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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_001186978                                                           16    5631
NP_602296                                                              16    7355
NP_001275959                                                           16    7355
NP_116178                                                              16    7355
NP_002035                                                              16    7355
NP_612460                                                              16    7355
NP_001001556                                                           16    7355
NP_001275960                                                           16    7355
XP_006720527                                                           16    7355
XP_005254341                                                           16    7355
XP_006720526                                                           16    7355
XP_006720525                                                           16    7355
XP_005254337                                                           16    7355
XP_006720524                                                           16    7355
XP_005254336                                                           16    7355
XP_005253891                                                           16    7355
XP_006719288                                                           16    7355
XP_005253890                                                           16    7355
XP_005253889                                                           16    7355
XP_005253784                                                           16    7355
XP_005253782                                                           16    7355
XP_006719060                                                           16    7355
XP_006719059                                                           16    7355
XP_006719058                                                           16    7355
XP_005253778                                                           16    7355
XP_005253777                                                           16    7355
XP_005251138                                                           16    7355
XP_006717797                                                           15    9605
NP_056005                                                              15    9605

>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAISLEEIR 9
           +AISLEEIR
Sbjct: 844 EAISLEEIR 852



>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   KAISLEEIR 9
           +AISLEEIR
Sbjct: 844 EAISLEEIR 852



>NP_001186978
          Length = 2715

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    KAISLEEIR 9
            +A+SLE++R
Sbjct: 1285 EAVSLEQLR 1293



>NP_602296
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>NP_001275959
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 282 ISLEELR 288



>NP_116178
          Length = 1025

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           +SLEE+R
Sbjct: 590 VSLEEVR 596



>NP_002035
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 306 ISLEELR 312



>NP_612460
          Length = 347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 258 ISLEELR 264



>NP_001001556
          Length = 447

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 295 ISLEELR 301



>NP_001275960
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 282 ISLEELR 288



>XP_006720527
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 222 ISLEELR 228



>XP_005254341
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 262 ISLEELR 268



>XP_006720526
          Length = 429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 273 ISLEELR 279



>XP_006720525
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 282 ISLEELR 288



>XP_005254337
          Length = 451

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 295 ISLEELR 301



>XP_006720524
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 306 ISLEELR 312



>XP_005254336
          Length = 462

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 306 ISLEELR 312



>XP_005253891
          Length = 268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 179 ISLEELR 185



>XP_006719288
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 336 ISLEELR 342



>XP_005253890
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 360 ISLEELR 366



>XP_005253889
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           ISLEE+R
Sbjct: 360 ISLEELR 366



>XP_005253784
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_005253782
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_006719060
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_006719059
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_006719058
          Length = 384

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_005253778
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_005253777
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           KA+SLE+ R
Sbjct: 135 KALSLEKAR 143



>XP_005251138
          Length = 1037

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ISLEEIR 9
           +SLEE+R
Sbjct: 602 VSLEEVR 608



>XP_006717797
          Length = 238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           K ISLE+I+
Sbjct: 106 KKISLEDIQ 114



>NP_056005
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   KAISLEEIR 9
           K ISLE+I+
Sbjct: 106 KKISLEDIQ 114



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.131    0.294 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 495,289
Number of extensions: 636
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)