Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001121619                                                           19     665
NP_000258                                                              19     665
NP_001035957                                                           19     665
XP_006721991                                                           19     665
XP_006721990                                                           19     665
XP_006721989                                                           19     665
XP_005258041                                                           19     665
XP_006721988                                                           19     665
XP_006721987                                                           19     665
XP_006721986                                                           19     665
XP_005258040                                                           19     665
XP_006721985                                                           19     665
NP_060606                                                              17    3301
NP_071504                                                              16    5631
NP_000911                                                              16    5631
NP_001035806                                                           16    5631
NP_008987                                                              16    5631
NP_001265368                                                           16    5631
XP_006723054                                                           16    5631
XP_006718642                                                           16    5631
XP_006718641                                                           16    5631
XP_005274089                                                           16    5631
XP_005274088                                                           16    5631
XP_006718640                                                           16    5631
NP_001166039                                                           16    7355
NP_002459                                                              16    7355
NP_001166038                                                           16    7355
NP_055952                                                              16    7355
NP_005862                                                              16    7355
NP_065772                                                              16    7355
XP_006724837                                                           16    7355
XP_005269439                                                           16    7355
XP_005250906                                                           16    7355
XP_005246783                                                           16    7355
NP_065994                                                              16    7355
NP_000676                                                              15    9605
NP_033611                                                              15    9605
NP_114438                                                              15    9605
NP_004826                                                              15    9605
NP_114038                                                              15    9605
NP_001010986                                                           15    9605
NP_775965                                                              15    9605
NP_056020                                                              15    9605
NP_115565                                                              15    9605
NP_002087                                                              15    9605
XP_006724816                                                           15    9605
XP_006724815                                                           15    9605
XP_006724814                                                           15    9605
XP_005262462                                                           15    9605
XP_006720028                                                           15    9605
XP_005268363                                                           15    9605
XP_005268362                                                           15    9605
XP_005268358                                                           15    9605
XP_005268360                                                           15    9605
XP_005268357                                                           15    9605
XP_005268356                                                           15    9605
>NP_001121619
          Length = 593

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>NP_000258
          Length = 2818

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>NP_001035957
          Length = 2839

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721991
          Length = 1632

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721990
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721989
          Length = 2823

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_005258041
          Length = 2836

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721988
          Length = 2846

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721987
          Length = 2849

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721986
          Length = 2854

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 187 KFKALKKVA 195
>XP_005258040
          Length = 2857

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>XP_006721985
          Length = 2867

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           KFKALKK A
Sbjct: 200 KFKALKKVA 208
>NP_060606
          Length = 3477

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KFKALKKAA 9
            K+K +KKAA
Sbjct: 1601 KYKKMKKAA 1609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    KFKALKKAA 9
            K+K +KKAA
Sbjct: 1989 KWKIMKKAA 1997
>NP_071504
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>NP_000911
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>NP_001035806
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>NP_008987
          Length = 674

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           +FKA KKAA
Sbjct: 589 QFKADKKAA 597
>NP_001265368
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           +FKA KKAA
Sbjct: 527 QFKADKKAA 535
>XP_006723054
          Length = 445

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   KFKALKKAA 9
           +FKA KKAA
Sbjct: 360 QFKADKKAA 368
>XP_006718642
          Length = 671

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 379 KFKEVKKA 386
>XP_006718641
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>XP_005274089
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>XP_005274088
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>XP_006718640
          Length = 1178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           KFK +KKA
Sbjct: 886 KFKEVKKA 893
>NP_001166039
          Length = 264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KFKALKK 7
           K+KA+KK
Sbjct: 224 KYKAMKK 230
>NP_002459
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KFKALKK 7
           K+KA+KK
Sbjct: 269 KYKAMKK 275
>NP_001166038
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KFKALKK 7
           K+KA+KK
Sbjct: 277 KYKAMKK 283
>NP_055952
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           F+ALKKA
Sbjct: 728 FEALKKA 734
>NP_005862
          Length = 238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           K KALKKA
Sbjct: 154 KKKALKKA 161
>NP_065772
          Length = 1709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   KFKALK 6
           KFKALK
Sbjct: 166 KFKALK 171
>XP_006724837
          Length = 1533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KFKALK 6
          KFKALK
Sbjct: 26 KFKALK 31
>XP_005269439
          Length = 199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   KFKALKKA 8
           K KALKKA
Sbjct: 115 KKKALKKA 122
>XP_005250906
          Length = 785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           F+ALKKA
Sbjct: 692 FEALKKA 698
>XP_005246783
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KFKALKK 7
           K+KA+KK
Sbjct: 397 KYKAIKK 403
>NP_065994
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KFKALKK 7
           K+KA+KK
Sbjct: 397 KYKAIKK 403
>NP_000676
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           +KALKKA
Sbjct: 219 WKALKKA 225
>NP_033611
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           +KALKKA
Sbjct: 219 WKALKKA 225
>NP_114438
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           +KALKKA
Sbjct: 248 WKALKKA 254
>NP_004826
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           +KALKKA
Sbjct: 254 WKALKKA 260
>NP_114038
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   FKALKKA 8
           +KALKKA
Sbjct: 254 WKALKKA 260
>NP_001010986
          Length = 1119

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 854 KFKHLKK 860
>NP_775965
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 854 KFKHLKK 860
>NP_056020
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>NP_115565
          Length = 1191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>NP_002087
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK +KK
Sbjct: 109 KFKGIKK 115
>XP_006724816
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 851 KFKHLKK 857
>XP_006724815
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 851 KFKHLKK 857
>XP_006724814
          Length = 1116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 851 KFKHLKK 857
>XP_005262462
          Length = 1129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 851 KFKHLKK 857
>XP_006720028
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>XP_005268363
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>XP_005268362
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>XP_005268358
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>XP_005268360
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>XP_005268357
          Length = 1171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
>XP_005268356
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   KFKALKK 7
           KFK LKK
Sbjct: 860 KFKHLKK 866
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.123    0.300 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 313,927
Number of extensions: 601
Number of successful extensions: 57
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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