Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           21     175
NP_001257437                                                           21     175
NP_006281                                                              21     175
XP_006715618                                                           21     175
XP_005267176                                                           21     175
NP_689993                                                              17    4312
NP_001161939                                                           17    4312
NP_001161938                                                           17    4312
NP_001161937                                                           17    4312
XP_005274537                                                           17    4312
XP_005274536                                                           17    4312
XP_005274535                                                           17    4312
XP_006724545                                                           17    4312
XP_006724544                                                           17    4312
NP_001157951                                                           16    5631
NP_443076                                                              16    5631
NP_001257449                                                           16    5631
NP_055807                                                              16    5631
NP_690051                                                              16    5631
NP_006038                                                              16    5631
NP_001185769                                                           16    5631
NP_001185767                                                           16    5631
NP_078945                                                              16    5631
XP_005257785                                                           16    5631
XP_005267488                                                           16    5631
XP_005267487                                                           16    5631
XP_005250515                                                           16    5631
XP_005250514                                                           16    5631
XP_006714754                                                           16    5631
XP_005248656                                                           16    5631
NP_061877                                                              16    5631
NP_063945                                                              16    7355
NP_878315                                                              16    7355
NP_699165                                                              16    7355
NP_077808                                                              16    7355
NP_006573                                                              16    7355
NP_001269952                                                           16    7355
NP_064502                                                              16    7355
XP_006717250                                                           16    7355
NP_000279                                                              15    9605
NP_001186732                                                           15    9605
NP_077298                                                              15    9605
NP_003049                                                              15    9605
NP_777612                                                              15    9605
NP_005384                                                              15    9605
NP_061146                                                              15    9605
NP_001005739                                                           15    9605
NP_001156729                                                           15    9605
NP_954872                                                              15    9605
NP_057600                                                              15    9605
XP_005267076                                                           15    9605
XP_006712165                                                           15    9605
XP_006712092                                                           15    9605
>NP_001257436
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KHFASASGK 9
           KHFA+ASGK
Sbjct: 635 KHFAAASGK 643
>NP_001257437
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KHFASASGK 9
           KHFA+ASGK
Sbjct: 635 KHFAAASGK 643
>NP_006281
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KHFASASGK 9
           KHFA+ASGK
Sbjct: 635 KHFAAASGK 643
>XP_006715618
          Length = 577

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KHFASASGK 9
           KHFA+ASGK
Sbjct: 422 KHFAAASGK 430
>XP_005267176
          Length = 790

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KHFASASGK 9
           KHFA+ASGK
Sbjct: 635 KHFAAASGK 643
>NP_689993
          Length = 732

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 372 KHAAAAGGK 380
>NP_001161939
          Length = 680

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 320 KHAAAAGGK 328
>NP_001161938
          Length = 687

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 327 KHAAAAGGK 335
>NP_001161937
          Length = 773

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 413 KHAAAAGGK 421
>XP_005274537
          Length = 724

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 364 KHAAAAGGK 372
>XP_005274536
          Length = 746

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 386 KHAAAAGGK 394
>XP_005274535
          Length = 757

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 397 KHAAAAGGK 405
>XP_006724545
          Length = 765

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 405 KHAAAAGGK 413
>XP_006724544
          Length = 806

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KH A+A GK
Sbjct: 446 KHAAAAGGK 454
>NP_001157951
          Length = 138

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  HFASASGK 9
          H AS SGK
Sbjct: 81 HMASQSGK 88
>NP_443076
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FASASGK 9
           FA+ASGK
Sbjct: 451 FAAASGK 457
>NP_001257449
          Length = 1068

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KHF   +GK
Sbjct: 948 KHFGEEAGK 956
>NP_055807
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KHF   +GK
Sbjct: 958 KHFGEEAGK 966
>NP_690051
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FASASGK 9
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_006038
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FASASGK 9
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_001185769
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FASASGK 9
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_001185767
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FASASGK 9
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_078945
          Length = 614

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   HFASASGK 9
           H+A+A+GK
Sbjct: 270 HWAAAAGK 277
>XP_005257785
          Length = 1887

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KHFASASG 8
           +HF+ ASG
Sbjct: 132 QHFSQASG 139
>XP_005267488
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KHF   +GK
Sbjct: 958 KHFGEEAGK 966
>XP_005267487
          Length = 1078

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KHF   +GK
Sbjct: 958 KHFGEEAGK 966
>XP_005250515
          Length = 1083

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HFASASG 8
           H+A+ASG
Sbjct: 150 HYAAASG 156
>XP_005250514
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HFASASG 8
           H+A+ASG
Sbjct: 150 HYAAASG 156
>XP_006714754
          Length = 452

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   HFASASGK 9
           H+A+A+GK
Sbjct: 108 HWAAAAGK 115
>XP_005248656
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   HFASASGK 9
           H+A+A+GK
Sbjct: 147 HWAAAAGK 154
>NP_061877
          Length = 1089

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   HFASASG 8
           H+A+ASG
Sbjct: 150 HYAAASG 156
>NP_063945
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   HFASASGK 9
           HF S  GK
Sbjct: 424 HFTSGDGK 431
>NP_878315
          Length = 866

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KHFASASGK 9
           KH A+A+G+
Sbjct: 671 KHSAAAAGR 679
>NP_699165
          Length = 871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KHFASASGK 9
           KH A+A+G+
Sbjct: 676 KHSAAAAGR 684
>NP_077808
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KHF   +GK
Sbjct: 121 KHFVHLAGK 129
>NP_006573
          Length = 573

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KHF   +GK
Sbjct: 131 KHFVHLAGK 139
>NP_001269952
          Length = 320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  KHFASASG 8
          KH A++SG
Sbjct: 69 KHLAASSG 76
>NP_064502
          Length = 1176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KHFASASGK 9
           KHF + +GK
Sbjct: 957 KHFEANNGK 965
>XP_006717250
          Length = 610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   HFASASGK 9
           HF S  GK
Sbjct: 390 HFTSGDGK 397
>NP_000279
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FASASG 8
           FASASG
Sbjct: 171 FASASG 176
>NP_001186732
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   HFASASGK 9
           HF   SGK
Sbjct: 103 HFTGLSGK 110
>NP_077298
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   HFASASGK 9
           HF   SGK
Sbjct: 109 HFTGLSGK 116
>NP_003049
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KHFASASGK 9
           KH A  +GK
Sbjct: 304 KHIAKKNGK 312
>NP_777612
          Length = 459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   HFASASGK 9
           HF ++SG+
Sbjct: 142 HFGNSSGR 149
>NP_005384
          Length = 1909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    FASASG 8
            FASASG
Sbjct: 1133 FASASG 1138
>NP_061146
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   KHFASASG 8
           +HF  ASG
Sbjct: 710 EHFQEASG 717
>NP_001005739
          Length = 965

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HFASASGK 9
           HF +A GK
Sbjct: 175 HFNTAGGK 182
>NP_001156729
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    FASASG 8
            FASASG
Sbjct: 1156 FASASG 1161
>NP_954872
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   KHFASASG 8
           +HF  ASG
Sbjct: 657 EHFQEASG 664
>NP_057600
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HFASASGK 9
           HF +A GK
Sbjct: 187 HFNTAGGK 194
>XP_005267076
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FASASG 8
           FASASG
Sbjct: 133 FASASG 138
>XP_006712165
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   HFASASGK 9
           HF   SGK
Sbjct: 109 HFTGLSGK 116
>XP_006712092
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HFASASGK 9
           HF +A GK
Sbjct: 187 HFNTAGGK 194
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.115    0.322 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 525,209
Number of extensions: 1020
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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