Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1135
NP_036347                                                              17    3301
NP_001269973                                                           17    4312
NP_055805                                                              17    4312
NP_001269972                                                           17    4312
NP_919277                                                              17    4312
XP_005267555                                                           17    4312
XP_006720166                                                           17    4312
XP_005267550                                                           17    4312
XP_005267549                                                           17    4312
XP_005267548                                                           17    4312
XP_006720165                                                           17    4312
XP_006720164                                                           17    4312
XP_006720163                                                           17    4312
XP_006720162                                                           17    4312
XP_006720161                                                           17    4312
NP_001123292                                                           16    5631
XP_005258025                                                           16    5631
NP_001164224                                                           16    7355
NP_659454                                                              16    7355
NP_057079                                                              16    7355
NP_113626                                                              15    9605
NP_115803                                                              15    9605
NP_116038                                                              15    9605
XP_006716433                                                           15    9605
XP_005273633                                                           15    9605
XP_006716432                                                           15    9605
XP_005273632                                                           15    9605
XP_005272154                                                           15    9605
XP_005272153                                                           15    9605
XP_005272152                                                           15    9605
NP_112561                                                              15    9605
>NP_004251
          Length = 1208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    KFSSRAVA 8
            KFSSRAVA
Sbjct: 1154 KFSSRAVA 1161
>NP_036347
          Length = 916

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KFSSRAVAH 9
           ++SSR VAH
Sbjct: 396 QYSSRQVAH 404
>NP_001269973
          Length = 2083

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1373 KFTNKTVAH 1381
>NP_055805
          Length = 2036

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1326 KFTNKTVAH 1334
>NP_001269972
          Length = 2049

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1339 KFTNKTVAH 1347
>NP_919277
          Length = 2083

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1326 KFTNKTVAH 1334
>XP_005267555
          Length = 1325

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KFSSRAVAH 9
           KF+++ VAH
Sbjct: 567 KFTNKTVAH 575
>XP_006720166
          Length = 2083

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1373 KFTNKTVAH 1381
>XP_005267550
          Length = 2084

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1326 KFTNKTVAH 1334
>XP_005267549
          Length = 2130

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1373 KFTNKTVAH 1381
>XP_005267548
          Length = 2131

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1373 KFTNKTVAH 1381
>XP_006720165
          Length = 2542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1832 KFTNKTVAH 1840
>XP_006720164
          Length = 2542

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1832 KFTNKTVAH 1840
>XP_006720163
          Length = 2543

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1785 KFTNKTVAH 1793
>XP_006720162
          Length = 2589

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1832 KFTNKTVAH 1840
>XP_006720161
          Length = 2590

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KFSSRAVAH 9
            KF+++ VAH
Sbjct: 1832 KFTNKTVAH 1840
>NP_001123292
          Length = 912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FSSRAVAH 9
           F+SRA+ H
Sbjct: 894 FASRAIKH 901
>XP_005258025
          Length = 912

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   FSSRAVAH 9
           F+SRA+ H
Sbjct: 894 FASRAIKH 901
>NP_001164224
          Length = 405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSRAVAH 9
           SSR VAH
Sbjct: 398 SSRCVAH 404
>NP_659454
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   SSRAVAH 9
           SSR VAH
Sbjct: 418 SSRCVAH 424
>NP_057079
          Length = 288

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  SSRAVAH 9
          SSRAV H
Sbjct: 19 SSRAVVH 25
>NP_113626
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SRAVAH 9
           SRA+AH
Sbjct: 444 SRAIAH 449
>NP_115803
          Length = 1156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    KFSSRAVA 8
            +FS RAVA
Sbjct: 1008 QFSQRAVA 1015
>NP_116038
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   KFSSRAVAH 9
           KFS R V H
Sbjct: 372 KFSIRLVPH 380
>XP_006716433
          Length = 3069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KFSSRAVAH 9
            KF+S  +AH
Sbjct: 2255 KFNSNLLAH 2263
>XP_005273633
          Length = 3069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KFSSRAVAH 9
            KF+S  +AH
Sbjct: 2255 KFNSNLLAH 2263
>XP_006716432
          Length = 3172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KFSSRAVAH 9
            KF+S  +AH
Sbjct: 2358 KFNSNLLAH 2366
>XP_005273632
          Length = 3172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KFSSRAVAH 9
            KF+S  +AH
Sbjct: 2358 KFNSNLLAH 2366
>XP_005272154
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SRAVAH 9
           SRA+AH
Sbjct: 426 SRAIAH 431
>XP_005272153
          Length = 444

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SRAVAH 9
           SRA+AH
Sbjct: 426 SRAIAH 431
>XP_005272152
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SRAVAH 9
           SRA+AH
Sbjct: 444 SRAIAH 449
>NP_112561
          Length = 2789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KFSSRAVAH 9
            KF+S  +AH
Sbjct: 1975 KFNSNLLAH 1983
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.118    0.323 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 309,324
Number of extensions: 413
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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