Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001239155                                                           17    3301
NP_006613                                                              17    3301
NP_060720                                                              17    3301
NP_054868                                                              17    3301
XP_005273625                                                           17    3301
XP_006716420                                                           17    3301
XP_006716419                                                           17    3301
XP_005250342                                                           17    3301
NP_001138631                                                           17    3301
NP_001026862                                                           17    4312
NP_005815                                                              17    4312
XP_005250165                                                           17    4312
XP_006724713                                                           16    5631
XP_006724712                                                           16    5631
XP_006724711                                                           16    5631
NP_001155963                                                           16    5631
XP_006726471                                                           16    7355
XP_006710212                                                           16    7355
NP_001185708                                                           15    9605
NP_003582                                                              15    9605
NP_001185707                                                           15    9605
NP_001185706                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KFKLTTLLL 9
           +FKLTTLLL
Sbjct: 753 EFKLTTLLL 761
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KFKLTTLLL 9
           +FKLTTLLL
Sbjct: 548 EFKLTTLLL 556
>NP_001239155
          Length = 671

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FKLTTLLL 9
           F+LTTLL+
Sbjct: 641 FRLTTLLM 648
>NP_006613
          Length = 685

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   FKLTTLLL 9
           F+LTTLL+
Sbjct: 655 FRLTTLLM 662
>NP_060720
          Length = 658

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          KFK TT++L
Sbjct: 18 KFKSTTIML 26
>NP_054868
          Length = 747

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KFKLTTLL 8
           K KLTTLL
Sbjct: 736 KIKLTTLL 743
>XP_005273625
          Length = 427

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          KFK TT++L
Sbjct: 18 KFKSTTIML 26
>XP_006716420
          Length = 646

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          KFK TT++L
Sbjct: 18 KFKSTTIML 26
>XP_006716419
          Length = 676

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          KFK TT++L
Sbjct: 18 KFKSTTIML 26
>XP_005250342
          Length = 773

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KFKLTTLL 8
           K KLTTLL
Sbjct: 762 KIKLTTLL 769
>NP_001138631
          Length = 637

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          KFK TT++L
Sbjct: 18 KFKSTTIML 26
>NP_001026862
          Length = 441

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   KLTTLLL 9
           KLTTLLL
Sbjct: 130 KLTTLLL 136
>NP_005815
          Length = 313

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   KLTTLLL 9
           KLTTLLL
Sbjct: 130 KLTTLLL 136
>XP_005250165
          Length = 441

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   KLTTLLL 9
           KLTTLLL
Sbjct: 130 KLTTLLL 136
>XP_006724713
          Length = 238

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          K +LTTLLL
Sbjct: 2  KSELTTLLL 10
>XP_006724712
          Length = 250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          K +LTTLLL
Sbjct: 54 KSELTTLLL 62
>XP_006724711
          Length = 290

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          K +LTTLLL
Sbjct: 54 KSELTTLLL 62
>NP_001155963
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  KFKLTTLLL 9
          K +LTTLLL
Sbjct: 54 KSELTTLLL 62
>XP_006726471
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  KFKLTTLL 8
          +FK++TLL
Sbjct: 15 RFKISTLL 22
>XP_006710212
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  KFKLTTLL 8
          +FK++TLL
Sbjct: 15 RFKISTLL 22
>NP_001185708
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KFKLTT 6
           KFK+TT
Sbjct: 663 KFKITT 668
>NP_003582
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KFKLTT 6
           KFK+TT
Sbjct: 650 KFKITT 655
>NP_001185707
          Length = 764

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KFKLTT 6
           KFK+TT
Sbjct: 669 KFKITT 674
>NP_001185706
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   KFKLTT 6
           KFK+TT
Sbjct: 650 KFKITT 655
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.149    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 312,922
Number of extensions: 356
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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