Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           20     509
NP_000242                                                              20     509
XP_005264389                                                           20     509
NP_003782                                                              17    3301
NP_997211                                                              17    4312
NP_006640                                                              17    4312
XP_006723231                                                           17    4312
XP_005258877                                                           17    4312
XP_005258876                                                           17    4312
XP_006712378                                                           17    4312
XP_005263945                                                           17    4312
XP_006712373                                                           17    4312
XP_006712372                                                           17    4312
XP_005263939                                                           17    4312
NP_001159693                                                           17    4312
NP_067677                                                              17    4312
NP_054756                                                              17    4312
NP_003631                                                              16    5631
NP_001073867                                                           16    5631
XP_005252342                                                           16    5631
XP_005251911                                                           16    5631
XP_005251910                                                           16    5631
XP_005251909                                                           16    5631
XP_005251908                                                           16    5631
XP_005251907                                                           16    5631
NP_671709                                                              16    5631
NP_940980                                                              16    7355
NP_057427                                                              16    7355
XP_005268686                                                           16    7355
NP_060719                                                              15    9605
NP_001258968                                                           15    9605
NP_001011649                                                           15    9605
NP_001073953                                                           15    9605
NP_002102                                                              15    9605
NP_116207                                                              15    9605
NP_001138356                                                           15    9605
NP_598003                                                              15    9605
NP_219479                                                              15    9605
NP_060474                                                              15    9605
XP_006717248                                                           15    9605
XP_006717247                                                           15    9605
XP_006717246                                                           15    9605
XP_006717245                                                           15    9605
XP_006717244                                                           15    9605
XP_006717243                                                           15    9605
XP_006717242                                                           15    9605
XP_005248705                                                           15    9605
XP_006715387                                                           15    9605
XP_006715386                                                           15    9605
XP_005247626                                                           15    9605
XP_005247625                                                           15    9605
XP_006711061                                                           15    9605
XP_006711060                                                           15    9605
XP_005244868                                                           15    9605
XP_005270560                                                           15    9605
NP_859057                                                              15    9605
>NP_001245210
          Length = 868

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KHQKLLLVV 9
           KHQKLLL V
Sbjct: 361 KHQKLLLAV 369
>NP_000242
          Length = 934

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KHQKLLLVV 9
           KHQKLLL V
Sbjct: 427 KHQKLLLAV 435
>XP_005264389
          Length = 921

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KHQKLLLVV 9
           KHQKLLL V
Sbjct: 427 KHQKLLLAV 435
>NP_003782
          Length = 1052

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KHQKLLLV 8
           KHQKLL +
Sbjct: 948 KHQKLLSI 955
>NP_997211
          Length = 795

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           KHQKLL
Sbjct: 473 KHQKLL 478
>NP_006640
          Length = 771

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KHQKLL 6
          KHQKLL
Sbjct: 42 KHQKLL 47
>XP_006723231
          Length = 736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KHQKLLLV 8
           KH KLL+V
Sbjct: 533 KHTKLLIV 540
>XP_005258877
          Length = 736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KHQKLLLV 8
           KH KLL+V
Sbjct: 533 KHTKLLIV 540
>XP_005258876
          Length = 818

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KHQKLLLV 8
           KH KLL+V
Sbjct: 615 KHTKLLIV 622
>XP_006712378
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           KHQKLL
Sbjct: 473 KHQKLL 478
>XP_005263945
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           KHQKLL
Sbjct: 473 KHQKLL 478
>XP_006712373
          Length = 724

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           KHQKLL
Sbjct: 402 KHQKLL 407
>XP_006712372
          Length = 724

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           KHQKLL
Sbjct: 402 KHQKLL 407
>XP_005263939
          Length = 821

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           KHQKLL
Sbjct: 473 KHQKLL 478
>NP_001159693
          Length = 719

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KHQKLL 6
          KHQKLL
Sbjct: 42 KHQKLL 47
>NP_067677
          Length = 766

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KHQKLL 6
          KHQKLL
Sbjct: 41 KHQKLL 46
>NP_054756
          Length = 736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KHQKLLLV 8
           KH KLL+V
Sbjct: 533 KHTKLLIV 540
>NP_003631
          Length = 1332

