Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           20     390
NP_858045                                                              20     390
NP_006525                                                              20     390
NP_001167559                                                           20     390
NP_056164                                                              18    1135
XP_005249030                                                           18    1135
XP_005249029                                                           18    1135
NP_001157789                                                           17    2528
XP_005265039                                                           17    2528
XP_005265038                                                           17    2528
XP_005265035                                                           17    2528
XP_005265034                                                           17    2528
NP_056303                                                              16    5631
NP_072094                                                              16    5631
NP_006725                                                              16    5631
XP_005267014                                                           16    5631
NP_203752                                                              16    7355
NP_001139381                                                           16    7355
NP_065829                                                              16    7355
NP_775764                                                              16    7355
NP_008988                                                              16    7355
NP_001005413                                                           16    7355
NP_127490                                                              16    7355
NP_001008395                                                           16    7355
XP_005269520                                                           16    7355
XP_006712877                                                           16    7355
XP_006712876                                                           16    7355
XP_006712875                                                           16    7355
XP_006712874                                                           16    7355
XP_006712873                                                           16    7355
XP_006712872                                                           16    7355
XP_005246976                                                           16    7355
XP_005246975                                                           16    7355
NP_000089                                                              15    9605
NP_001094383                                                           15    9605
NP_056012                                                              15    9605
NP_001269889                                                           15    9605
NP_001269888                                                           15    9605
NP_060707                                                              15    9605
NP_663619                                                              15    9605
NP_001230056                                                           15    9605
NP_055532                                                              15    9605
NP_055501                                                              15    9605
XP_006723575                                                           15    9605
XP_005259502                                                           15    9605
XP_005259501                                                           15    9605
XP_006720155                                                           15    9605
XP_006720154                                                           15    9605
XP_006720153                                                           15    9605
XP_006720152                                                           15    9605
XP_006720151                                                           15    9605
XP_006720150                                                           15    9605
XP_005267520                                                           15    9605
XP_006720149                                                           15    9605
XP_005267519                                                           15    9605
XP_006720148                                                           15    9605
XP_005264418                                                           15    9605
XP_005270541                                                           15    9605
NP_057706                                                              15    9605
>NP_001167558
          Length = 1423

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAGIMEPMN 9
           KAG MEPMN
Sbjct: 926 KAGRMEPMN 934
>NP_858045
          Length = 1424

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAGIMEPMN 9
           KAG MEPMN
Sbjct: 926 KAGRMEPMN 934
>NP_006525
          Length = 1420

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAGIMEPMN 9
           KAG MEPMN
Sbjct: 926 KAGRMEPMN 934
>NP_001167559
          Length = 1415

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   KAGIMEPMN 9
           KAG MEPMN
Sbjct: 921 KAGRMEPMN 929
>NP_056164
          Length = 1079

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAGIMEPMN 9
           K G+ EPMN
Sbjct: 866 KTGVTEPMN 874
>XP_005249030
          Length = 1079

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAGIMEPMN 9
           K G+ EPMN
Sbjct: 866 KTGVTEPMN 874
>XP_005249029
          Length = 1079

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAGIMEPMN 9
           K G+ EPMN
Sbjct: 866 KTGVTEPMN 874
>NP_001157789
          Length = 2633

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KAGIMEPMN 9
            K G++EP+N
Sbjct: 1493 KTGLVEPVN 1501
>XP_005265039
          Length = 2177

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KAGIMEPMN 9
            K G++EP+N
Sbjct: 1493 KTGLVEPVN 1501
>XP_005265038
          Length = 2181

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KAGIMEPMN 9
            K G++EP+N
Sbjct: 1493 KTGLVEPVN 1501
>XP_005265035
          Length = 2609

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KAGIMEPMN 9
            K G++EP+N
Sbjct: 1493 KTGLVEPVN 1501
>XP_005265034
          Length = 2622

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    KAGIMEPMN 9
            K G++EP+N
Sbjct: 1493 KTGLVEPVN 1501
>NP_056303
          Length = 385

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AGIMEPMN 9
           AG++EP N
Sbjct: 301 AGVVEPEN 308
>NP_072094
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AGIMEPMN 9
           AG++EP N
Sbjct: 277 AGVVEPEN 284
>NP_006725
          Length = 2446

