Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     509
NP_000170                                                              20     509
NP_001268422                                                           20     509
NP_001268423                                                           20     509
XP_005264328                                                           20     509
NP_056386                                                              16    5631
NP_001035150                                                           16    5631
XP_006715520                                                           16    5631
XP_005247500                                                           16    5631
NP_001093879                                                           16    5631
NP_004780                                                              16    7355
NP_005236                                                              16    7355
NP_001014434                                                           16    7355
NP_001008781                                                           16    7355
XP_006718827                                                           16    7355
XP_006717386                                                           16    7355
XP_006714202                                                           16    7355
XP_005262892                                                           16    7355
XP_005262891                                                           16    7355
XP_005245407                                                           16    7355
XP_006711523                                                           16    7355
XP_005245406                                                           16    7355
XP_006711522                                                           16    7355
NP_064589                                                              16    7355
NP_001870                                                              15    9605
NP_060641                                                              15    9605
NP_001238833                                                           15    9605
NP_624302                                                              15    9605
NP_001027019                                                           15    9605
NP_001238834                                                           15    9605
NP_001272369                                                           15    9605
NP_689969                                                              15    9605
NP_001272368                                                           15    9605
NP_001192210                                                           15    9605
XP_005276585                                                           15    9605
XP_370934                                                              15    9605
XP_006719535                                                           15    9605
XP_005269058                                                           15    9605
XP_005248335                                                           15    9605
XP_006714526                                                           15    9605
XP_006713764                                                           15    9605
XP_006713763                                                           15    9605
XP_001716413                                                           15    9605
NP_006230                                                              15    9605
>NP_001268421
          Length = 1230

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAQKTGLIT 9
           KA+KTGLIT
Sbjct: 790 KARKTGLIT 798
>NP_000170
          Length = 1360

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAQKTGLIT 9
           KA+KTGLIT
Sbjct: 920 KARKTGLIT 928
>NP_001268422
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAQKTGLIT 9
           KA+KTGLIT
Sbjct: 618 KARKTGLIT 626
>NP_001268423
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAQKTGLIT 9
           KA+KTGLIT
Sbjct: 618 KARKTGLIT 626
>XP_005264328
          Length = 1261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAQKTGLIT 9
           KA+KTGLIT
Sbjct: 821 KARKTGLIT 829
>NP_056386
          Length = 1112

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQKTGLIT 9
           K+Q TGL T
Sbjct: 264 KSQNTGLTT 272
>NP_001035150
          Length = 175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KAQKTG 6
          KAQKTG
Sbjct: 69 KAQKTG 74
>XP_006715520
          Length = 1056

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQKTGLIT 9
           K+Q TGL T
Sbjct: 208 KSQNTGLTT 216
>XP_005247500
          Length = 152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  KAQKTG 6
          KAQKTG
Sbjct: 46 KAQKTG 51
>NP_001093879
          Length = 1105

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQKTGLIT 9
           K+Q TGL T
Sbjct: 257 KSQNTGLTT 265
>NP_004780
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 300 AQKTGL 305
>NP_005236
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGLIT 9
            KTGLIT
Sbjct: 1192 KTGLIT 1197
>NP_001014434
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 292 AQKTGL 297
>NP_001008781
          Length = 4557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGLIT 9
            KTGLIT
Sbjct: 1201 KTGLIT 1206
>XP_006718827
          Length = 3479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  KTGLIT 9
          KTGLIT
Sbjct: 91 KTGLIT 96
>XP_006717386
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 300 AQKTGL 305
>XP_006714202
          Length = 4588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGLIT 9
            KTGLIT
Sbjct: 1192 KTGLIT 1197
>XP_005262892
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGLIT 9
            KTGLIT
Sbjct: 1192 KTGLIT 1197
>XP_005262891
          Length = 4600

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KTGLIT 9
            KTGLIT
Sbjct: 1192 KTGLIT 1197
>XP_005245407
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 301 AQKTGL 306
>XP_006711523
          Length = 339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 310 AQKTGL 315
>XP_005245406
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 300 AQKTGL 305
>XP_006711522
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 310 AQKTGL 315
>NP_064589
          Length = 397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQKTGL 7
           AQKTGL
Sbjct: 301 AQKTGL 306
>NP_001870
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QKTGLIT 9
           Q+TG+IT
Sbjct: 192 QRTGVIT 198
>NP_060641
          Length = 664

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTGLIT 9
           KTGL+T
Sbjct: 289 KTGLVT 294
>NP_001238833
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTGLIT 9
           KTGL+T
Sbjct: 295 KTGLVT 300
>NP_624302
          Length = 728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QKTGLIT 9
           Q+TG+IT
Sbjct: 192 QRTGVIT 198
>NP_001027019
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QKTGLIT 9
           Q+TG+IT
Sbjct: 192 QRTGVIT 198
>NP_001238834
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTGLIT 9
           KTGL+T
Sbjct: 246 KTGLVT 251
>NP_001272369
          Length = 1385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KAQKTGLIT 9
            K  +TGL+T
Sbjct: 1307 KCHETGLLT 1315
>NP_689969
          Length = 1708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KAQKTGLIT 9
            K  +TGL+T
Sbjct: 1630 KCHETGLLT 1638
>NP_001272368
          Length = 1732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KAQKTGLIT 9
            K  +TGL+T
Sbjct: 1654 KCHETGLLT 1662
>NP_001192210
          Length = 137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QKTGLIT 9
           QK+G+IT
Sbjct: 123 QKSGIIT 129
>XP_005276585
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KAQKTGLI 8
           KAQ+ GL+
Sbjct: 122 KAQRVGLL 129
>XP_370934
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KAQKTGLI 8
           KAQ+ GL+
Sbjct: 122 KAQRVGLL 129
>XP_006719535
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTGLIT 9
           KTGL+T
Sbjct: 295 KTGLVT 300
>XP_005269058
          Length = 651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KTGLIT 9
           KTGL+T
Sbjct: 276 KTGLVT 281
>XP_005248335
          Length = 1423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KAQKTGLIT 9
            K  +TGL+T
Sbjct: 1345 KCHETGLLT 1353
>XP_006714526
          Length = 1670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    KAQKTGLIT 9
            K  +TGL+T
Sbjct: 1592 KCHETGLLT 1600
>XP_006713764
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QKTGLIT 9
           Q+TG+IT
Sbjct: 166 QRTGVIT 172
>XP_006713763
          Length = 702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QKTGLIT 9
           Q+TG+IT
Sbjct: 166 QRTGVIT 172
>XP_001716413
          Length = 764

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KAQKTGLI 8
           KAQ+ GL+
Sbjct: 122 KAQRVGLL 129
>NP_006230
          Length = 753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQKTGLIT 9
           A K GLIT
Sbjct: 582 ADKVGLIT 589
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.123    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 443,043
Number of extensions: 675
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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