Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001120972                                                           19     869
XP_006716051                                                           19     869
NP_932765                                                              17    4312
NP_056437                                                              17    4312
NP_783161                                                              16    7355
NP_056029                                                              16    7355
NP_001157704                                                           16    7355
XP_005273513                                                           16    7355
XP_005273511                                                           16    7355
XP_005273512                                                           16    7355
XP_006715921                                                           16    7355
XP_005250007                                                           16    7355
NP_001177645                                                           15    9605
NP_001254651                                                           15    9605
NP_008878                                                              15    9605
NP_004936                                                              15    9605
NP_001005361                                                           15    9605
NP_001005360                                                           15    9605
NP_001005362                                                           15    9605
NP_001107563                                                           15    9605
NP_060141                                                              15    9605
NP_001247                                                              15    9605
XP_006722284                                                           15    9605
XP_006722283                                                           15    9605
XP_006722282                                                           15    9605
XP_005257952                                                           15    9605
XP_006722281                                                           15    9605
XP_006722280                                                           15    9605
XP_005257949                                                           15    9605
XP_005265822                                                           15    9605
NP_000803                                                              15    9605
>NP_001120972
          Length = 1408

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KESLNIVSF 9
           KE LNIVSF
Sbjct: 759 KEPLNIVSF 767
>XP_006716051
          Length = 1427

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KESLNIVSF 9
           KE LNIVSF
Sbjct: 778 KEPLNIVSF 786
>NP_932765
          Length = 482

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KESLNIVSF 9
           +ESLNIV +
Sbjct: 214 EESLNIVKY 222
>NP_056437
          Length = 482

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KESLNIVSF 9
           +ESLNIV +
Sbjct: 214 EESLNIVKY 222
>NP_783161
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNIV
Sbjct: 436 KETLNIV 442
>NP_056029
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           K+SLNIV
Sbjct: 375 KDSLNIV 381
>NP_001157704
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           K+SLNIV
Sbjct: 375 KDSLNIV 381
>XP_005273513
          Length = 681

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           K+SLNIV
Sbjct: 345 KDSLNIV 351
>XP_005273511
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           K+SLNIV
Sbjct: 375 KDSLNIV 381
>XP_005273512
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           K+SLNIV
Sbjct: 375 KDSLNIV 381
>XP_006715921
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNIV
Sbjct: 436 KETLNIV 442
>XP_005250007
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNIV
Sbjct: 436 KETLNIV 442
>NP_001177645
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNI+
Sbjct: 730 KEALNII 736
>NP_001254651
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  KESLNIVS 8
          KE+LN+++
Sbjct: 62 KEALNVIN 69
>NP_008878
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  KESLNIVS 8
          KE+LN+++
Sbjct: 62 KEALNVIN 69
>NP_004936
          Length = 866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNI+
Sbjct: 726 KEALNII 732
>NP_001005361
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNI+
Sbjct: 730 KEALNII 736
>NP_001005360
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNI+
Sbjct: 730 KEALNII 736
>NP_001005362
          Length = 866

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   KESLNIV 7
           KE+LNI+
Sbjct: 726 KEALNII 732
>NP_001107563
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 488 KEAINILS 495
>NP_060141
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ESLNIVSF 9
           E LNI+SF
Sbjct: 485 EVLNILSF 492
>NP_001247
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 482 KEAINILS 489
>XP_006722284
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 347 KEAINILS 354
>XP_006722283
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 400 KEAINILS 407
>XP_006722282
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 421 KEAINILS 428
>XP_005257952
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 421 KEAINILS 428
>XP_006722281
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 482 KEAINILS 489
>XP_006722280
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 482 KEAINILS 489
>XP_005257949
          Length = 823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE++NI+S
Sbjct: 481 KEAINILS 488
>XP_005265822
          Length = 663

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   KESLNIVS 8
           KE+LN+++
Sbjct: 177 KEALNVIN 184
>NP_000803
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  KESLNIVSF 9
          +ESL ++SF
Sbjct: 7  RESLGLLSF 15
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.130    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 364,562
Number of extensions: 518
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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