17    3301
NP_000039                                                              16    7355
NP_001020117                                                           16    7355
NP_001020114                                                           16    7355
NP_001020115                                                           16    7355
NP_001153844                                                           15    9605
NP_113654                                                              15    9605
NP_066000                                                              15    9605
NP_001189367                                                           15    9605
NP_056423                                                              15    9605
NP_057129                                                              15    9605
XP_005269745                                                           15    9605
XP_006716727                                                           15    9605
XP_005251134                                                           15    9605
XP_006713781                                                           15    9605
>NP_055244
          Length = 984

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   KLKSLIGA 8
           KLKSL+GA
Sbjct: 388 KLKSLLGA 395
>XP_005247419
          Length = 1014

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   KLKSLIGA 8
           KLKSL+GA
Sbjct: 388 KLKSLLGA 395
>NP_733751
          Length = 4911

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 911 KLKSGIGAV 919
>NP_055335
          Length = 1328

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLK L+G+V
Sbjct: 228 KLKKLVGSV 236
>NP_001099008
          Length = 1332

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLK L+G+V
Sbjct: 228 KLKKLVGSV 236
>XP_006721482
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLK L+G+V
Sbjct: 228 KLKKLVGSV 236
>XP_005256470
          Length = 1120

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLK L+G+V
Sbjct: 228 KLKKLVGSV 236
>XP_006720035
          Length = 411

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>XP_005268376
          Length = 543

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>XP_006720034
          Length = 613

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>XP_006720033
          Length = 646

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>XP_005268375
          Length = 655

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>XP_006720032
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>XP_006716142
          Length = 4927

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>XP_005250088
          Length = 4928

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>XP_006716141
          Length = 4959

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>XP_005250085
          Length = 4979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>XP_006716140
          Length = 4982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>XP_005250084
          Length = 4982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>XP_005250083
          Length = 4982

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 911 KLKSGIGAV 919
>XP_005250082
          Length = 4983

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLKS IGAV
Sbjct: 912 KLKSGIGAV 920
>NP_060375
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KLKSLIGAV 9
          KLK+ IGAV
Sbjct: 70 KLKTAIGAV 78
>NP_000039
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLKSLIGA 8
           +LK LIGA
Sbjct: 95  RLKELIGA 102
>NP_001020117
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLKSLIGA 8
           +LK LIGA
Sbjct: 95  RLKELIGA 102
>NP_001020114
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLKSLIGA 8
           +LK LIGA
Sbjct: 95  RLKELIGA 102
>NP_001020115
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLKSLIGA 8
           +LK LIGA
Sbjct: 95  RLKELIGA 102
>NP_001153844
          Length = 1148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KLKSLIG 7
           K+KSL+G
Sbjct: 556 KMKSLLG 562
>NP_113654
          Length = 1246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KLKSLIG 7
           K+KSL+G
Sbjct: 654 KMKSLLG 660
>NP_066000
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KLKSLIG 7
           KLK LIG
Sbjct: 532 KLKKLIG 538
>NP_001189367
          Length = 1238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KLKSLIGAV 9
            KLK+L GA+
Sbjct: 1100 KLKTLSGAL 1108
>NP_056423
          Length = 1204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KLKSLIGAV 9
            KLK+L GA+
Sbjct: 1066 KLKTLSGAL 1074
>NP_057129
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LKSLIGA 8
          +KSLIGA
Sbjct: 65 VKSLIGA 71
>XP_005269745
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KLKSLIGAV 9
           KLK+L GA+
Sbjct: 721 KLKTLSGAL 729
>XP_006716727
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KLKSLIG 7
           K+KSL+G
Sbjct: 547 KMKSLLG 553
>XP_005251134
          Length = 1148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KLKSLIG 7
           K+KSL+G
Sbjct: 556 KMKSLLG 562
>XP_006713781
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KLKSLIG 7
           KLK LIG
Sbjct: 532 KLKKLIG 538
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.138    0.335 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 357,319
Number of extensions: 552
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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