Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              19     869
XP_006717990                                                           19     869
XP_006717989                                                           19     869
NP_110413                                                              17    4312
NP_001135973                                                           17    4312
NP_115731                                                              17    4312
NP_001155180                                                           17    4312
XP_006713408                                                           17    4312
NP_001182156                                                           16    5631
NP_060106                                                              16    5631
NP_001128248                                                           16    5631
NP_001128247                                                           16    5631
NP_001171434                                                           16    5631
NP_001128246                                                           16    5631
NP_002422                                                              16    5631
XP_005259075                                                           16    5631
XP_005259074                                                           16    5631
XP_006723312                                                           16    5631
XP_005267745                                                           16    5631
XP_005267744                                                           16    5631
XP_006718728                                                           16    5631
XP_005274241                                                           16    5631
XP_005274240                                                           16    5631
XP_005274239                                                           16    5631
XP_005264064                                                           16    5631
XP_005264063                                                           16    5631
XP_005264062                                                           16    5631
XP_005264061                                                           16    5631
XP_005264059                                                           16    5631
NP_073733                                                              16    5631
NP_982279                                                              16    5631
NP_000072                                                              16    7355
NP_001258779                                                           16    7355
NP_001275949                                                           16    7355
NP_612146                                                              16    7355
NP_659464                                                              16    7355
NP_954863                                                              16    7355
XP_005248961                                                           16    7355
XP_005273087                                                           16    7355
XP_006711794                                                           16    7355
XP_005273086                                                           16    7355
XP_005273085                                                           16    7355
XP_005273084                                                           16    7355
NP_570141                                                              16    7355
NP_001135440                                                           16    7355
NP_803490                                                              15    9605
NP_803491                                                              15    9605
NP_001240830                                                           15    9605
NP_002393                                                              15    9605
NP_001240831                                                           15    9605
NP_001240829                                                           15    9605
NP_001161147                                                           15    9605
NP_872302                                                              15    9605
NP_001161146                                                           15    9605
NP_694854                                                              15    9605
NP_000921                                                              15    9605
NP_127509                                                              15    9605
NP_067012                                                              15    9605
NP_001120645                                                           15    9605
NP_001157849                                                           15    9605
XP_006719292                                                           15    9605
XP_005253377                                                           15    9605
XP_005253376                                                           15    9605
XP_006713145                                                           15    9605
XP_006713144                                                           15    9605
NP_001001660                                                           15    9605
>NP_000305
          Length = 403

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           KAQEAL FY
Sbjct: 147 KAQEALDFY 155
>XP_006717990
          Length = 175

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           KAQEAL FY
Sbjct: 132 KAQEALDFY 140
>XP_006717989
          Length = 388

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           KAQEAL FY
Sbjct: 132 KAQEALDFY 140
>NP_110413
          Length = 482

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  KAQEALY 7
          KA+EALY
Sbjct: 78 KAEEALY 84
>NP_001135973
          Length = 490

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 168 AQELLYIY 175
>NP_115731
          Length = 652

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 330 AQELLYIY 337
>NP_001155180
          Length = 476

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  KAQEALY 7
          KA+EALY
Sbjct: 78 KAEEALY 84
>XP_006713408
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 233 AQELLYIY 240
>NP_001182156
          Length = 1069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           +AQ A+YF+
Sbjct: 571 RAQMAMYFW 579
>NP_060106
          Length = 1214

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           +AQ A+YF+
Sbjct: 571 RAQMAMYFW 579
>NP_001128248
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>NP_001128247
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>NP_001171434
          Length = 267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           K +EALY Y
Sbjct: 198 KLEEALYEY 206
>NP_001128246
          Length = 545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 262 KAEEVEYYY 270
>NP_002422
          Length = 309

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           K +EALY Y
Sbjct: 240 KLEEALYEY 248
>XP_005259075
          Length = 678

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  KAQEALYFY 9
          +AQ A+YF+
Sbjct: 35 RAQMAMYFW 43
>XP_005259074
          Length = 785

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           +AQ A+YF+
Sbjct: 142 RAQMAMYFW 150
>XP_006723312
          Length = 1129

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   KAQEALYFY 9
           +AQ A+YF+
Sbjct: 486 RAQMAMYFW 494
>XP_005267745
          Length = 278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           K +EALY Y
Sbjct: 240 KLEEALYEY 248
>XP_005267744
          Length = 309

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           K +EALY Y
Sbjct: 240 KLEEALYEY 248
>XP_006718728
          Length = 599

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>XP_005274241
          Length = 600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>XP_005274240
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>XP_005274239
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>XP_005264064
          Length = 2282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KAQEALYF 8
            K QEA YF
Sbjct: 1809 KVQEAQYF 1816
>XP_005264063
          Length = 3136

