Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              20     509
XP_006717990                                                           20     509
XP_006717989                                                           20     509
NP_001126                                                              18    1482
NP_037359                                                              18    1482
XP_006720482                                                           18    1482
NP_001157945                                                           17    2528
NP_060207                                                              17    2528
NP_001020119                                                           17    2528
XP_006710770                                                           17    2528
NP_002891                                                              17    4312
NP_689777                                                              17    4312
NP_060360                                                              17    4312
XP_005250858                                                           17    4312
XP_005250857                                                           17    4312
NP_000503                                                              16    5631
NP_444511                                                              16    5631
NP_597732                                                              16    5631
NP_001132986                                                           16    5631
NP_001132987                                                           16    5631
XP_005262125                                                           16    5631
XP_006722741                                                           16    5631
XP_006722740                                                           16    5631
XP_006722739                                                           16    5631
XP_006722738                                                           16    5631
XP_006722737                                                           16    5631
XP_005259567                                                           16    5631
XP_005256360                                                           16    5631
XP_005256359                                                           16    5631
XP_005256358                                                           16    5631
XP_005253685                                                           16    5631
XP_005253684                                                           16    5631
NP_542938                                                              16    5631
NP_004383                                                              16    5631
NP_542937                                                              16    5631
NP_003950                                                              16    5631
NP_003741                                                              16    7355
NP_055845                                                              16    7355
NP_001025051                                                           16    7355
NP_001018049                                                           16    7355
NP_001159764                                                           16    7355
NP_005634                                                              16    7355
NP_075463                                                              16    7355
NP_003729                                                              16    7355
NP_055646                                                              16    7355
XP_006720708                                                           16    7355
XP_006720707                                                           16    7355
XP_006719812                                                           16    7355
XP_005252608                                                           16    7355
XP_006717553                                                           16    7355
XP_005248148                                                           16    7355
XP_005248147                                                           16    7355
XP_005248146                                                           16    7355
XP_005248145                                                           16    7355
XP_005248144                                                           16    7355
XP_005248143                                                           16    7355
XP_005248142                                                           16    7355
XP_005248141                                                           16    7355
XP_005248140                                                           16    7355
XP_005248139                                                           16    7355
XP_006713495                                                           16    7355
NP_982279                                                              16    7355
NP_037438                                                              15    9605
NP_001092909                                                           15    9605
NP_680086                                                              15    9605
NP_001092286                                                           15    9605
NP_775899                                                              15    9605
NP_004410                                                              15    9605
XP_006718561                                                           15    9605
XP_005251938                                                           15    9605
XP_006710647                                                           15    9605
NP_872401                                                              15    9605
NP_079374                                                              15    9605
>NP_000305
          Length = 403

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KAQEALDF 8
           KAQEALDF
Sbjct: 147 KAQEALDF 154
>XP_006717990
          Length = 175

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KAQEALDF 8
           KAQEALDF
Sbjct: 132 KAQEALDF 139
>XP_006717989
          Length = 388

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   KAQEALDF 8
           KAQEALDF
Sbjct: 132 KAQEALDF 139
>NP_001126
          Length = 2431

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QEALDFC 9
           QEAL+FC
Sbjct: 592 QEALEFC 598
>NP_037359
          Length = 2530

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QEALDFC 9
           QEAL+FC
Sbjct: 592 QEALEFC 598
>XP_006720482
          Length = 2568

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   QEALDFC 9
           QEAL+FC
Sbjct: 592 QEALEFC 598
>NP_001157945
          Length = 605

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALDFC 9
           KAQ+ LD C
Sbjct: 121 KAQQYLDMC 129
>NP_060207
          Length = 547

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALDFC 9
           KAQ+ LD C
Sbjct: 121 KAQQYLDMC 129
>NP_001020119
          Length = 551

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALDFC 9
           KAQ+ LD C
Sbjct: 121 KAQQYLDMC 129
>XP_006710770
          Length = 533

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAQEALDFC 9
           KAQ+ LD C
Sbjct: 103 KAQQYLDMC 111
>NP_002891
          Length = 1247

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           KAQE L+F
Sbjct: 625 KAQEVLEF 632
>NP_689777
          Length = 3997

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EALDFC 9
            EALD+C
Sbjct: 1977 EALDYC 1982
>NP_060360
          Length = 4022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EALDFC 9
            EALD+C
Sbjct: 2002 EALDYC 2007
>XP_005250858
          Length = 4022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EALDFC 9
            EALD+C
Sbjct: 2002 EALDYC 2007
>XP_005250857
          Length = 4022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EALDFC 9
            EALD+C
Sbjct: 2002 EALDYC 2007
>NP_000503
          Length = 522

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   QEALDF 8
           QEALDF
Sbjct: 211 QEALDF 216
>NP_444511
          Length = 599

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 528 KLQEALEF 535
>NP_597732
          Length = 1146

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1043 RAQEALD 1049
>NP_001132986
          Length = 571

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 515 KLQEALEF 522
>NP_001132987
          Length = 432

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 361 KLQEALEF 368
>XP_005262125
          Length = 465

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 394 KLQEALEF 401
>XP_006722741
          Length = 1117

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1014 RAQEALD 1020
>XP_006722740
          Length = 1153

