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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001273004                                                           15    9605
NP_004311                                                              15    9605
NP_775293                                                              15    9605
NP_733841                                                              15    9605
NP_004671                                                              15    9605
NP_001003895                                                           15    9605
NP_004816                                                              15    9605
NP_703147                                                              15    9605
NP_001152860                                                           15    9605
NP_001152859                                                           15    9605
NP_001003894                                                           15    9605
NP_001001722                                                           15    9605

>NP_001273004
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KIMIMIKE 8
           KIM +IKE
Sbjct: 419 KIMAVIKE 426



>NP_004311
          Length = 994

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KIMIMIKE 8
           KIM +IKE
Sbjct: 544 KIMAVIKE 551



>NP_775293
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KIMIMIKE 8
           KIM +IKE
Sbjct: 544 KIMAVIKE 551



>NP_733841
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           ++MI IKE+
Sbjct: 474 EVMIQIKEL 482



>NP_004671
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           ++MI IKE+
Sbjct: 474 EVMIQIKEL 482



>NP_001003895
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           ++MI IKE+
Sbjct: 474 EVMIQIKEL 482



>NP_004816
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           ++MI IKE+
Sbjct: 475 EVMIQIKEL 483



>NP_703147
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           K+MIM++ +
Sbjct: 335 KLMIMLRSI 343



>NP_001152860
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           K+MIM++ +
Sbjct: 249 KLMIMLRSI 257



>NP_001152859
          Length = 506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           K+MIM++ +
Sbjct: 275 KLMIMLRSI 283



>NP_001003894
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           ++MI IKE+
Sbjct: 474 EVMIQIKEL 482



>NP_001001722
          Length = 541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KIMIMIKEV 9
           ++MI IKE+
Sbjct: 475 EVMIQIKEL 483



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.150    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 260,690
Number of extensions: 215
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)