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KAQEALYF 8
            K QEA YF
Sbjct: 2663 KVQEAQYF 2670
>XP_005264062
          Length = 3232

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KAQEALYF 8
            K QEA YF
Sbjct: 2759 KVQEAQYF 2766
>XP_005264061
          Length = 3232

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KAQEALYF 8
            K QEA YF
Sbjct: 2759 KVQEAQYF 2766
>XP_005264059
          Length = 3258

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    KAQEALYF 8
            K QEA YF
Sbjct: 2785 KVQEAQYF 2792
>NP_073733
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KA+E  Y+Y
Sbjct: 339 KAEEVEYYY 347
>NP_982279
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           KAQEAL  Y
Sbjct: 253 KAQEALESY 261
>NP_000072
          Length = 3801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    AQEALYFY 9
            +QEA Y Y
Sbjct: 1680 SQEAFYLY 1687
>NP_001258779
          Length = 193

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  AQEALYFY 9
          A+E+LY++
Sbjct: 15 AEESLYYF 22
>NP_001275949
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALY 7
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_612146
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  QEALYFY 9
          QEALY Y
Sbjct: 59 QEALYKY 65
>NP_659464
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALY 7
           KAQEAL+
Sbjct: 488 KAQEALH 494
>NP_954863
          Length = 522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AQEALYFY 9
           A+E+LY++
Sbjct: 344 AEESLYYF 351
>XP_005248961
          Length = 842

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALY 7
           KAQEAL+
Sbjct: 488 KAQEALH 494
>XP_005273087
          Length = 2788

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    AQEALYFY 9
            +QEA Y Y
Sbjct: 1680 SQEAFYLY 1687
>XP_006711794
          Length = 3022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           +QEA Y Y
Sbjct: 901 SQEAFYLY 908
>XP_005273086
          Length = 3264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    AQEALYFY 9
            +QEA Y Y
Sbjct: 1680 SQEAFYLY 1687
>XP_005273085
          Length = 3755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    AQEALYFY 9
            +QEA Y Y
Sbjct: 1680 SQEAFYLY 1687
>XP_005273084
          Length = 3801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2    AQEALYFY 9
            +QEA Y Y
Sbjct: 1680 SQEAFYLY 1687
>NP_570141
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AQEALYFY 9
           A+E+LY++
Sbjct: 267 AEESLYYF 274
>NP_001135440
          Length = 411

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AQEALYFY 9
           A+E+LY++
Sbjct: 233 AEESLYYF 240
>NP_803490
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 144 AQELLYRY 151
>NP_803491
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 144 AQELLYRY 151
>NP_001240830
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 144 AQELLYRY 151
>NP_002393
          Length = 335

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 153 AQELLYRY 160
>NP_001240831
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 144 AQELLYRY 151
>NP_001240829
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALYFY 9
           AQE LY Y
Sbjct: 139 AQELLYRY 146
>NP_001161147
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 69 AQQTLYF 75
>NP_872302
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           K+QE  YF+
Sbjct: 554 KSQEEEYFF 562
>NP_001161146
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 60 AQQTLYF 66
>NP_694854
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 62 AQQTLYF 68
>NP_000921
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QEALYF 8
           Q+ALYF
Sbjct: 98  QQALYF 103
>NP_127509
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  QEALYF 8
          Q+ALYF
Sbjct: 52 QQALYF 57
>NP_067012
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AQEALYF 8
           AQ+ LYF
Sbjct: 100 AQQTLYF 106
>NP_001120645
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 66 AQQTLYF 72
>NP_001157849
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 76 AQDTLYF 82
>XP_006719292
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   KAQEALYFY 9
           K+QE  YF+
Sbjct: 390 KSQEEEYFF 398
>XP_005253377
          Length = 90

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  KAQEALYF 8
          K  EALYF
Sbjct: 64 KELEALYF 71
>XP_005253376
          Length = 90

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  KAQEALYF 8
          K  EALYF
Sbjct: 64 KELEALYF 71
>XP_006713145
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 60 AQQTLYF 66
>XP_006713144
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AQEALYF 8
          AQ+ LYF
Sbjct: 60 AQQTLYF 66
>NP_001001660
          Length = 90

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  KAQEALYF 8
          K  EALYF
Sbjct: 64 KELEALYF 71
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.136    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 494,675
Number of extensions: 723
Number of successful extensions: 66
Number of sequences better than 10000.0: 66
Number of HSP's gapped: 66
Number of HSP's successfully gapped: 66
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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