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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     869
NP_000242                                                              19     869
XP_005264389                                                           19     869
NP_149091                                                              16    7355
NP_060988                                                              16    7355
NP_877428                                                              16    7355
NP_056227                                                              16    7355
NP_236914                                                              16    7355
NP_001074                                                              16    7355
NP_246273                                                              16    7355
XP_005247335                                                           16    7355
XP_005247333                                                           16    7355
XP_005247330                                                           16    7355
NP_000375                                                              15    9605

>NP_001245210
          Length = 868

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KKIQSTLIS 9
           KK+QSTLIS
Sbjct: 424 KKMQSTLIS 432



>NP_000242
          Length = 934

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KKIQSTLIS 9
           KK+QSTLIS
Sbjct: 490 KKMQSTLIS 498



>XP_005264389
          Length = 921

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KKIQSTLIS 9
           KK+QSTLIS
Sbjct: 490 KKMQSTLIS 498



>NP_149091
          Length = 859

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   IQSTLIS 9
           +QSTLIS
Sbjct: 836 VQSTLIS 842



>NP_060988
          Length = 874

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KKIQSTL 7
           KKIQ+TL
Sbjct: 480 KKIQTTL 486



>NP_877428
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KKIQSTL 7
           KKIQ+TL
Sbjct: 481 KKIQTTL 487



>NP_056227
          Length = 567

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKIQSTLI 8
           +K+QSTL+
Sbjct: 459 RKLQSTLL 466



>NP_236914
          Length = 833

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKIQSTLIS 9
           KKI +T+IS
Sbjct: 310 KKIAATIIS 318



>NP_001074
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKIQSTLIS 9
           KKI +T+IS
Sbjct: 352 KKIAATIIS 360



>NP_246273
          Length = 823

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKIQSTLIS 9
           KKI +T+IS
Sbjct: 300 KKIAATIIS 308



>XP_005247335
          Length = 400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKIQSTLI 8
           +K+QSTL+
Sbjct: 292 RKLQSTLL 299



>XP_005247333
          Length = 431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKIQSTLI 8
           +K+QSTL+
Sbjct: 323 RKLQSTLL 330



>XP_005247330
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KKIQSTLI 8
           +K+QSTL+
Sbjct: 389 RKLQSTLL 396



>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KKIQSTLI 8
            KK+QST +
Sbjct: 4540 KKLQSTTV 4547



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.120    0.262 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 304,027
Number of extensions: 281
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)