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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036462                                                              17    2528
NP_001243397                                                           17    2528
XP_005269721                                                           17    2528
NP_055891                                                              16    5631
XP_005266929                                                           16    5631
XP_005266928                                                           16    5631
XP_005266927                                                           16    5631
XP_006715465                                                           16    5631
NP_000159                                                              16    7355
NP_001020279                                                           16    7355
NP_001640                                                              16    7355
NP_002276                                                              16    7355
XP_005274557                                                           16    7355
XP_005249761                                                           16    7355
XP_005249760                                                           16    7355
XP_005264002                                                           16    7355
XP_005264000                                                           16    7355
XP_006712586                                                           16    7355
XP_005263999                                                           16    7355
NP_005889                                                              16    7355
NP_976240                                                              16    7355
NP_004689                                                              15    9605
NP_710162                                                              15    9605
NP_001161842                                                           15    9605
NP_004738                                                              15    9605
XP_006725185                                                           15    9605
XP_006725184                                                           15    9605
XP_006725183                                                           15    9605
XP_006718119                                                           15    9605
XP_006718118                                                           15    9605
XP_005270333                                                           15    9605
XP_005249560                                                           15    9605

>NP_036462
          Length = 2073

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KHSSSRLAV 9
           K SSSRLAV
Sbjct: 393 KDSSSRLAV 401



>NP_001243397
          Length = 1890

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KHSSSRLAV 9
           K SSSRLAV
Sbjct: 393 KDSSSRLAV 401



>XP_005269721
          Length = 2073

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KHSSSRLAV 9
           K SSSRLAV
Sbjct: 393 KDSSSRLAV 401



>NP_055891
          Length = 502

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KHSSSRL 7
           +HSSSRL
Sbjct: 459 QHSSSRL 465



>XP_005266929
          Length = 329

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KHSSSRL 7
           +HSSSRL
Sbjct: 286 QHSSSRL 292



>XP_005266928
          Length = 378

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KHSSSRL 7
           +HSSSRL
Sbjct: 335 QHSSSRL 341



>XP_005266927
          Length = 418

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KHSSSRL 7
           +HSSSRL
Sbjct: 375 QHSSSRL 381



>XP_006715465
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KHSSSRL 7
           +HSSSRL
Sbjct: 399 QHSSSRL 405



>NP_000159
          Length = 1580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HSSSRL 7
            HSSSRL
Sbjct: 1533 HSSSRL 1538



>NP_001020279
          Length = 1251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KHSSSRLA 8
           K SSSRLA
Sbjct: 864 KDSSSRLA 871



>NP_001640
          Length = 1616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HSSSRLAV 9
           H SSRL+V
Sbjct: 183 HPSSRLSV 190



>NP_002276
          Length = 1226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KHSSSRLA 8
           K SSSRLA
Sbjct: 839 KDSSSRLA 846



>XP_005274557
          Length = 1615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HSSSRLAV 9
           H SSRL+V
Sbjct: 183 HPSSRLSV 190



>XP_005249761
          Length = 1580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HSSSRL 7
            HSSSRL
Sbjct: 1533 HSSSRL 1538



>XP_005249760
          Length = 1580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HSSSRL 7
            HSSSRL
Sbjct: 1533 HSSSRL 1538



>XP_005264002
          Length = 895

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KHSSSRLA 8
           K SSSRLA
Sbjct: 508 KDSSSRLA 515



>XP_005264000
          Length = 1226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KHSSSRLA 8
           K SSSRLA
Sbjct: 839 KDSSSRLA 846



>XP_006712586
          Length = 1262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KHSSSRLA 8
           K SSSRLA
Sbjct: 891 KDSSSRLA 898



>XP_005263999
          Length = 1278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KHSSSRLA 8
           K SSSRLA
Sbjct: 891 KDSSSRLA 898



>NP_005889
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HSSSRLA 8
           HS SRLA
Sbjct: 412 HSESRLA 418



>NP_976240
          Length = 694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HSSSRLA 8
           HS SRLA
Sbjct: 412 HSESRLA 418



>NP_004689
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   HSSSRLAV 9
           H+S+RLA+
Sbjct: 365 HTSTRLAL 372



>NP_710162
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KHSSSRL 7
           +HSSSR+
Sbjct: 161 EHSSSRI 167



>NP_001161842
          Length = 1782

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  KHSSSRLAV 9
          +H+ SRL+V
Sbjct: 34 RHAPSRLSV 42



>NP_004738
          Length = 1919

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1483 KQSSSRIA 1490



>XP_006725185
          Length = 1579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1143 KQSSSRIA 1150



>XP_006725184
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1471 KQSSSRIA 1478



>XP_006725183
          Length = 1915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1479 KQSSSRIA 1486



>XP_006718119
          Length = 1579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1143 KQSSSRIA 1150



>XP_006718118
          Length = 1907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1471 KQSSSRIA 1478



>XP_005270333
          Length = 1915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KHSSSRLA 8
            K SSSR+A
Sbjct: 1479 KQSSSRIA 1486



>XP_005249560
          Length = 1750

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  KHSSSRLAV 9
          +H+ SRL+V
Sbjct: 34 RHAPSRLSV 42



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.110    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 464,533
Number of extensions: 456
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)