Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              20     299
NP_009297                                                              20     299
XP_006718462                                                           17    2528
XP_006710008                                                           17    2528
NP_957518                                                              17    3301
NP_057439                                                              17    3301
NP_733765                                                              16    5631
NP_001672                                                              16    5631
NP_001273004                                                           16    5631
NP_004311                                                              16    5631
NP_775293                                                              16    5631
NP_001258523                                                           16    5631
NP_653266                                                              16    5631
NP_777613                                                              16    5631
NP_777617                                                              16    5631
NP_777614                                                              16    5631
NP_005164                                                              16    5631
NP_777618                                                              16    5631
NP_777615                                                              16    5631
XP_005256713                                                           16    5631
XP_005253945                                                           16    5631
NP_001001960                                                           16    5631
NP_777616                                                              16    5631
NP_002105                                                              16    7355
NP_001278327                                                           16    7355
NP_001005751                                                           16    7355
NP_008853                                                              16    7355
NP_001075145                                                           16    7355
NP_001075146                                                           16    7355
NP_002449                                                              16    7355
NP_055885                                                              16    7355
NP_001162578                                                           16    7355
NP_919301                                                              16    7355
XP_005266369                                                           16    7355
XP_005266368                                                           16    7355
XP_005266367                                                           16    7355
XP_005266366                                                           16    7355
XP_005266365                                                           16    7355
XP_005266364                                                           16    7355
XP_005266363                                                           16    7355
XP_005266362                                                           16    7355
XP_005266361                                                           16    7355
XP_005266359                                                           16    7355
XP_005266360                                                           16    7355
XP_005266358                                                           16    7355
XP_006717895                                                           16    7355
XP_005269865                                                           16    7355
XP_006717894                                                           16    7355
XP_005269864                                                           16    7355
XP_005269863                                                           16    7355
XP_005249099                                                           16    7355
XP_006712742                                                           16    7355
NP_001162577                                                           16    7355
NP_056077                                                              16    7355
NP_008851                                                              15    9605
NP_001189364                                                           15    9605
NP_001159436                                                           15    9605
NP_001159435                                                           15    9605
NP_001019801                                                           15    9605
NP_001015051                                                           15    9605
NP_001265407                                                           15    9605
NP_997724                                                              15    9605
NP_689733                                                              15    9605
NP_056002                                                              15    9605
NP_115740                                                              15    9605
NP_060557                                                              15    9605
NP_775871                                                              15    9605
XP_006715297                                                           15    9605
XP_006715296                                                           15    9605
XP_006715294                                                           15    9605
XP_006714020                                                           15    9605
XP_005248200                                                           15    9605
XP_006714019                                                           15    9605
XP_006710825                                                           15    9605
NP_001162023                                                           15    9605
>NP_005148
          Length = 1130

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KDKKTSLFS 9
           KDKKT+LFS
Sbjct: 593 KDKKTNLFS 601
>NP_009297
          Length = 1149

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KDKKTSLFS 9
           KDKKT+LFS
Sbjct: 612 KDKKTNLFS 620
>XP_006718462
          Length = 4966

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    DKKTSLF 8
            DKKTS+F
Sbjct: 1015 DKKTSIF 1021
>XP_006710008
          Length = 2014

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    DKKTSLF 8
            DKKTS+F
Sbjct: 1015 DKKTSIF 1021
>NP_957518
          Length = 406

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           +D+KT LFS
Sbjct: 197 RDRKTFLFS 205
>NP_057439
          Length = 455

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           +D+KT LFS
Sbjct: 197 RDRKTFLFS 205
>NP_733765
          Length = 1042

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_001672
          Length = 997

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_001273004
          Length = 869

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KDKKTSLFS 9
          +DKK  LFS
Sbjct: 77 QDKKNMLFS 85
>NP_004311
          Length = 994

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_775293
          Length = 1001

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_001258523
          Length = 353

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KDKKTSLF 8
           K+KK SLF
Sbjct: 153 KEKKESLF 160
>NP_653266
          Length = 492

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KDKKTSLF 8
           K+KK SLF
Sbjct: 292 KEKKESLF 299
>NP_777613
          Length = 1052

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_777617
          Length = 998

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_777614
          Length = 1044

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_005164
          Length = 999

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_777618
          Length = 1029

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_777615
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>XP_005256713
          Length = 1100

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>XP_005253945
          Length = 999

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_001001960
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KDKKTSLF 8
           +DK TSLF
Sbjct: 270 QDKMTSLF 277
>NP_777616
          Length = 1029

