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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              21     175
NP_001129166                                                           17    3301
NP_005653                                                              17    3301
NP_055455                                                              17    3301
XP_006712938                                                           17    3301
XP_006712937                                                           17    3301
XP_006712936                                                           17    3301
XP_005247034                                                           17    3301
NP_733759                                                              17    4312
NP_055558                                                              16    5631
NP_001094060                                                           16    5631
NP_001181966                                                           16    5631
NP_001071174                                                           16    5631
XP_006715535                                                           16    5631
XP_005248098                                                           16    5631
XP_006713998                                                           16    5631
XP_006713997                                                           16    5631
NP_000432                                                              16    7355
NP_002086                                                              16    7355
NP_001159530                                                           16    7355
NP_570718                                                              16    7355
XP_006716088                                                           16    7355
XP_006716087                                                           16    7355
XP_005250482                                                           16    7355
NP_660159                                                              15    9605
NP_003366                                                              15    9605
NP_001171752                                                           15    9605
NP_064505                                                              15    9605
NP_001171712                                                           15    9605
NP_056280                                                              15    9605
NP_001239085                                                           15    9605
XP_006718024                                                           15    9605
XP_006715412                                                           15    9605
XP_006712699                                                           15    9605
XP_006712698                                                           15    9605
XP_006712697                                                           15    9605

>NP_002430
          Length = 1137

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KGYKMGVVK 9
           KGYK+GVVK
Sbjct: 300 KGYKVGVVK 308



>NP_001129166
          Length = 284

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KGY  G+VK
Sbjct: 20 KGYGFGMVK 28



>NP_005653
          Length = 283

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KGY  G+VK
Sbjct: 20 KGYGFGMVK 28



>NP_055455
          Length = 1199

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KGYKMGVV 8
            KG+ MGVV
Sbjct: 1073 KGFHMGVV 1080



>XP_006712938
          Length = 1138

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KGYKMGVV 8
            KG+ MGVV
Sbjct: 1073 KGFHMGVV 1080



>XP_006712937
          Length = 1199

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KGYKMGVV 8
            KG+ MGVV
Sbjct: 1073 KGFHMGVV 1080



>XP_006712936
          Length = 1199

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KGYKMGVV 8
            KG+ MGVV
Sbjct: 1073 KGFHMGVV 1080



>XP_005247034
          Length = 1199

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KGYKMGVV 8
            KG+ MGVV
Sbjct: 1073 KGFHMGVV 1080



>NP_733759
          Length = 127

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KG+K  VVK
Sbjct: 14 KGFKKAVVK 22



>NP_055558
          Length = 1395

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GYKMGVVK 9
           GY  GV+K
Sbjct: 819 GYSSGVIK 826



>NP_001094060
          Length = 1395

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GYKMGVVK 9
           GY  GV+K
Sbjct: 819 GYSSGVIK 826



>NP_001181966
          Length = 904

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KGYKMGV 7
           +GYK+GV
Sbjct: 356 QGYKIGV 362



>NP_001071174
          Length = 904

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KGYKMGV 7
           +GYK+GV
Sbjct: 356 QGYKIGV 362



>XP_006715535
          Length = 946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KGYKMGV 7
           +GYK+GV
Sbjct: 356 QGYKIGV 362



>XP_005248098
          Length = 1310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GYKMGVVK 9
           GY  GV+K
Sbjct: 710 GYSSGVIK 717



>XP_006713998
          Length = 1332

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GYKMGVVK 9
           GY  GV+K
Sbjct: 819 GYSSGVIK 826



>XP_006713997
          Length = 1419

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   GYKMGVVK 9
           GY  GV+K
Sbjct: 819 GYSSGVIK 826



>NP_000432
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KGYKMGVVK 9
           K Y  G+VK
Sbjct: 321 KNYNAGIVK 329



>NP_002086
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GYKMGVV 8
          GYK GV+
Sbjct: 72 GYKFGVL 78



>NP_001159530
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   KGYKMGV 7
           KGY +G+
Sbjct: 563 KGYSLGI 569



>NP_570718
          Length = 754

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   KGYKMGV 7
           KGY +G+
Sbjct: 587 KGYSLGI 593



>XP_006716088
          Length = 423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KGYKMGVVK 9
           K Y  G+VK
Sbjct: 321 KNYNAGIVK 329



>XP_006716087
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KGYKMGVVK 9
           K Y  G+VK
Sbjct: 321 KNYNAGIVK 329



>XP_005250482
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   KGYKMGVVK 9
           K Y  G+VK
Sbjct: 321 KNYNAGIVK 329



>NP_660159
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 2  GYKMGVV 8
          GY++G++
Sbjct: 55 GYELGII 61



>NP_003366
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KG+  G+VK
Sbjct: 31 KGFGFGLVK 39



>NP_001171752
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KG+  G+VK
Sbjct: 31 KGFGFGLVK 39



>NP_064505
          Length = 1555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  YKMGVV 8
          YKMGV+
Sbjct: 23 YKMGVL 28



>NP_001171712
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KG+  G+VK
Sbjct: 46 KGFGFGLVK 54



>NP_056280
          Length = 1508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GYKMGVV 8
           GYK G+V
Sbjct: 170 GYKDGIV 176



>NP_001239085
          Length = 1507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GYKMGVV 8
           GYK G+V
Sbjct: 170 GYKDGIV 176



>XP_006718024
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  KGYKMGVVK 9
          KG+  G+VK
Sbjct: 31 KGFGFGLVK 39



>XP_006715412
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/7 (42%), Positives = 7/7 (100%)

Query: 2  GYKMGVV 8
          GY++G++
Sbjct: 55 GYELGII 61



>XP_006712699
          Length = 1544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  YKMGVV 8
          YKMGV+
Sbjct: 12 YKMGVL 17



>XP_006712698
          Length = 1547

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  YKMGVV 8
          YKMGV+
Sbjct: 23 YKMGVL 28



>XP_006712697
          Length = 1549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  YKMGVV 8
          YKMGV+
Sbjct: 17 YKMGVL 22



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.141    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 484,079
Number of extensions: 648
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)