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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
XP_006714622                                                           17    4312
XP_005271962                                                           17    4312
NP_065812                                                              16    7355
NP_714544                                                              15    9605
NP_057279                                                              15    9605
NP_001271188                                                           15    9605
NP_612417                                                              15    9605

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KILQIDSSI 9
            KILQIDSS+
Sbjct: 1651 KILQIDSSV 1659



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    KILQIDSSI 9
            KILQIDSS+
Sbjct: 1651 KILQIDSSV 1659



>XP_006714622
          Length = 266

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KILQIDSSI 9
          KIL+ DSSI
Sbjct: 30 KILRCDSSI 38



>XP_005271962
          Length = 266

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KILQIDSSI 9
          KIL+ DSSI
Sbjct: 30 KILRCDSSI 38



>NP_065812
          Length = 1335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KILQIDSS 8
           KIL +DSS
Sbjct: 497 KILAVDSS 504



>NP_714544
          Length = 1775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KILQIDS 7
            K+LQ+DS
Sbjct: 1141 KLLQLDS 1147



>NP_057279
          Length = 1780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KILQIDSS 8
           KILQI+ S
Sbjct: 358 KILQIEDS 365



>NP_001271188
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KILQIDSS 8
           KILQI+ S
Sbjct: 358 KILQIEDS 365



>NP_612417
          Length = 213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  ILQIDSSI 9
          ILQ+DS +
Sbjct: 54 ILQVDSCV 61



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.137    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 412,537
Number of extensions: 545
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)