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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001020461                                                           16    7355
NP_775922                                                              16    7355
NP_060250                                                              15    9605
NP_006174                                                              15    9605
NP_001129666                                                           15    9605
NP_001129665                                                           15    9605
NP_071741                                                              15    9605
NP_003794                                                              15    9605
NP_062830                                                              15    9605
XP_006716523                                                           15    9605
XP_006716522                                                           15    9605
XP_005251324                                                           15    9605
XP_005251323                                                           15    9605
XP_006714035                                                           15    9605
XP_006714034                                                           15    9605
NP_055744                                                              15    9605

>NP_001020461
          Length = 116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LEVKALEA 9
          LE+KALE+
Sbjct: 55 LELKALES 62



>NP_775922
          Length = 6996

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KLEVKALEA 9
            +L+VK LEA
Sbjct: 6691 RLDVKPLEA 6699



>NP_060250
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    EVKALEA 9
            EV+ALEA
Sbjct: 1947 EVRALEA 1953



>NP_006174
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  EVKALEA 9
          E+KALEA
Sbjct: 29 EMKALEA 35



>NP_001129666
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLEVKAL 7
           KLE+KAL
Sbjct: 328 KLEMKAL 334



>NP_001129665
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLEVKAL 7
           KLE+KAL
Sbjct: 328 KLEMKAL 334



>NP_071741
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLEVKALE 8
           K E+KALE
Sbjct: 522 KEEIKALE 529



>NP_003794
          Length = 1685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LEVKALEA 9
           L+VK LEA
Sbjct: 514 LDVKCLEA 521



>NP_062830
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LEVKALEA 9
           L+VK LEA
Sbjct: 514 LDVKCLEA 521



>XP_006716523
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVKALEA 9
           EV+ALEA
Sbjct: 862 EVRALEA 868



>XP_006716522
          Length = 2192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    EVKALEA 9
            EV+ALEA
Sbjct: 1142 EVRALEA 1148



>XP_005251324
          Length = 2996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    EVKALEA 9
            EV+ALEA
Sbjct: 1947 EVRALEA 1953



>XP_005251323
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    EVKALEA 9
            EV+ALEA
Sbjct: 1947 EVRALEA 1953



>XP_006714035
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLEVKALE 8
           K E+KALE
Sbjct: 522 KEEIKALE 529



>XP_006714034
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KLEVKALE 8
           K E+KALE
Sbjct: 522 KEEIKALE 529



>NP_055744
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KLEVKAL 7
           KLE+KAL
Sbjct: 328 KLEMKAL 334



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.121    0.270 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 331,447
Number of extensions: 463
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)