Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1135
XP_006716142                                                           18    1135
XP_005250088                                                           18    1135
XP_006716141                                                           18    1135
XP_005250085                                                           18    1135
XP_006716140                                                           18    1135
XP_005250084                                                           18    1135
XP_005250083                                                           18    1135
XP_005250082                                                           18    1135
NP_659547                                                              16    5631
NP_597701                                                              16    5631
NP_001092809                                                           16    5631
NP_444269                                                              16    5631
XP_005276260                                                           16    5631
XP_005260357                                                           16    5631
XP_005260356                                                           16    5631
XP_005255220                                                           16    5631
XP_006721086                                                           16    5631
XP_005264734                                                           16    5631
XP_005275976                                                           16    5631
NP_997294                                                              16    7355
NP_001276902                                                           16    7355
NP_003473                                                              15    9605
NP_597719                                                              15    9605
NP_079416                                                              15    9605
XP_006723657                                                           15    9605
XP_006722222                                                           15    9605
XP_006722221                                                           15    9605
XP_006722220                                                           15    9605
XP_006722219                                                           15    9605
XP_006722218                                                           15    9605
XP_006722217                                                           15    9605
XP_006722216                                                           15    9605
XP_006722215                                                           15    9605
XP_006722214                                                           15    9605
XP_006719679                                                           15    9605
XP_005269219                                                           15    9605
XP_006719678                                                           15    9605
XP_006719677                                                           15    9605
XP_005253682                                                           15    9605
XP_006719674                                                           15    9605
XP_005253681                                                           15    9605
XP_006713569                                                           15    9605
NP_055053                                                              15    9605
>NP_733751
          Length = 4911

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3209 KKALSAKQ 3216
>XP_006716142
          Length = 4927

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>XP_005250088
          Length = 4928

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>XP_006716141
          Length = 4959

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>XP_005250085
          Length = 4979

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>XP_006716140
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>XP_005250084
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>XP_005250083
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3225 KKALSAKQ 3232
>XP_005250082
          Length = 4983

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KKALSAKQ 8
            KKALSAKQ
Sbjct: 3226 KKALSAKQ 3233
>NP_659547
          Length = 266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKALSAKQH 9
           KK+L ++QH
Sbjct: 258 KKSLRSRQH 266
>NP_597701
          Length = 670

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKALSAKQH 9
           KKA S +QH
Sbjct: 342 KKAASLEQH 350
>NP_001092809
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  KKALSAKQ 8
          KK+L+AKQ
Sbjct: 83 KKSLTAKQ 90
>NP_444269
          Length = 200

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  KKALSAKQ 8
          KK+L+AKQ
Sbjct: 84 KKSLTAKQ 91
>XP_005276260
          Length = 160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ALSAKQH 9
          ALSA+QH
Sbjct: 34 ALSAQQH 40
>XP_005260357
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  KKALSAKQ 8
          KK+L+AKQ
Sbjct: 83 KKSLTAKQ 90
>XP_005260356
          Length = 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  KKALSAKQ 8
          KK+L+AKQ
Sbjct: 84 KKSLTAKQ 91
>XP_005255220
          Length = 266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKALSAKQH 9
           KK+L ++QH
Sbjct: 258 KKSLRSRQH 266
>XP_006721086
          Length = 266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KKALSAKQH 9
           KK+L ++QH
Sbjct: 258 KKSLRSRQH 266
>XP_005264734
          Length = 160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ALSAKQH 9
          ALSA+QH
Sbjct: 34 ALSAQQH 40
>XP_005275976
          Length = 160

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ALSAKQH 9
          ALSA+QH
Sbjct: 34 ALSAQQH 40
>NP_997294
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KKALSAKQ 8
           +KALSA+Q
Sbjct: 446 RKALSAQQ 453
>NP_001276902
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KKALSAKQ 8
           +KALSA+Q
Sbjct: 283 RKALSAQQ 290
>NP_003473
          Length = 5537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KKALSAKQ 8
            +KAL AKQ
Sbjct: 3539 RKALCAKQ 3546
>NP_597719
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALSAKQH 9
           ALS+K+H
Sbjct: 731 ALSSKEH 737
>NP_079416
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKALSAKQH 9
           KK+LSA  H
Sbjct: 207 KKSLSAPLH 215
>XP_006723657
          Length = 259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  KKALSAKQH 9
          +KA  AK+H
Sbjct: 10 RKARGAKEH 18
>XP_006722222
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  KALSAKQH 9
          KAL+ K+H
Sbjct: 92 KALTKKEH 99
>XP_006722221
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 291 KALTKKEH 298
>XP_006722220
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 309 KALTKKEH 316
>XP_006722219
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 428 KALTKKEH 435
>XP_006722218
          Length = 877

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 428 KALTKKEH 435
>XP_006722217
          Length = 887

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 428 KALTKKEH 435
>XP_006722216
          Length = 901

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 428 KALTKKEH 435
>XP_006722215
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 428 KALTKKEH 435
>XP_006722214
          Length = 928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 428 KALTKKEH 435
>XP_006719679
          Length = 5536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KKALSAKQ 8
            +KAL AKQ
Sbjct: 3538 RKALCAKQ 3545
>XP_005269219
          Length = 5537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KKALSAKQ 8
            +KAL AKQ
Sbjct: 3539 RKALCAKQ 3546
>XP_006719678
          Length = 5539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KKALSAKQ 8
            +KAL AKQ
Sbjct: 3541 RKALCAKQ 3548
>XP_006719677
          Length = 5540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KKALSAKQ 8
            +KAL AKQ
Sbjct: 3542 RKALCAKQ 3549
>XP_005253682
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKALSAKQH 9
           KK+LSA  H
Sbjct: 207 KKSLSAPLH 215
>XP_006719674
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKALSAKQH 9
           KK+LSA  H
Sbjct: 207 KKSLSAPLH 215
>XP_005253681
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KKALSAKQH 9
           KK+LSA  H
Sbjct: 207 KKSLSAPLH 215
>XP_006713569
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ALSAKQH 9
           ALS+K+H
Sbjct: 302 ALSSKEH 308
>NP_055053
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KALSAKQH 9
           KAL+ K+H
Sbjct: 291 KALTKKEH 298
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.107    0.265 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 324,638
Number of extensions: 495
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package