Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1135
XP_005271621                                                           18    1135
XP_006718907                                                           18    1135
XP_006718906                                                           18    1135
XP_005271619                                                           18    1135
XP_005271618                                                           18    1135
NP_005302                                                              17    2528
NP_001001549                                                           17    2528
NP_001001550                                                           17    2528
NP_001001555                                                           17    2528
XP_005271824                                                           17    2528
XP_006715768                                                           17    2528
XP_005271823                                                           17    2528
XP_005271822                                                           17    2528
XP_006715767                                                           17    2528
XP_006715766                                                           17    2528
XP_005271818                                                           17    2528
XP_005271817                                                           17    2528
NP_057712                                                              17    4312
NP_567823                                                              17    4312
NP_004103                                                              17    4312
XP_005252516                                                           17    4312
XP_005252514                                                           17    4312
XP_005252511                                                           17    4312
NP_001253974                                                           16    5631
NP_005780                                                              16    5631
NP_789839                                                              16    5631
NP_000104                                                              16    7355
NP_001231800                                                           16    7355
NP_001334                                                              16    7355
NP_001026885                                                           16    7355
NP_116238                                                              16    7355
NP_001121363                                                           15    9605
NP_004521                                                              15    9605
NP_078973                                                              15    9605
NP_001008537                                                           15    9605
XP_006724709                                                           15    9605
XP_006724708                                                           15    9605
XP_006717679                                                           15    9605
XP_005269551                                                           15    9605
XP_006714215                                                           15    9605
XP_006714214                                                           15    9605
XP_005262915                                                           15    9605
XP_005265544                                                           15    9605
XP_005265543                                                           15    9605
XP_005265542                                                           15    9605
NP_055806                                                              15    9605
NP_653188                                                              15    9605
>NP_000042
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KAAMNFF 7
           KAAMNFF
Sbjct: 621 KAAMNFF 627
>XP_005271621
          Length = 2708

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KAAMNFF 7
           KAAMNFF
Sbjct: 273 KAAMNFF 279
>XP_006718907
          Length = 3050

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KAAMNFF 7
           KAAMNFF
Sbjct: 621 KAAMNFF 627
>XP_006718906
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KAAMNFF 7
           KAAMNFF
Sbjct: 621 KAAMNFF 627
>XP_005271619
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KAAMNFF 7
           KAAMNFF
Sbjct: 621 KAAMNFF 627
>XP_005271618
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   KAAMNFF 7
           KAAMNFF
Sbjct: 621 KAAMNFF 627
>NP_005302
          Length = 594

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 256 KNPMNFFPE 264
>NP_001001549
          Length = 548

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 256 KNPMNFFPE 264
>NP_001001550
          Length = 536

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 198 KNPMNFFPE 206
>NP_001001555
          Length = 536

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 198 KNPMNFFPE 206
>XP_005271824
          Length = 407

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  KAAMNFFPK 9
          K  MNFFP+
Sbjct: 69 KNPMNFFPE 77
>XP_006715768
          Length = 536

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 198 KNPMNFFPE 206
>XP_005271823
          Length = 536

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 198 KNPMNFFPE 206
>XP_005271822
          Length = 536

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 198 KNPMNFFPE 206
>XP_006715767
          Length = 588

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 250 KNPMNFFPE 258
>XP_006715766
          Length = 594

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 256 KNPMNFFPE 264
>XP_005271818
          Length = 594

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 256 KNPMNFFPE 264
>XP_005271817
          Length = 594

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K  MNFFP+
Sbjct: 256 KNPMNFFPE 264
>NP_057712
          Length = 647

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K A+N FPK
Sbjct: 175 KMAVNSFPK 183
>NP_567823
          Length = 544

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K A+N FPK
Sbjct: 175 KMAVNSFPK 183
>NP_004103
          Length = 225

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           KAA +F PK
Sbjct: 191 KAAAHFLPK 199
>XP_005252516
          Length = 550

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K A+N FPK
Sbjct: 181 KMAVNSFPK 189
>XP_005252514
          Length = 602

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K A+N FPK
Sbjct: 130 KMAVNSFPK 138
>XP_005252511
          Length = 653

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   KAAMNFFPK 9
           K A+N FPK
Sbjct: 181 KMAVNSFPK 189
>NP_001253974
          Length = 265

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  NFFPK 9
          NFFPK
Sbjct: 43 NFFPK 47
>NP_005780
          Length = 254

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  NFFPK 9
          NFFPK
Sbjct: 32 NFFPK 36
>NP_789839
          Length = 267

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5  NFFPK 9
          NFFPK
Sbjct: 32 NFFPK 36
>NP_000104
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   MNFFPK 9
           M FFPK
Sbjct: 304 MTFFPK 309
>NP_001231800
          Length = 749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           A +NFFP
Sbjct: 261 ANLNFFP 267
>NP_001334
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           A +NFFP
Sbjct: 282 ANLNFFP 288
>NP_001026885
          Length = 1027

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AMNFFPK 9
           A+N FPK
Sbjct: 539 AVNIFPK 545
>NP_116238
          Length = 303

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   KAAMNFFP 8
           +A  NFFP
Sbjct: 172 EAVKNFFP 179
>NP_001121363
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  AAMNFFPK 9
          A  NFFP+
Sbjct: 58 ANYNFFPR 65
>NP_004521
          Length = 660

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   AAMNFFPK 9
           A  NFFP+
Sbjct: 108 ANYNFFPR 115
>NP_078973
          Length = 395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AAMNFFP 8
          AA+N FP
Sbjct: 45 AAVNLFP 51
>NP_001008537
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   AAMNFFP 8
           A  NFFP
Sbjct: 333 AQFNFFP 339
>XP_006724709
          Length = 1486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   AAMNFFP 8
           A  NFFP
Sbjct: 333 AQFNFFP 339
>XP_006724708
          Length = 1486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   AAMNFFP 8
           A  NFFP
Sbjct: 333 AQFNFFP 339
>XP_006717679
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   MNFFPK 9
           +NFFP+
Sbjct: 176 LNFFPE 181
>XP_005269551
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   MNFFPK 9
           +NFFP+
Sbjct: 277 LNFFPE 282
>XP_006714215
          Length = 3544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           +AMN FP
Sbjct: 750 SAMNVFP 756
>XP_006714214
          Length = 3544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           +AMN FP
Sbjct: 750 SAMNVFP 756
>XP_005262915
          Length = 3544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           +AMN FP
Sbjct: 750 SAMNVFP 756
>XP_005265544
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  AAMNFFP 8
          AA+N FP
Sbjct: 45 AAVNLFP 51
>XP_005265543
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           AA+N FP
Sbjct: 146 AAVNLFP 152
>XP_005265542
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           AA+N FP
Sbjct: 146 AAVNLFP 152
>NP_055806
          Length = 3526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   AAMNFFP 8
           +AMN FP
Sbjct: 732 SAMNVFP 738
>NP_653188
          Length = 506

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   MNFFPK 9
           +NFFP+
Sbjct: 344 LNFFPE 349
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.135    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 455,748
Number of extensions: 477
Number of successful extensions: 48
Number of sequences better than 10000.0: 48
Number of HSP's gapped: 48
Number of HSP's successfully gapped: 48
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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