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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              18    1135
XP_005273257                                                           18    1135
NP_001268451                                                           17    3301
NP_001268449                                                           17    3301
NP_877954                                                              17    3301
NP_001268450                                                           17    3301
NP_115874                                                              17    3301
NP_079350                                                              17    4312
XP_006714380                                                           17    4312
XP_006714379                                                           17    4312
XP_006714378                                                           17    4312
XP_006714377                                                           17    4312
NP_001189394                                                           16    5631
NP_001189393                                                           16    5631
NP_006615                                                              16    5631
NP_001189395                                                           16    5631
NP_001360                                                              16    5631
NP_001189396                                                           16    5631
NP_997644                                                              16    5631
NP_001189397                                                           16    5631
XP_005252420                                                           16    5631
XP_006717439                                                           16    5631
XP_005252419                                                           16    5631
XP_005252418                                                           16    5631
XP_005252417                                                           16    5631
XP_005252416                                                           16    5631
XP_005248319                                                           16    5631
NP_004989                                                              16    7355
NP_005906                                                              16    7355
NP_001269723                                                           15    9605
NP_055705                                                              15    9605
NP_001269722                                                           15    9605
NP_001035924                                                           15    9605
NP_878913                                                              15    9605
XP_006713549                                                           15    9605

>NP_064450
          Length = 1722

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KITTRQLSS 9
           K TTRQLSS
Sbjct: 420 KTTTRQLSS 428



>XP_005273257
          Length = 1726

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KITTRQLSS 9
           K TTRQLSS
Sbjct: 420 KTTTRQLSS 428



>NP_001268451
          Length = 793

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ITTRQLSS 9
           ITTRQL S
Sbjct: 661 ITTRQLES 668



>NP_001268449
          Length = 840

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ITTRQLSS 9
           ITTRQL S
Sbjct: 708 ITTRQLES 715



>NP_877954
          Length = 824

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ITTRQLSS 9
           ITTRQL S
Sbjct: 692 ITTRQLES 699



>NP_001268450
          Length = 880

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ITTRQLSS 9
           ITTRQL S
Sbjct: 748 ITTRQLES 755



>NP_115874
          Length = 840

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ITTRQLSS 9
           ITTRQL S
Sbjct: 708 ITTRQLES 715



>NP_079350
          Length = 4012

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    KITTRQLSS 9
            KITT QLS+
Sbjct: 2217 KITTLQLSA 2225



>XP_006714380
          Length = 3897

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    KITTRQLSS 9
            KITT QLS+
Sbjct: 2102 KITTLQLSA 2110



>XP_006714379
          Length = 3936

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    KITTRQLSS 9
            KITT QLS+
Sbjct: 2217 KITTLQLSA 2225



>XP_006714378
          Length = 3965

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    KITTRQLSS 9
            KITT QLS+
Sbjct: 2170 KITTLQLSA 2178



>XP_006714377
          Length = 4010

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    KITTRQLSS 9
            KITT QLS+
Sbjct: 2215 KITTLQLSA 2223



>NP_001189394
          Length = 517

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>NP_001189393
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>NP_006615
          Length = 602

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>NP_001189395
          Length = 547

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>NP_001360
          Length = 4624

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ITTRQLSS 9
            +TTR LSS
Sbjct: 1873 VTTRDLSS 1880



>NP_001189396
          Length = 514

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>NP_997644
          Length = 567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>NP_001189397
          Length = 568

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_005252420
          Length = 547

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_006717439
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_005252419
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_005252418
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_005252417
          Length = 601

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_005252416
          Length = 602

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  KITTRQLS 8
          K TTRQLS
Sbjct: 50 KETTRQLS 57



>XP_005248319
          Length = 4609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    ITTRQLSS 9
            +TTR LSS
Sbjct: 1858 VTTRDLSS 1865



>NP_004989
          Length = 1108

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KITTRQLSS 9
           K+T+RQ+ S
Sbjct: 322 KLTSRQMDS 330



>NP_005906
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KITTRQLSS 9
           +IT RQL S
Sbjct: 615 RITVRQLES 623



>NP_001269723
          Length = 895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KITTRQLS 8
           ++TT+QLS
Sbjct: 212 RMTTKQLS 219



>NP_055705
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KITTRQLS 8
           ++TT+QLS
Sbjct: 212 RMTTKQLS 219



>NP_001269722
          Length = 711

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  KITTRQLS 8
          ++TT+QLS
Sbjct: 28 RMTTKQLS 35



>NP_001035924
          Length = 1133

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KITTRQLS 8
           ++TT+QLS
Sbjct: 452 RMTTKQLS 459



>NP_878913
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KITTRQLS 8
           ++TT+QLS
Sbjct: 452 RMTTKQLS 459



>XP_006713549
          Length = 893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KITTRQLS 8
           ++TT+QLS
Sbjct: 212 RMTTKQLS 219



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.112    0.252 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 291,963
Number of extensions: 266
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)