Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1482
XP_005252777                                                           17    4312
NP_002102                                                              16    7355
XP_005248127                                                           16    7355
XP_005248125                                                           16    7355
XP_005248124                                                           16    7355
XP_005248123                                                           16    7355
XP_005248122                                                           16    7355
XP_005248121                                                           16    7355
XP_005248120                                                           16    7355
XP_006714059                                                           16    7355
XP_005248119                                                           16    7355
XP_005248118                                                           16    7355
XP_005248117                                                           16    7355
XP_005248116                                                           16    7355
XP_005248115                                                           16    7355
XP_005248114                                                           16    7355
NP_002284                                                              15    9605
NP_001229658                                                           15    9605
NP_001319                                                              15    9605
NP_001012632                                                           15    9605
NP_001275559                                                           15    9605
NP_001275558                                                           15    9605
NP_054828                                                              15    9605
NP_004431                                                              15    9605
XP_005248728                                                           15    9605
XP_005248726                                                           15    9605
XP_005263158                                                           15    9605
XP_005263155                                                           15    9605
XP_005263154                                                           15    9605
XP_005263152                                                           15    9605
XP_005263151                                                           15    9605
XP_006714314                                                           15    9605
XP_005263149                                                           15    9605
XP_005263150                                                           15    9605
XP_006714312                                                           15    9605
XP_005263147                                                           15    9605
XP_005263146                                                           15    9605
XP_005263145                                                           15    9605
XP_005263144                                                           15    9605
XP_005263143                                                           15    9605
XP_006714307                                                           15    9605
XP_005272320                                                           15    9605
XP_005272319                                                           15    9605
XP_005272318                                                           15    9605
>NP_004295
          Length = 1620

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   EAAREILL 9
           EAAREILL
Sbjct: 370 EAAREILL 377
>XP_005252777
          Length = 283

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KEAAREIL 8
           +EAARE+L
Sbjct: 211 EEAAREVL 218
>NP_002102
          Length = 3144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    EAAREILL 9
            EAARE+ L
Sbjct: 2168 EAAREVTL 2175
>XP_005248127
          Length = 1322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248125
          Length = 1439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248124
          Length = 1467

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 250 KEAIRDIVL 258
>XP_005248123
          Length = 1471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 250 KEAIRDIVL 258
>XP_005248122
          Length = 1471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 250 KEAIRDIVL 258
>XP_005248121
          Length = 1543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 322 KEAIRDIVL 330
>XP_005248120
          Length = 1549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_006714059
          Length = 1600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248119
          Length = 1601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248118
          Length = 1602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248117
          Length = 1626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248116
          Length = 1627

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248115
          Length = 1629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>XP_005248114
          Length = 1630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KEAAREILL 9
           KEA R+I+L
Sbjct: 409 KEAIRDIVL 417
>NP_002284
          Length = 1609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KEAAREIL 8
            KEA RE++
Sbjct: 1076 KEAEREVM 1083
>NP_001229658
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EAAREILL 9
           +AAR+ILL
Sbjct: 482 QAARKILL 489
>NP_001319
          Length = 440

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KEAAREI 7
           +EAAREI
Sbjct: 329 EEAAREI 335
>NP_001012632
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KEAAREI 7
           +EAAREI
Sbjct: 318 EEAAREI 324
>NP_001275559
          Length = 279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EAAREILL 9
          +AA+E+LL
Sbjct: 28 QAAKEVLL 35
>NP_001275558
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EAAREILL 9
          +AA+E+LL
Sbjct: 28 QAAKEVLL 35
>NP_054828
          Length = 1390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KEAAREIL 8
           KEA +EIL
Sbjct: 601 KEARKEIL 608
>NP_004431
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EAAREILL 9
          +AA+E+LL
Sbjct: 28 QAAKEVLL 35
>XP_005248728
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EAAREILL 9
          +AA+E+LL
Sbjct: 28 QAAKEVLL 35
>XP_005248726
          Length = 994

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EAAREILL 9
          +AA+E+LL
Sbjct: 28 QAAKEVLL 35
>XP_005263158
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263155
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263154
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263152
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263151
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_006714314
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263149
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263150
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_006714312
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263147
          Length = 1044

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263146
          Length = 1055

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263145
          Length = 1076

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263144
          Length = 1090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_005263143
          Length = 1103

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KEAARE 6
          KEAARE
Sbjct: 45 KEAARE 50
>XP_006714307
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EAAREILL 9
           +AAR+ILL
Sbjct: 264 QAARKILL 271
>XP_005272320
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KEAAREI 7
           +EAAREI
Sbjct: 318 EEAAREI 324
>XP_005272319
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KEAAREI 7
           +EAAREI
Sbjct: 318 EEAAREI 324
>XP_005272318
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KEAAREI 7
           +EAAREI
Sbjct: 329 EEAAREI 335
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.133    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 362,225
Number of extensions: 659
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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