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KHQKLLLVV 9
            +H+K LLVV
Sbjct: 1133 RHKKRLLVV 1141
>NP_001073867
          Length = 2017

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    HQKLLL 7
            HQKLLL
Sbjct: 1049 HQKLLL 1054
>XP_005252342
          Length = 1218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KHQKLLLVV 9
            +H+K LLVV
Sbjct: 1019 RHKKRLLVV 1027
>XP_005251911
          Length = 1839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKLLL
Sbjct: 871 HQKLLL 876
>XP_005251910
          Length = 1839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKLLL
Sbjct: 871 HQKLLL 876
>XP_005251909
          Length = 1839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKLLL
Sbjct: 871 HQKLLL 876
>XP_005251908
          Length = 1839

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKLLL
Sbjct: 871 HQKLLL 876
>XP_005251907
          Length = 1845

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKLLL
Sbjct: 877 HQKLLL 882
>NP_671709
          Length = 173

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   HQKLLLVV 9
           H+KL+L+V
Sbjct: 166 HKKLVLIV 173
>NP_940980
          Length = 2527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KHQKLLL 7
            +HQK+LL
Sbjct: 1821 EHQKILL 1827
>NP_057427
          Length = 3114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    KHQKLLL 7
            KHQ LLL
Sbjct: 2709 KHQALLL 2715
>XP_005268686
          Length = 2521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    KHQKLLL 7
            +HQK+LL
Sbjct: 1821 EHQKILL 1827
>NP_060719
          Length = 1893

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 986 HQKLIL 991
>NP_001258968
          Length = 1663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 756 HQKLIL 761
>NP_001011649
          Length = 1814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 986 HQKLIL 991
>NP_001073953
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           +HQKLL
Sbjct: 237 RHQKLL 242
>NP_002102
          Length = 3144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HQKLLLVV 9
            H+KLLL +
Sbjct: 2596 HEKLLLQI 2603
>NP_116207
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HQKLLLV 8
           H+KLLL+
Sbjct: 306 HEKLLLM 312
>NP_001138356
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HQKLLLV 8
           H++LLLV
Sbjct: 138 HERLLLV 144
>NP_598003
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HQKLLLV 8
           H++LLLV
Sbjct: 138 HERLLLV 144
>NP_219479
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KHQKLLL 7
           +HQ+LLL
Sbjct: 96  RHQQLLL 102
>NP_060474
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HQKLLLVV 9
           HQ L+LVV
Sbjct: 153 HQLLVLVV 160
>XP_006717248
          Length = 1664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 757 HQKLIL 762
>XP_006717247
          Length = 1670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 757 HQKLIL 762
>XP_006717246
          Length = 1820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 986 HQKLIL 991
>XP_006717245
          Length = 1861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 954 HQKLIL 959
>XP_006717244
          Length = 1867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 954 HQKLIL 959
>XP_006717243
          Length = 1889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 976 HQKLIL 981
>XP_006717242
          Length = 1898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HQKLLL 7
           HQKL+L
Sbjct: 985 HQKLIL 990
>XP_005248705
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  KHQKLLL 7
          +HQ+LLL
Sbjct: 21 RHQQLLL 27
>XP_006715387
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  KHQKLLL 7
          +HQ+LLL
Sbjct: 30 RHQQLLL 36
>XP_006715386
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  KHQKLLL 7
          +HQ+LLL
Sbjct: 30 RHQQLLL 36
>XP_005247626
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HQKLLLVV 9
           HQ L+LVV
Sbjct: 169 HQLLVLVV 176
>XP_005247625
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HQKLLLVV 9
           HQ L+LVV
Sbjct: 169 HQLLVLVV 176
>XP_006711061
          Length = 1553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           +HQKLL
Sbjct: 151 RHQKLL 156
>XP_006711060
          Length = 1595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           +HQKLL
Sbjct: 193 RHQKLL 198
>XP_005244868
          Length = 1639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KHQKLL 6
           +HQKLL
Sbjct: 237 RHQKLL 242
>XP_005270560
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HQKLLLV 8
           H++LLLV
Sbjct: 138 HERLLLV 144
>NP_859057
          Length = 149

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  KHQKLLLV 8
          K+QKL+L+
Sbjct: 30 KYQKLILM 37
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.144    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 561,167
Number of extensions: 776
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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