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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              19     869
NP_000593                                                              17    2528
NP_683765                                                              16    7355
NP_000375                                                              15    9605
NP_000283                                                              15    9605
NP_003985                                                              15    9605
NP_000890                                                              15    9605
NP_001076                                                              15    9605
XP_006724561                                                           15    9605
XP_005274607                                                           15    9605
XP_005274605                                                           15    9605
XP_006724560                                                           15    9605
XP_006724559                                                           15    9605

>NP_073713
          Length = 985

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   IVSAMLAPE 9
           IVS MLAPE
Sbjct: 550 IVSTMLAPE 558



>NP_000593
          Length = 402

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   IVSAMLAPE 9
           IVSA +APE
Sbjct: 365 IVSARMAPE 373



>NP_683765
          Length = 423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  IVSAMLAP 8
          +VSA LAP
Sbjct: 90 LVSAFLAP 97



>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   IVSAMLAP 8
           I+SA+L P
Sbjct: 160 IISALLVP 167



>NP_000283
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IVSAMLAPE 9
           I+SA L PE
Sbjct: 863 IISAPLPPE 871



>NP_003985
          Length = 245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IVSAMLAPE 9
           +VS+ L+PE
Sbjct: 164 VVSSTLSPE 172



>NP_000890
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IVSAMLAPE 9
           +VS+ L+PE
Sbjct: 164 VVSSTLSPE 172



>NP_001076
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VSAMLAPE 9
           V AML PE
Sbjct: 287 VEAMLLPE 294



>XP_006724561
          Length = 1061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IVSAMLAPE 9
           I+SA L PE
Sbjct: 681 IISAPLPPE 689



>XP_005274607
          Length = 1207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IVSAMLAPE 9
           I+SA L PE
Sbjct: 863 IISAPLPPE 871



>XP_005274605
          Length = 1217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IVSAMLAPE 9
           I+SA L PE
Sbjct: 863 IISAPLPPE 871



>XP_006724560
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IVSAMLAPE 9
           I+SA L PE
Sbjct: 863 IISAPLPPE 871



>XP_006724559
          Length = 1243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   IVSAMLAPE 9
           I+SA L PE
Sbjct: 863 IISAPLPPE 871



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.126    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 445,724
Number of extensions: 534
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)