Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_849161                                                              17    4312
NP_037506                                                              16    7355
NP_001155901                                                           16    7355
NP_001243121                                                           16    7355
NP_002200                                                              16    7355
NP_006794                                                              16    7355
NP_001127768                                                           16    7355
NP_056379                                                              16    7355
XP_006726821                                                           16    7355
XP_006726820                                                           16    7355
XP_006726819                                                           16    7355
XP_006726818                                                           16    7355
XP_006726817                                                           16    7355
XP_006726816                                                           16    7355
XP_006726815                                                           16    7355
XP_006726814                                                           16    7355
XP_006726813                                                           16    7355
XP_006726812                                                           16    7355
XP_006726811                                                           16    7355
XP_006726810                                                           16    7355
XP_006722325                                                           16    7355
XP_005255370                                                           16    7355
XP_006716339                                                           16    7355
XP_006710099                                                           16    7355
NP_002606                                                              15    9605
NP_055851                                                              15    9605
NP_001245240                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KGTMSLIFL 9
            KGTMSL FL
Sbjct: 2940 KGTMSLTFL 2948
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KGTMSLIFL 9
            KGTMSL FL
Sbjct: 2735 KGTMSLTFL 2743
>NP_849161
          Length = 522

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GTMSLIF 8
           GTM+LIF
Sbjct: 431 GTMALIF 437
>NP_037506
          Length = 868

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GTMSLIFL 9
           GT+SLI L
Sbjct: 185 GTLSLIML 192
>NP_001155901
          Length = 873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GTMSLIFL 9
           GT+SLI L
Sbjct: 185 GTLSLIML 192
>NP_001243121
          Length = 271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GTMSLIFL 9
           GT+SLI L
Sbjct: 185 GTLSLIML 192
>NP_002200
          Length = 1170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KGTMSLIFL 9
           KG + L+FL
Sbjct: 152 KGNVDLVFL 160
>NP_006794
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 355 KGTMSL 360
>NP_001127768
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 355 KGTMSL 360
>NP_056379
          Length = 516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GTMSLIF 8
           GTM+L+F
Sbjct: 426 GTMALLF 432
>XP_006726821
          Length = 408

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006726820
          Length = 415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 285 KGTMSL 290
>XP_006726819
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 285 KGTMSL 290
>XP_006726818
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 263 KGTMSL 268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 369 KGTMSL 374
>XP_006726817
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 157 KGTMSL 162

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 369 KGTMSL 374
>XP_006726816
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006726815
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 263 KGTMSL 268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 369 KGTMSL 374
>XP_006726814
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 285 KGTMSL 290
>XP_006726813
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 285 KGTMSL 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 391 KGTMSL 396
>XP_006726812
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 285 KGTMSL 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 391 KGTMSL 396
>XP_006726811
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006726810
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006722325
          Length = 408

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_005255370
          Length = 1169

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KGTMSLIFL 9
           KG + L+FL
Sbjct: 152 KGNVDLVFL 160
>XP_006716339
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 355 KGTMSL 360
>XP_006710099
          Length = 441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KGTMSL 6
           KGTMSL
Sbjct: 210 KGTMSL 215
>NP_002606
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GTMSLIF 8
           G+MS+IF
Sbjct: 270 GSMSIIF 276
>NP_055851
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GTMSLIF 8
           GT+SL+F
Sbjct: 461 GTVSLLF 467
>NP_001245240
          Length = 524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KGTMSLI 7
           KG MSLI
Sbjct: 398 KGNMSLI 404
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.335    0.148    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,018
Number of extensions: 559
Number of successful extensions: 34
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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