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           + GIMEP+
Sbjct: 452 RKGIMEPL 459
>XP_005267014
          Length = 2446

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           + GIMEP+
Sbjct: 452 RKGIMEPL 459
>NP_203752
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 566 KRGVLEPL 573
>NP_001139381
          Length = 1390

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 558 KRGVLEPL 565
>NP_065829
          Length = 1907

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GIMEPMN 9
           GI+EPM+
Sbjct: 785 GIIEPMD 791
>NP_775764
          Length = 773

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   KAGIMEPMN 9
           +AG+M+P++
Sbjct: 334 QAGLMDPVD 342
>NP_008988
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AGIMEPM 8
          AGI+EP+
Sbjct: 20 AGILEPV 26
>NP_001005413
          Length = 230

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AGIMEPM 8
          AGI+EP+
Sbjct: 20 AGILEPV 26
>NP_127490
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AGIMEPM 8
          AGI+EP+
Sbjct: 20 AGILEPV 26
>NP_001008395
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   IMEPMN 9
           I+EPMN
Sbjct: 282 ILEPMN 287
>XP_005269520
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AGIMEPM 8
          AGI+EP+
Sbjct: 20 AGILEPV 26
>XP_006712877
          Length = 1677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 382 KRGVLEPL 389
>XP_006712876
          Length = 1783

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 488 KRGVLEPL 495
>XP_006712875
          Length = 1826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 531 KRGVLEPL 538
>XP_006712874
          Length = 1836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 541 KRGVLEPL 548
>XP_006712873
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 566 KRGVLEPL 573
>XP_006712872
          Length = 1861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 566 KRGVLEPL 573
>XP_005246976
          Length = 1864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 569 KRGVLEPL 576
>XP_005246975
          Length = 1871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   KAGIMEPM 8
           K G++EP+
Sbjct: 576 KRGVLEPL 583
>NP_000089
          Length = 658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   KAGIMEP 7
           +AG++EP
Sbjct: 167 RAGLLEP 173
>NP_001094383
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 695 KVGIVEP 701
>NP_056012
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 680 KVGIVEP 686
>NP_001269889
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   KAGIMEP 7
           KAG+++P
Sbjct: 260 KAGLLQP 266
>NP_001269888
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   KAGIMEP 7
           KAG+++P
Sbjct: 260 KAGLLQP 266
>NP_060707
          Length = 1150

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   KAGIMEP 7
           KAG+++P
Sbjct: 275 KAGLLQP 281
>NP_663619
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  AGIMEPMN 9
          +GI+ P+N
Sbjct: 14 SGILRPLN 21
>NP_001230056
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 605 KVGIVEP 611
>NP_055532
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           KAG+ EP
Sbjct: 443 KAGLSEP 449
>NP_055501
          Length = 1070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           KAG M+P
Sbjct: 683 KAGAMQP 689
>XP_006723575
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           KAG+ EP
Sbjct: 443 KAGLSEP 449
>XP_005259502
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           KAG+ EP
Sbjct: 456 KAGLSEP 462
>XP_005259501
          Length = 1070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           KAG M+P
Sbjct: 683 KAGAMQP 689
>XP_006720155
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 672 KVGIVEP 678
>XP_006720154
          Length = 894

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 685 KVGIVEP 691
>XP_006720153
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 696 KVGIVEP 702
>XP_006720152
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 681 KVGIVEP 687
>XP_006720151
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 685 KVGIVEP 691
>XP_006720150
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 688 KVGIVEP 694
>XP_005267520
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 691 KVGIVEP 697
>XP_006720149
          Length = 922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 692 KVGIVEP 698
>XP_005267519
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 695 KVGIVEP 701
>XP_006720148
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   KAGIMEP 7
           K GI+EP
Sbjct: 696 KVGIVEP 702
>XP_005264418
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  AGIMEPMN 9
          +GI+ P+N
Sbjct: 14 SGILRPLN 21
>XP_005270541
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   KAGIMEP 7
           +AG++EP
Sbjct: 167 RAGLLEP 173
>NP_057706
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  AGIMEPMN 9
          +GI+ P+N
Sbjct: 14 SGILRPLN 21
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.130    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 644,132
Number of extensions: 1165
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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