Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     869
XP_005262424                                                           19     869
NP_001258698                                                           17    3301
NP_003655                                                              17    3301
XP_005248675                                                           17    3301
NP_644671                                                              16    7355
NP_009330                                                              16    7355
NP_060729                                                              16    7355
NP_036250                                                              16    7355
XP_003960172                                                           16    7355
XP_005253074                                                           16    7355
XP_006718326                                                           16    7355
XP_005253073                                                           16    7355
XP_006712781                                                           16    7355
XP_003119008                                                           16    7355
XP_003119853                                                           16    7355
NP_000283                                                              15    9605
NP_061135                                                              15    9605
NP_602296                                                              15    9605
XP_006724561                                                           15    9605
XP_005274607                                                           15    9605
XP_005274605                                                           15    9605
XP_006724560                                                           15    9605
XP_006724559                                                           15    9605
XP_005253784                                                           15    9605
XP_005253782                                                           15    9605
XP_006719060                                                           15    9605
XP_006719059                                                           15    9605
XP_006719058                                                           15    9605
XP_005253778                                                           15    9605
XP_005253777                                                           15    9605
XP_006715571                                                           15    9605
NP_002535                                                              15    9605
>NP_722576
          Length = 3080

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KIVNLANIT 9
            KIV+LANIT
Sbjct: 2454 KIVDLANIT 2462
>XP_005262424
          Length = 2875

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KIVNLANIT 9
            KIV+LANIT
Sbjct: 2249 KIVDLANIT 2257
>NP_001258698
          Length = 1045

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KIVNLANI 8
           K+VN+AN+
Sbjct: 976 KVVNVANV 983
>NP_003655
          Length = 1094

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KIVNLANI 8
            K+VN+AN+
Sbjct: 1025 KVVNVANV 1032
>XP_005248675
          Length = 1053

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    KIVNLANI 8
            K+VN+AN+
Sbjct: 1025 KVVNVANV 1032
>NP_644671
          Length = 712

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KIVNLANIT 9
           KI+ L N+T
Sbjct: 216 KIIELLNVT 224
>NP_009330
          Length = 750

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KIVNLANIT 9
           KI+ L N+T
Sbjct: 216 KIIELLNVT 224
>NP_060729
          Length = 1141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 265 LVNLANV 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 658 LVNLANL 664
>NP_036250
          Length = 431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KIVNLANI 8
           K+VN ANI
Sbjct: 246 KLVNSANI 253
>XP_003960172
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLANIT 9
           NLANIT
Sbjct: 389 NLANIT 394
>XP_005253074
          Length = 956

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 265 LVNLANV 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 658 LVNLANL 664
>XP_006718326
          Length = 968

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IVNLANI 8
          +VNLAN+
Sbjct: 35 LVNLANV 41

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 428 LVNLANL 434
>XP_005253073
          Length = 1198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 265 LVNLANV 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IVNLANI 8
           +VNLAN+
Sbjct: 658 LVNLANL 664
>XP_006712781
          Length = 750

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KIVNLANIT 9
           KI+ L N+T
Sbjct: 216 KIIELLNVT 224
>XP_003119008
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLANIT 9
           NLANIT
Sbjct: 389 NLANIT 394
>XP_003119853
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   NLANIT 9
           NLANIT
Sbjct: 389 NLANIT 394
>NP_000283
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL N+T
Sbjct: 273 VNLVNVT 279
>NP_061135
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL NIT
Sbjct: 385 VNLKNIT 391
>NP_602296
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_006724561
          Length = 1061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  VNLANIT 9
          VNL N+T
Sbjct: 91 VNLVNVT 97
>XP_005274607
          Length = 1207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL N+T
Sbjct: 273 VNLVNVT 279
>XP_005274605
          Length = 1217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL N+T
Sbjct: 273 VNLVNVT 279
>XP_006724560
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL N+T
Sbjct: 273 VNLVNVT 279
>XP_006724559
          Length = 1243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL N+T
Sbjct: 273 VNLVNVT 279
>XP_005253784
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_005253782
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_006719060
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_006719059
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_006719058
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_005253778
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_005253777
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  KIVNLAN 7
          +++NLAN
Sbjct: 70 RVINLAN 76
>XP_006715571
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VNLANIT 9
           VNL NIT
Sbjct: 365 VNLKNIT 371
>NP_002535
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IVNLANIT 9
           ++NL N+T
Sbjct: 187 LINLTNLT 194
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.133    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 296,757
Number of extensions: 542
Number of successful extensions: 37
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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