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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006711326                                                           16    5631
XP_005273145                                                           16    5631
XP_006711325                                                           16    5631
NP_699189                                                              16    7355
NP_001879                                                              15    9605
NP_006744                                                              15    9605
NP_001014444                                                           15    9605

>XP_006711326
          Length = 694

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKTAGVSAK 9
           KKT  +SAK
Sbjct: 339 KKTEAISAK 347



>XP_005273145
          Length = 845

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKTAGVSAK 9
           KKT  +SAK
Sbjct: 339 KKTEAISAK 347



>XP_006711325
          Length = 864

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKTAGVSAK 9
           KKT  +SAK
Sbjct: 339 KKTEAISAK 347



>NP_699189
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KKTAGV 6
          KKTAGV
Sbjct: 92 KKTAGV 97



>NP_001879
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKTAGVSA 8
           K+TA VSA
Sbjct: 118 KRTAAVSA 125



>NP_006744
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKTAGVSAK 9
           K+TAGV  K
Sbjct: 309 KRTAGVVEK 317



>NP_001014444
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  KKTAGVSA 8
          K+TA VSA
Sbjct: 76 KRTAAVSA 83



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.105    0.248 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 351,972
Number of extensions: 316
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)