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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              20     509
NP_113609                                                              18    1482
XP_006722192                                                           18    1482
NP_008949                                                              17    3301
XP_006717007                                                           17    3301
XP_006717004                                                           17    3301
XP_006717003                                                           17    3301
XP_005251736                                                           17    3301
NP_056025                                                              16    5631
XP_006722382                                                           16    5631
XP_006722381                                                           16    5631
XP_005258156                                                           16    5631
XP_005258155                                                           16    5631
NP_002418                                                              16    7355
NP_001265446                                                           16    7355
NP_002384                                                              16    7355
NP_055693                                                              16    7355
NP_653169                                                              16    7355
NP_001094284                                                           16    7355
XP_006726605                                                           16    7355
XP_006726509                                                           16    7355
XP_006719242                                                           16    7355
XP_006716286                                                           16    7355
XP_006711392                                                           16    7355
XP_006710022                                                           16    7355
NP_073739                                                              15    9605
NP_056039                                                              15    9605
NP_001106207                                                           15    9605
XP_006713141                                                           15    9605
XP_006713140                                                           15    9605
XP_005265056                                                           15    9605

>NP_004295
          Length = 1620

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KKVSEVGRE 9
           KK SEVGRE
Sbjct: 193 KKASEVGRE 201



>NP_113609
          Length = 672

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   KKVSEVGR 8
           KK+SEVGR
Sbjct: 636 KKLSEVGR 643



>XP_006722192
          Length = 509

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   KKVSEVGR 8
           KK+SEVGR
Sbjct: 473 KKLSEVGR 480



>NP_008949
          Length = 2325

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKVSEVGRE 9
           KK+SE G++
Sbjct: 484 KKISEAGKD 492



>XP_006717007
          Length = 1499

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKVSEVGRE 9
           KK+SE G++
Sbjct: 484 KKISEAGKD 492



>XP_006717004
          Length = 2240

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKVSEVGRE 9
           KK+SE G++
Sbjct: 484 KKISEAGKD 492



>XP_006717003
          Length = 2278

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKVSEVGRE 9
           KK+SE G++
Sbjct: 484 KKISEAGKD 492



>XP_005251736
          Length = 2325

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKVSEVGRE 9
           KK+SE G++
Sbjct: 484 KKISEAGKD 492



>NP_056025
          Length = 1586

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KKVSEVGRE 9
           KK++++GRE
Sbjct: 161 KKMAKLGRE 169



>XP_006722382
          Length = 1644

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KKVSEVGRE 9
           KK++++GRE
Sbjct: 182 KKMAKLGRE 190



>XP_006722381
          Length = 1672

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KKVSEVGRE 9
           KK++++GRE
Sbjct: 182 KKMAKLGRE 190



>XP_005258156
          Length = 1905

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KKVSEVGRE 9
           KK++++GRE
Sbjct: 521 KKMAKLGRE 529



>XP_005258155
          Length = 1970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   KKVSEVGRE 9
           KK++++GRE
Sbjct: 521 KKMAKLGRE 529



>NP_002418
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KKVSEVG 7
           KK+SE+G
Sbjct: 368 KKISELG 374



>NP_001265446
          Length = 392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           KKV EVG+
Sbjct: 175 KKVIEVGK 182



>NP_002384
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           KKV EVG+
Sbjct: 273 KKVIEVGK 280



>NP_055693
          Length = 648

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKVSEVGRE 9
           KK+ E GRE
Sbjct: 566 KKMKENGRE 574



>NP_653169
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           +KVS +GR
Sbjct: 198 RKVSSIGR 205



>NP_001094284
          Length = 284

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           +KVS +GR
Sbjct: 205 RKVSSIGR 212



>XP_006726605
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KVSEVGRE 9
           KVSE+G E
Sbjct: 142 KVSEMGSE 149



>XP_006726509
          Length = 142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  KKVSEVGRE 9
          + +SE+G+E
Sbjct: 76 RMISEIGKE 84



>XP_006719242
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KVSEVGRE 9
           KVSE+G E
Sbjct: 142 KVSEMGSE 149



>XP_006716286
          Length = 142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  KKVSEVGRE 9
          + +SE+G+E
Sbjct: 76 RMISEIGKE 84



>XP_006711392
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           KKV EVG+
Sbjct: 273 KKVIEVGK 280



>XP_006710022
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KVSEVGRE 9
           KVSE+G E
Sbjct: 142 KVSEMGSE 149



>NP_073739
          Length = 1430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    SEVGRE 9
            SE+GRE
Sbjct: 1350 SEIGRE 1355



>NP_056039
          Length = 1233

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           KK+ E+GR
Sbjct: 936 KKLKELGR 943



>NP_001106207
          Length = 1512

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KKVSEVGR 8
            KK+ E+GR
Sbjct: 1373 KKLKELGR 1380



>XP_006713141
          Length = 1274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KKVSEVGR 8
           KK+ E+GR
Sbjct: 977 KKLKELGR 984



>XP_006713140
          Length = 1629

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KKVSEVGR 8
            KK+ E+GR
Sbjct: 1332 KKLKELGR 1339



>XP_005265056
          Length = 1670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KKVSEVGR 8
            KK+ E+GR
Sbjct: 1373 KKLKELGR 1380



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.121    0.289 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 411,577
Number of extensions: 502
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)