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DKK  LFS
Sbjct: 202 QDKKNMLFS 210
>NP_002105
          Length = 2718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KDKKTSLFS 9
            KD+KTS ++
Sbjct: 1958 KDQKTSAYT 1966
>NP_001278327
          Length = 1320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 660 KKTSLF 665
>NP_001005751
          Length = 1341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 660 KKTSLF 665
>NP_008853
          Length = 2000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ KTS+FS
Sbjct: 571 RNSKTSIFS 579
>NP_001075145
          Length = 1951

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ KTS+FS
Sbjct: 571 RNSKTSIFS 579
>NP_001075146
          Length = 1951

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ KTS+FS
Sbjct: 571 RNSKTSIFS 579
>NP_002449
          Length = 5762

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DKKTSLF 8
            D+KTS+F
Sbjct: 1005 DRKTSVF 1011
>NP_055885
          Length = 1564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>NP_001162578
          Length = 1245

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 636 KKTSLF 641
>NP_919301
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKTSLFS 9
           KK+SLFS
Sbjct: 223 KKSSLFS 229
>XP_005266369
          Length = 1564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266368
          Length = 1668

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266367
          Length = 1669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266366
          Length = 1669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266365
          Length = 1669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266364
          Length = 1696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266363
          Length = 1697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266362
          Length = 1697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266361
          Length = 1697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266359
          Length = 1697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266360
          Length = 1697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_005266358
          Length = 1697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           KD+KTS  S
Sbjct: 233 KDRKTSAVS 241
>XP_006717895
          Length = 1297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 688 KKTSLF 693
>XP_005269865
          Length = 1331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 712 KKTSLF 717
>XP_006717894
          Length = 1342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 712 KKTSLF 717
>XP_005269864
          Length = 1364

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 683 KKTSLF 688
>XP_005269863
          Length = 1369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 688 KKTSLF 693
>XP_005249099
          Length = 2026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KDKKTSLFS 9
            KD+KTS ++
Sbjct: 1958 KDQKTSAYT 1966
>XP_006712742
          Length = 2000

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ KTS+FS
Sbjct: 571 RNSKTSIFS 579
>NP_001162577
          Length = 1279

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 660 KKTSLF 665
>NP_056077
          Length = 1320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKTSLF 8
           KKTSLF
Sbjct: 660 KKTSLF 665
>NP_008851
          Length = 1998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ +TSLFS
Sbjct: 568 RNSRTSLFS 576
>NP_001189364
          Length = 2009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ +TSLFS
Sbjct: 568 RNSRTSLFS 576
>NP_001159436
          Length = 1981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ +TSLFS
Sbjct: 568 RNSRTSLFS 576
>NP_001159435
          Length = 2009

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KDKKTSLFS 9
           ++ +TSLFS
Sbjct: 568 RNSRTSLFS 576
>NP_001019801
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           D K SLFS
Sbjct: 236 DSKPSLFS 243
>NP_001015051
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           D K SLFS
Sbjct: 236 DSKPSLFS 243
>NP_001265407
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           D K SLFS
Sbjct: 222 DSKPSLFS 229
>NP_997724
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KDKKTSLF 8
           KDK  SLF
Sbjct: 94  KDKPLSLF 101
>NP_689733
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KDKKTSLFS 9
          K+KK  LFS
Sbjct: 26 KEKKKILFS 34
>NP_056002
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KDKKTSLF 8
           KDK  SLF
Sbjct: 504 KDKHPSLF 511
>NP_115740
          Length = 702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KDKKTSLFS 9
           +DK T LFS
Sbjct: 359 RDKATWLFS 367
>NP_060557
          Length = 674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KTSLFS 9
           KTSLFS
Sbjct: 430 KTSLFS 435
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           DKK  LFS
Sbjct: 151 DKKKPLFS 158
>XP_006715297
          Length = 278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           D K SLFS
Sbjct: 222 DSKPSLFS 229
>XP_006715296
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           D K SLFS
Sbjct: 222 DSKPSLFS 229
>XP_006715294
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   DKKTSLFS 9
           D K SLFS
Sbjct: 222 DSKPSLFS 229
>XP_006714020
          Length = 1097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KDKKTSLF 8
           KDK  SLF
Sbjct: 469 KDKHPSLF 476
>XP_005248200
          Length = 1103

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KDKKTSLF 8
           KDK  SLF
Sbjct: 504 KDKHPSLF 511
>XP_006714019
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   KDKKTSLF 8
           KDK  SLF
Sbjct: 504 KDKHPSLF 511
>XP_006710825
          Length = 669

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KTSLFS 9
           KTSLFS
Sbjct: 425 KTSLFS 430
>NP_001162023
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KTSLFS 9
           KTSLFS
Sbjct: 390 KTSLFS 395
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.118    0.291 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 497,617
Number of extensions: 1016
Number of successful extensions: 75
Number of sequences better than 10000.0: 75
Number of HSP's gapped: 75
Number of HSP's successfully gapped: 75
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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