Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1935
XP_006716142                                                           18    1935
XP_005250088                                                           18    1935
XP_006716141                                                           18    1935
XP_005250085                                                           18    1935
XP_006716140                                                           18    1935
XP_005250084                                                           18    1935
XP_005250083                                                           18    1935
XP_005250082                                                           18    1935
NP_001001662                                                           17    4312
XP_005251799                                                           17    4312
XP_005251801                                                           17    4312
XP_006717044                                                           17    4312
XP_006717043                                                           17    4312
NP_064505                                                              16    5631
NP_001164218                                                           16    5631
NP_006214                                                              16    5631
XP_006712699                                                           16    5631
XP_006712698                                                           16    5631
XP_006712697                                                           16    5631
NP_060228                                                              16    5631
NP_003449                                                              16    7355
XP_006713443                                                           16    7355
NP_877963                                                              15    9605
NP_002651                                                              15    9605
NP_071903                                                              15    9605
XP_005260495                                                           15    9605
XP_005249615                                                           15    9605
XP_005249612                                                           15    9605
NP_061166                                                              15    9605

>NP_733751
          Length = 4911

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 911 KLKSGIGAV 919



>XP_006716142
          Length = 4927

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>XP_005250088
          Length = 4928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>XP_006716141
          Length = 4959

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>XP_005250085
          Length = 4979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>XP_006716140
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>XP_005250084
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>XP_005250083
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 911 KLKSGIGAV 919



>XP_005250082
          Length = 4983

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KLKSGIRAV 9
           KLKSGI AV
Sbjct: 912 KLKSGIGAV 920



>NP_001001662
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           KLKSG+R
Sbjct: 516 KLKSGLR 522



>XP_005251799
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           KLKSG+R
Sbjct: 516 KLKSGLR 522



>XP_005251801
          Length = 675

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           KLKSG+R
Sbjct: 492 KLKSGLR 498



>XP_006717044
          Length = 699

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           KLKSG+R
Sbjct: 516 KLKSGLR 522



>XP_006717043
          Length = 711

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           KLKSG+R
Sbjct: 528 KLKSGLR 534



>NP_064505
          Length = 1555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KLKSGIRAV 9
           KLK G RAV
Sbjct: 885 KLKKGQRAV 893



>NP_001164218
          Length = 133

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  KLKSGIR 7
          KLKSG+R
Sbjct: 82 KLKSGMR 88



>NP_006214
          Length = 156

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  KLKSGIR 7
          KLKSG+R
Sbjct: 82 KLKSGMR 88



>XP_006712699
          Length = 1544

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KLKSGIRAV 9
           KLK G RAV
Sbjct: 874 KLKKGQRAV 882



>XP_006712698
          Length = 1547

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KLKSGIRAV 9
           KLK G RAV
Sbjct: 877 KLKKGQRAV 885



>XP_006712697
          Length = 1549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KLKSGIRAV 9
           KLK G RAV
Sbjct: 879 KLKKGQRAV 887



>NP_060228
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  KLKSGIR 7
          K+KSGIR
Sbjct: 84 KVKSGIR 90



>NP_003449
          Length = 3926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           K+KSG+R
Sbjct: 634 KVKSGVR 640



>XP_006713443
          Length = 3970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KLKSGIR 7
           K+KSG+R
Sbjct: 678 KVKSGVR 684



>NP_877963
          Length = 1290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LKSGIRAV 9
            LK+G RAV
Sbjct: 1183 LKTGYRAV 1190



>NP_002651
          Length = 1291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LKSGIRAV 9
            LK+G RAV
Sbjct: 1183 LKTGYRAV 1190



>NP_071903
          Length = 490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LKSGIRA 8
           LK GIRA
Sbjct: 139 LKKGIRA 145



>XP_005260495
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LKSGIRAV 9
            LK+G RAV
Sbjct: 1222 LKTGYRAV 1229



>XP_005249615
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LKSGIRA 8
           LK GIRA
Sbjct: 139 LKKGIRA 145



>XP_005249612
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LKSGIRA 8
           LK GIRA
Sbjct: 139 LKKGIRA 145



>NP_061166
          Length = 363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KLKSGIRAV 9
           KLK+GI  V
Sbjct: 218 KLKNGISVV 226



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.135    0.336 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 404,612
Number of extensions: 285
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)