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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     665
XP_005266703                                                           19     665
NP_065829                                                              16    5631
NP_803879                                                              16    7355
NP_803881                                                              16    7355
NP_001258992                                                           16    7355
NP_110428                                                              16    7355
NP_001229291                                                           16    7355
NP_001258991                                                           16    7355
NP_001073341                                                           16    7355
NP_001092270                                                           16    7355
NP_001258990                                                           16    7355
NP_003579                                                              16    7355
XP_006724848                                                           16    7355
XP_006724847                                                           16    7355
XP_005262538                                                           16    7355
XP_006724671                                                           16    7355
XP_006724670                                                           16    7355
NP_115495                                                              15    9605
NP_002284                                                              15    9605
NP_068766                                                              15    9605
NP_597732                                                              15    9605
XP_006722741                                                           15    9605
XP_006722740                                                           15    9605
XP_006722739                                                           15    9605
XP_006722738                                                           15    9605
XP_006722737                                                           15    9605
XP_005259567                                                           15    9605
XP_006721630                                                           15    9605
XP_006721629                                                           15    9605
XP_006721628                                                           15    9605

>NP_443179
          Length = 2170

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KIYAAEAQ 8
            KIYAAEAQ
Sbjct: 1993 KIYAAEAQ 2000



>XP_005266703
          Length = 1501

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KIYAAEAQ 8
            KIYAAEAQ
Sbjct: 1324 KIYAAEAQ 1331



>NP_065829
          Length = 1907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KIYAAEAQ 8
            KI+AAEA+
Sbjct: 1685 KIFAAEAR 1692



>NP_803879
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_803881
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_001258992
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_110428
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_001229291
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_001258991
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_001073341
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIYAAEAQ 8
           ++YAAE Q
Sbjct: 377 RLYAAEGQ 384



>NP_001092270
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_001258990
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_003579
          Length = 913

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIYAAEAQ 8
           ++YAAE Q
Sbjct: 395 RLYAAEGQ 402



>XP_006724848
          Length = 900

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIYAAEAQ 8
           ++YAAE Q
Sbjct: 382 RLYAAEGQ 389



>XP_006724847
          Length = 902

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIYAAEAQ 8
           ++YAAE Q
Sbjct: 382 RLYAAEGQ 389



>XP_005262538
          Length = 915

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KIYAAEAQ 8
           ++YAAE Q
Sbjct: 395 RLYAAEGQ 402



>XP_006724671
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>XP_006724670
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   YAAEAQ 8
           YAAEAQ
Sbjct: 152 YAAEAQ 157



>NP_115495
          Length = 6306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KIYAAEAQL 9
            +IYAAE ++
Sbjct: 5841 RIYAAEPRI 5849



>NP_002284
          Length = 1609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KIYAAEAQL 9
            +IYA+ AQL
Sbjct: 1266 EIYASVAQL 1274



>NP_068766
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KIYAAE 6
           K+YAAE
Sbjct: 205 KVYAAE 210



>NP_597732
          Length = 1146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YAAEAQL 9
            +AAEAQL
Sbjct: 1014 HAAEAQL 1020



>XP_006722741
          Length = 1117

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   YAAEAQL 9
           +AAEAQL
Sbjct: 985 HAAEAQL 991



>XP_006722740
          Length = 1153

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YAAEAQL 9
            +AAEAQL
Sbjct: 1021 HAAEAQL 1027



>XP_006722739
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YAAEAQL 9
            +AAEAQL
Sbjct: 1049 HAAEAQL 1055



>XP_006722738
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YAAEAQL 9
            +AAEAQL
Sbjct: 1049 HAAEAQL 1055



>XP_006722737
          Length = 1182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YAAEAQL 9
            +AAEAQL
Sbjct: 1050 HAAEAQL 1056



>XP_005259567
          Length = 1217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    YAAEAQL 9
            +AAEAQL
Sbjct: 1085 HAAEAQL 1091



>XP_006721630
          Length = 191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  KIYAAE 6
          K+YAAE
Sbjct: 56 KVYAAE 61



>XP_006721629
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KIYAAE 6
           K+YAAE
Sbjct: 205 KVYAAE 210



>XP_006721628
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KIYAAE 6
           K+YAAE
Sbjct: 205 KVYAAE 210



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.123    0.301 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 308,721
Number of extensions: 270
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)