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1050 RAQEALD 1056
>XP_006722739
          Length = 1181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1078 RAQEALD 1084
>XP_006722738
          Length = 1181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1078 RAQEALD 1084
>XP_006722737
          Length = 1182

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1079 RAQEALD 1085
>XP_005259567
          Length = 1217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    KAQEALD 7
            +AQEALD
Sbjct: 1114 RAQEALD 1120
>XP_005256360
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  QEALDF 8
          QEALDF
Sbjct: 26 QEALDF 31
>XP_005256359
          Length = 528

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   QEALDF 8
           QEALDF
Sbjct: 217 QEALDF 222
>XP_005256358
          Length = 575

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   QEALDF 8
           QEALDF
Sbjct: 211 QEALDF 216
>XP_005253685
          Length = 1061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALD 7
           +AQEALD
Sbjct: 653 RAQEALD 659
>XP_005253684
          Length = 1073

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALD 7
           +AQEALD
Sbjct: 665 RAQEALD 671
>NP_542938
          Length = 560

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 493 KLQEALEF 500
>NP_004383
          Length = 506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 439 KLQEALEF 446
>NP_542937
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   KAQEALDF 8
           K QEAL+F
Sbjct: 641 KLQEALEF 648
>NP_003950
          Length = 1068

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALD 7
           +AQEALD
Sbjct: 660 RAQEALD 666
>NP_003741
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AQEALDFC 9
           AQ+A  FC
Sbjct: 178 AQQAFKFC 185
>NP_055845
          Length = 3013

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 415 AQERLDF 421
>NP_001025051
          Length = 894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALDFC 9
           AQEA  FC
Sbjct: 391 AQEAPPFC 398
>NP_001018049
          Length = 894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALDFC 9
           AQEA  FC
Sbjct: 391 AQEAPPFC 398
>NP_001159764
          Length = 1146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 830 AQEPLDF 836
>NP_005634
          Length = 211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QEALDFC 9
           +EALD C
Sbjct: 167 EEALDVC 173
>NP_075463
          Length = 3013

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 463 AQERLDF 469
>NP_003729
          Length = 1203

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 830 AQEPLDF 836
>NP_055646
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    QEALDFC 9
            +EALD C
Sbjct: 1970 REALDIC 1976
>XP_006720708
          Length = 367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  QEALDFC 9
          QE+L FC
Sbjct: 34 QESLPFC 40
>XP_006720707
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  QEALDFC 9
          QE+L FC
Sbjct: 34 QESLPFC 40
>XP_006719812
          Length = 3019

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 460 AQERLDF 466
>XP_005252608
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALDFC 9
           AQEA  FC
Sbjct: 139 AQEAPPFC 146
>XP_006717553
          Length = 725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALDFC 9
           AQEA  FC
Sbjct: 222 AQEAPPFC 229
>XP_005248148
          Length = 3028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 430 AQERLDF 436
>XP_005248147
          Length = 3028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 430 AQERLDF 436
>XP_005248146
          Length = 3073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 522 AQERLDF 528
>XP_005248145
          Length = 3079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 481 AQERLDF 487
>XP_005248144
          Length = 3099

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 507 AQERLDF 513
>XP_005248143
          Length = 3105

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 507 AQERLDF 513
>XP_005248142
          Length = 3114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 522 AQERLDF 528
>XP_005248141
          Length = 3119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 522 AQERLDF 528
>XP_005248140
          Length = 3119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 522 AQERLDF 528
>XP_005248139
          Length = 3120

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQEALDF 8
           AQE LDF
Sbjct: 522 AQERLDF 528
>XP_006713495
          Length = 2969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    QEALDFC 9
            +EALD C
Sbjct: 2014 REALDIC 2020
>NP_982279
          Length = 367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   KAQEALD 7
           KAQEAL+
Sbjct: 253 KAQEALE 259
>NP_037438
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQEALD 7
           AQEALD
Sbjct: 182 AQEALD 187
>NP_001092909
          Length = 1008

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   EALDFC 9
           EALD C
Sbjct: 943 EALDLC 948
>NP_680086
          Length = 256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQEALD 7
           AQEALD
Sbjct: 182 AQEALD 187
>NP_001092286
          Length = 2769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  QEALDFC 9
          QEA +FC
Sbjct: 66 QEAEEFC 72
>NP_775899
          Length = 4462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    AQEALD 7
            AQEALD
Sbjct: 3136 AQEALD 3141
>NP_004410
          Length = 384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QEALDF 8
           QEA+DF
Sbjct: 243 QEAIDF 248
>XP_006718561
          Length = 2794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  QEALDFC 9
          QEA +FC
Sbjct: 91 QEAEEFC 97
>XP_005251938
          Length = 105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  KAQEALDFC 9
          K+Q+  +FC
Sbjct: 82 KSQKIFEFC 90
>XP_006710647
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   EALDFC 9
           EALD C
Sbjct: 438 EALDLC 443
>NP_872401
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   AQEALDFC 9
           AQE LD C
Sbjct: 166 AQELLDPC 173
>NP_079374
          Length = 334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   AQEALD 7
           AQEALD
Sbjct: 188 AQEALD 193
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.133    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 496,211
Number of extensions: 901
Number of successful extensions: 73
Number of sequences better than 10000.0: 73
Number of HSP's gapped: 73
Number of HSP's successfully gapped: 73
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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