Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1935
NP_001087241                                                           18    1935
XP_005265799                                                           18    1935
XP_005265798                                                           18    1935
XP_005265797                                                           18    1935
NP_005202                                                              17    3301
NP_001275634                                                           17    3301
NP_001131145                                                           16    5631
NP_703194                                                              16    5631
NP_060090                                                              16    5631
NP_036350                                                              16    5631
NP_004507                                                              16    5631
NP_055523                                                              16    5631
NP_001007526                                                           16    5631
NP_001277284                                                           16    5631
NP_001273142                                                           16    5631
NP_001273141                                                           16    5631
NP_115632                                                              16    5631
XP_006722805                                                           16    5631
XP_006719769                                                           16    5631
NP_775788                                                              16    7355
NP_001271271                                                           16    7355
NP_001019554                                                           16    7355
NP_001445                                                              16    7355
NP_055718                                                              16    7355
XP_006726533                                                           16    7355
XP_006726532                                                           16    7355
XP_006719739                                                           16    7355
XP_006719738                                                           16    7355
XP_005269272                                                           16    7355
XP_006717373                                                           16    7355
XP_002342903                                                           16    7355
XP_005251179                                                           16    7355
XP_005251178                                                           16    7355
XP_006715920                                                           16    7355
XP_005265662                                                           16    7355
NP_001239                                                              16    7355
NP_001726                                                              15    9605
NP_006019                                                              15    9605
NP_073751                                                              15    9605
NP_808211                                                              15    9605
NP_803545                                                              15    9605
NP_775490                                                              15    9605
NP_003703                                                              15    9605
XP_006726866                                                           15    9605
XP_006723983                                                           15    9605
XP_005258189                                                           15    9605
XP_006722402                                                           15    9605
XP_006710157                                                           15    9605
>NP_000213
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KCMAFLASK 9
           K MAFLASK
Sbjct: 778 KGMAFLASK 786
>NP_001087241
          Length = 972

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KCMAFLASK 9
           K MAFLASK
Sbjct: 774 KGMAFLASK 782
>XP_005265799
          Length = 973

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KCMAFLASK 9
           K MAFLASK
Sbjct: 775 KGMAFLASK 783
>XP_005265798
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KCMAFLASK 9
           K MAFLASK
Sbjct: 778 KGMAFLASK 786
>XP_005265797
          Length = 977

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KCMAFLASK 9
           K MAFLASK
Sbjct: 779 KGMAFLASK 787
>NP_005202
          Length = 972

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MAFLASK 9
           MAFLASK
Sbjct: 766 MAFLASK 772
>NP_001275634
          Length = 972

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   MAFLASK 9
           MAFLASK
Sbjct: 766 MAFLASK 772
>NP_001131145
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+A LAS
Sbjct: 202 KCLAALAS 209
>NP_703194
          Length = 690

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+A LAS
Sbjct: 202 KCLAALAS 209
>NP_060090
          Length = 898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+A LAS
Sbjct: 202 KCLAALAS 209
>NP_036350
          Length = 894

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+A LAS
Sbjct: 202 KCLAALAS 209
>NP_004507
          Length = 702

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+A LAS
Sbjct: 202 KCLAALAS 209
>NP_055523
          Length = 2209

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KCMAFLAS 8
            +C+ FLAS
Sbjct: 1918 RCITFLAS 1925
>NP_001007526
          Length = 1432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    CMAFLASK 9
            CMA LAS+
Sbjct: 1005 CMAVLASQ 1012
>NP_001277284
          Length = 1429

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   CMAFLASK 9
           CMA LAS+
Sbjct: 870 CMAVLASQ 877
>NP_001273142
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+AF+ S
Sbjct: 249 KCLAFVPS 256
>NP_001273141
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+AF+ S
Sbjct: 289 KCLAFVPS 296
>NP_115632
          Length = 514

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+AF+ S
Sbjct: 393 KCLAFVPS 400
>XP_006722805
          Length = 855

