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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005274255                                                           17    3301
NP_001273004                                                           16    7355
NP_004311                                                              16    7355
NP_775293                                                              16    7355
NP_733841                                                              15    9605
NP_004671                                                              15    9605
NP_001003895                                                           15    9605
NP_004816                                                              15    9605
NP_001003894                                                           15    9605
NP_001001722                                                           15    9605

>XP_005274255
          Length = 287

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KKIMIMI 7
           KKIMIMI
Sbjct: 263 KKIMIMI 269



>NP_001273004
          Length = 869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +KIM +IKE
Sbjct: 418 EKIMAVIKE 426



>NP_004311
          Length = 994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +KIM +IKE
Sbjct: 543 EKIMAVIKE 551



>NP_775293
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +KIM +IKE
Sbjct: 543 EKIMAVIKE 551



>NP_733841
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +++MI IKE
Sbjct: 473 QEVMIQIKE 481



>NP_004671
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +++MI IKE
Sbjct: 473 QEVMIQIKE 481



>NP_001003895
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +++MI IKE
Sbjct: 473 QEVMIQIKE 481



>NP_004816
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +++MI IKE
Sbjct: 474 QEVMIQIKE 482



>NP_001003894
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +++MI IKE
Sbjct: 473 QEVMIQIKE 481



>NP_001001722
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKIMIMIKE 9
           +++MI IKE
Sbjct: 474 QEVMIQIKE 482



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.143    0.335 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 241,222
Number of extensions: 394
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)