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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           19     665
NP_004561                                                              19     665
NP_001275703                                                           19     665
XP_005253450                                                           19     665
XP_006719151                                                           19     665
XP_005254910                                                           17    3301
XP_005254909                                                           17    3301
XP_005254908                                                           17    3301
XP_005254907                                                           17    3301
XP_005254906                                                           17    3301
XP_005254900                                                           17    3301
XP_005254899                                                           17    3301
XP_005254898                                                           17    3301
XP_005254897                                                           17    3301
XP_006720441                                                           17    3301
XP_006720440                                                           17    3301
XP_006720439                                                           17    3301
XP_005254896                                                           17    3301
NP_002430                                                              16    5631
NP_001139280                                                           16    7355
NP_000623                                                              16    7355
NP_001025226                                                           16    7355
NP_078948                                                              16    7355
NP_001164                                                              16    7355
XP_006721108                                                           16    7355
XP_005267693                                                           16    7355
XP_005267692                                                           16    7355
XP_005263278                                                           16    7355
XP_005263277                                                           16    7355
NP_005372                                                              15    9605
NP_004882                                                              15    9605

>NP_001275701
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IVKSKTLFV 9
            IVKSKT+FV
Sbjct: 1453 IVKSKTVFV 1461



>NP_004561
          Length = 1445

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IVKSKTLFV 9
            IVKSKT+FV
Sbjct: 1412 IVKSKTVFV 1420



>NP_001275703
          Length = 1264

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IVKSKTLFV 9
            IVKSKT+FV
Sbjct: 1231 IVKSKTVFV 1239



>XP_005253450
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IVKSKTLFV 9
            IVKSKT+FV
Sbjct: 1453 IVKSKTVFV 1461



>XP_006719151
          Length = 1486

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    IVKSKTLFV 9
            IVKSKT+FV
Sbjct: 1453 IVKSKTVFV 1461



>XP_005254910
          Length = 1523

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IVKSKTLF 8
           ++KSKTLF
Sbjct: 381 LLKSKTLF 388



>XP_005254909
          Length = 1536

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IVKSKTLF 8
           ++KSKTLF
Sbjct: 394 LLKSKTLF 401



>XP_005254908
          Length = 1536

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   IVKSKTLF 8
           ++KSKTLF
Sbjct: 394 LLKSKTLF 401



>XP_005254907
          Length = 2590

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1448 LLKSKTLF 1455



>XP_005254906
          Length = 2675

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1533 LLKSKTLF 1540



>XP_005254900
          Length = 2843

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1701 LLKSKTLF 1708



>XP_005254899
          Length = 2861

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1719 LLKSKTLF 1726



>XP_005254898
          Length = 2874

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1741 LLKSKTLF 1748



>XP_005254897
          Length = 2876

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1741 LLKSKTLF 1748



>XP_006720441
          Length = 2881

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1741 LLKSKTLF 1748



>XP_006720440
          Length = 2882

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1741 LLKSKTLF 1748



>XP_006720439
          Length = 2882

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1741 LLKSKTLF 1748



>XP_005254896
          Length = 2883

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    IVKSKTLF 8
            ++KSKTLF
Sbjct: 1741 LLKSKTLF 1748



>NP_002430
          Length = 1137

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    VKSKTLFV 9
            VKS TLFV
Sbjct: 1001 VKSLTLFV 1008



>NP_001139280
          Length = 1153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KSKTLF 8
           KSKTLF
Sbjct: 182 KSKTLF 187



>NP_000623
          Length = 1152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KSKTLF 8
           KSKTLF
Sbjct: 182 KSKTLF 187



>NP_001025226
          Length = 1502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KSKTLF 8
            KSKTLF
Sbjct: 1156 KSKTLF 1161



>NP_078948
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IVKSKTLFV 9
           ++++KT+FV
Sbjct: 547 LIEAKTIFV 555



>NP_001164
          Length = 1501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KSKTLF 8
            KSKTLF
Sbjct: 1156 KSKTLF 1161



>XP_006721108
          Length = 613

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KSKTLF 8
           KSKTLF
Sbjct: 182 KSKTLF 187



>XP_005267693
          Length = 1502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KSKTLF 8
            KSKTLF
Sbjct: 1156 KSKTLF 1161



>XP_005267692
          Length = 1502

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KSKTLF 8
            KSKTLF
Sbjct: 1156 KSKTLF 1161



>XP_005263278
          Length = 620

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IVKSKTLFV 9
           ++++KT+FV
Sbjct: 264 LIEAKTIFV 272



>XP_005263277
          Length = 759

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   IVKSKTLFV 9
           ++++KT+FV
Sbjct: 403 LIEAKTIFV 411



>NP_005372
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SKTLFV 9
           SKTLFV
Sbjct: 571 SKTLFV 576



>NP_004882
          Length = 65

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  IVKSKTLFV 9
          +VK + LFV
Sbjct: 49 VVKQRVLFV 57



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.142    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,352
Number of extensions: 350
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)