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KCMAFLAS 8
           KC+A LAS
Sbjct: 202 KCLAALAS 209
>XP_006719769
          Length = 2209

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    KCMAFLAS 8
            +C+ FLAS
Sbjct: 1918 RCITFLAS 1925
>NP_775788
          Length = 176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KCMAFLASK 9
          KC A LAS+
Sbjct: 57 KCSAALASR 65
>NP_001271271
          Length = 177

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KCMAFLASK 9
          KC A LAS+
Sbjct: 57 KCSAALASR 65
>NP_001019554
          Length = 2385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   CMAFLASK 9
           C++FLA++
Sbjct: 145 CLSFLAAR 152
>NP_001445
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   CMAFLASK 9
           CMA  ASK
Sbjct: 131 CMAMQASK 138
>NP_055718
          Length = 2363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   CMAFLASK 9
           C++FLA++
Sbjct: 145 CLSFLAAR 152
>XP_006726533
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KCMAFLASK 9
           +C+ F ASK
Sbjct: 215 ECLKFCASK 223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KCMAFLASK 9
           +C+ F ASK
Sbjct: 576 ECLKFCASK 584
>XP_006726532
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KCMAFLASK 9
           +C+ F ASK
Sbjct: 307 ECLKFCASK 315
>XP_006719739
          Length = 2306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  CMAFLASK 9
          C++FLA++
Sbjct: 88 CLSFLAAR 95
>XP_006719738
          Length = 2340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   CMAFLASK 9
           C++FLA++
Sbjct: 145 CLSFLAAR 152
>XP_005269272
          Length = 2356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   CMAFLASK 9
           C++FLA++
Sbjct: 145 CLSFLAAR 152
>XP_006717373
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  CMAFLA 7
          C+AFLA
Sbjct: 6  CLAFLA 11
>XP_002342903
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KCMAFLASK 9
           +C+ F ASK
Sbjct: 307 ECLKFCASK 315
>XP_005251179
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KCMAFLASK 9
           +C+ F ASK
Sbjct: 307 ECLKFCASK 315
>XP_005251178
          Length = 675

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KCMAFLASK 9
           +C+ F ASK
Sbjct: 307 ECLKFCASK 315
>XP_006715920
          Length = 116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KCMAFLASK 9
          KC A LAS+
Sbjct: 57 KCSAALASR 65
>XP_005265662
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CMAFLA 7
           C+AFLA
Sbjct: 501 CLAFLA 506
>NP_001239
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   CMAFLA 7
           C+AFLA
Sbjct: 501 CLAFLA 506
>NP_001726
          Length = 1676

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2    CMAFLAS 8
            C AFLA+
Sbjct: 1657 CQAFLAN 1663
>NP_006019
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   CMAFL 6
           CMAFL
Sbjct: 309 CMAFL 313
>NP_073751
          Length = 472

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  CMAFLAS 8
          C AF+AS
Sbjct: 59 CAAFIAS 65
>NP_808211
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CMAFLA 7
           CM FLA
Sbjct: 196 CMVFLA 201
>NP_803545
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CMAFLA 7
           CM FLA
Sbjct: 119 CMVFLA 124
>NP_775490
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CMAFLASK 9
           C+ F ASK
Sbjct: 508 CLTFSASK 515
>NP_003703
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   CMAFLA 7
           CM FLA
Sbjct: 175 CMVFLA 180
>XP_006726866
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CMAFLASK 9
           C+ F ASK
Sbjct: 508 CLTFSASK 515
>XP_006723983
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CMAFLASK 9
           C+ F ASK
Sbjct: 508 CLTFSASK 515
>XP_005258189
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  CMAFLAS 8
          C AF+AS
Sbjct: 59 CAAFIAS 65
>XP_006722402
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  CMAFLAS 8
          C AF+AS
Sbjct: 59 CAAFIAS 65
>XP_006710157
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CMAFLASK 9
           C+ F ASK
Sbjct: 508 CLTFSASK 515
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.128    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 398,780
Number of extensions: 482
Number of successful extensions: 50
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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