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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_644814                                                              17    3301
XP_006721859                                                           17    3301
NP_001231509                                                           17    4312
NP_003487                                                              17    4312
NP_006280                                                              16    5631
XP_006716915                                                           16    5631
XP_005251621                                                           16    5631
NP_054878                                                              16    7355
XP_005265151                                                           16    7355
XP_006713183                                                           16    7355
XP_006713182                                                           16    7355
NP_892018                                                              15    9605
XP_005259608                                                           15    9605

>NP_644814
          Length = 880

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KALEATTK 8
           +A+EATTK
Sbjct: 441 RAIEATTK 448



>XP_006721859
          Length = 844

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   KALEATTK 8
           +A+EATTK
Sbjct: 405 RAIEATTK 412



>NP_001231509
          Length = 3859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    KALEATTKQ 9
            K LEA TKQ
Sbjct: 3446 KILEAKTKQ 3454



>NP_003487
          Length = 3830

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    KALEATTKQ 9
            K LEA TKQ
Sbjct: 3417 KILEAKTKQ 3425



>NP_006280
          Length = 2541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KALEATTK 8
            +ALEATT+
Sbjct: 2144 RALEATTE 2151



>XP_006716915
          Length = 2542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KALEATTK 8
            +ALEATT+
Sbjct: 2144 RALEATTE 2151



>XP_005251621
          Length = 2542

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    KALEATTK 8
            +ALEATT+
Sbjct: 2144 RALEATTE 2151



>NP_054878
          Length = 2564

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KALEATTKQ 9
           ++LEA TKQ
Sbjct: 248 ESLEADTKQ 256



>XP_005265151
          Length = 2058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KALEATTKQ 9
           ++LEA TKQ
Sbjct: 248 ESLEADTKQ 256



>XP_006713183
          Length = 2258

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KALEATTKQ 9
           ++LEA TKQ
Sbjct: 248 ESLEADTKQ 256



>XP_006713182
          Length = 2422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KALEATTKQ 9
           ++LEA TKQ
Sbjct: 248 ESLEADTKQ 256



>NP_892018
          Length = 1059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LEATTK 8
           LEATTK
Sbjct: 432 LEATTK 437



>XP_005259608
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LEATTK 8
           LEATTK
Sbjct: 466 LEATTK 471



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.291    0.101    0.226 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 273,494
Number of extensions: 344
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)