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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1482
NP_694551                                                              18    1482
XP_006716142                                                           18    1482
XP_005250088                                                           18    1482
XP_006716141                                                           18    1482
XP_005250085                                                           18    1482
XP_006716140                                                           18    1482
XP_005250084                                                           18    1482
XP_005250083                                                           18    1482
XP_005250082                                                           18    1482
NP_001122099                                                           16    5631
NP_001122098                                                           16    5631
NP_064533                                                              16    5631
XP_006722397                                                           16    5631
XP_005258179                                                           16    5631
XP_005258178                                                           16    5631
XP_006722396                                                           16    5631

>NP_733751
          Length = 4911

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4867 KIIISSSRR 4875



>NP_694551
          Length = 534

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   KFIISSSRR 9
           KF++SSSR+
Sbjct: 444 KFVVSSSRQ 452



>XP_006716142
          Length = 4927

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4883 KIIISSSRR 4891



>XP_005250088
          Length = 4928

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4884 KIIISSSRR 4892



>XP_006716141
          Length = 4959

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4915 KIIISSSRR 4923



>XP_005250085
          Length = 4979

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4935 KIIISSSRR 4943



>XP_006716140
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4938 KIIISSSRR 4946



>XP_005250084
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4938 KIIISSSRR 4946



>XP_005250083
          Length = 4982

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4938 KIIISSSRR 4946



>XP_005250082
          Length = 4983

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    KFIISSSRR 9
            K IISSSRR
Sbjct: 4939 KIIISSSRR 4947



>NP_001122099
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 578 IISSSRR 584



>NP_001122098
          Length = 756

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 712 IISSSRR 718



>NP_064533
          Length = 742

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 698 IISSSRR 704



>XP_006722397
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 368 IISSSRR 374



>XP_005258179
          Length = 800

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 756 IISSSRR 762



>XP_005258178
          Length = 692

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 648 IISSSRR 654



>XP_006722396
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IISSSRR 9
           IISSSRR
Sbjct: 701 IISSSRR 707



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.136    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 332,930
Number of extensions: 301
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)