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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004027                                                              17    2528
NP_056262                                                              17    2528
XP_005263454                                                           17    2528
XP_005263453                                                           17    2528
XP_006711990                                                           17    2528
XP_005264162                                                           17    2528
XP_005264161                                                           17    2528
XP_005264159                                                           17    2528
XP_006711989                                                           17    2528
XP_006711988                                                           17    2528
NP_005276                                                              17    4312
NP_000673                                                              16    7355
NP_000672                                                              16    7355
NP_001254711                                                           16    7355
NP_001254712                                                           16    7355
NP_060219                                                              16    7355
NP_000674                                                              16    7355
XP_006718323                                                           16    7355
XP_005253071                                                           16    7355
XP_005253069                                                           16    7355
XP_005253068                                                           16    7355
XP_006718322                                                           16    7355
XP_005253066                                                           16    7355
NP_001139669                                                           16    7355
NP_000294                                                              15    9605
NP_059138                                                              15    9605
NP_002667                                                              15    9605
NP_001137505                                                           15    9605
XP_005261699                                                           15    9605
XP_005261698                                                           15    9605
XP_005261694                                                           15    9605
XP_005255431                                                           15    9605
XP_005255430                                                           15    9605
XP_005255429                                                           15    9605
XP_005266331                                                           15    9605
NP_660329                                                              15    9605

>NP_004027
          Length = 1144

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 604 KKRNTFLLS 612



>NP_056262
          Length = 1602

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    KKRATFLL 8
            KKRA FLL
Sbjct: 1285 KKRAAFLL 1292



>XP_005263454
          Length = 1324

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    KKRATFLL 8
            KKRA FLL
Sbjct: 1007 KKRAAFLL 1014



>XP_005263453
          Length = 1613

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    KKRATFLL 8
            KKRA FLL
Sbjct: 1296 KKRAAFLL 1303



>XP_006711990
          Length = 1131

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 626 KKRNTFLLS 634



>XP_005264162
          Length = 1144

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 604 KKRNTFLLS 612



>XP_005264161
          Length = 1145

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 604 KKRNTFLLS 612



>XP_005264159
          Length = 1166

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 626 KKRNTFLLS 634



>XP_006711989
          Length = 1167

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 626 KKRNTFLLS 634



>XP_006711988
          Length = 1167

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           KKR TFLL+
Sbjct: 626 KKRNTFLLS 634



>NP_005276
          Length = 328

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KKRATFLL 8
           KKR TFL+
Sbjct: 246 KKRVTFLV 253



>NP_000673
          Length = 450

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           +KR TF+LA
Sbjct: 366 EKRFTFVLA 374



>NP_000672
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           +KR TF+LA
Sbjct: 384 EKRFTFVLA 392



>NP_001254711
          Length = 1301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>NP_001254712
          Length = 1179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>NP_060219
          Length = 1208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>NP_000674
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKRATFLLA 9
           +KR TF+LA
Sbjct: 377 EKRFTFVLA 385



>XP_006718323
          Length = 1119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>XP_005253071
          Length = 1208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>XP_005253069
          Length = 1238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>XP_005253068
          Length = 1269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>XP_006718322
          Length = 1298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>XP_005253066
          Length = 1298

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          R+TFLLA
Sbjct: 54 RSTFLLA 60



>NP_001139669
          Length = 7081

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KKRATFLL 8
            KKR TF L
Sbjct: 1350 KKRVTFAL 1357



>NP_000294
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KKRATFL 7
           KKR TF+
Sbjct: 114 KKRGTFI 120



>NP_059138
          Length = 1032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RATFLLA 9
           RA+FLLA
Sbjct: 948 RASFLLA 954



>NP_002667
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KKRATFL 7
           KKR TF+
Sbjct: 123 KKRGTFI 129



>NP_001137505
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RATFLLA 9
           +ATFLLA
Sbjct: 129 QATFLLA 135



>XP_005261699
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  KKRATFL 7
          KKR TF+
Sbjct: 8  KKRGTFI 14



>XP_005261698
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  KKRATFL 7
          KKR TF+
Sbjct: 8  KKRGTFI 14



>XP_005261694
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KKRATFL 7
           KKR TF+
Sbjct: 149 KKRGTFI 155



>XP_005255431
          Length = 163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  KKRATFL 7
          KKR TF+
Sbjct: 31 KKRGTFI 37



>XP_005255430
          Length = 187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  KKRATFL 7
          KKR TF+
Sbjct: 31 KKRGTFI 37



>XP_005255429
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KKRATFL 7
           KKR TF+
Sbjct: 114 KKRGTFI 120



>XP_005266331
          Length = 241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  RATFLLA 9
          +ATFLLA
Sbjct: 88 QATFLLA 94



>NP_660329
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RATFLLA 9
           +ATFLLA
Sbjct: 138 QATFLLA 144



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.137    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 385,244
Number of extensions: 509
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)