Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
XP_005273301                                                           17    3301
NP_003263                                                              17    3301
NP_077306                                                              16    5631
NP_055901                                                              16    5631
NP_057411                                                              16    5631
XP_005276791                                                           16    5631
XP_005261690                                                           16    5631
XP_005244909                                                           16    5631
NP_001934                                                              16    7355
NP_001010860                                                           16    7355
NP_001182180                                                           15    9605
NP_778239                                                              15    9605
NP_001182181                                                           15    9605
NP_000775                                                              15    9605
NP_064550                                                              15    9605
NP_001182183                                                           15    9605
NP_056359                                                              15    9605
NP_001182178                                                           15    9605
NP_001028676                                                           15    9605
NP_001275517                                                           15    9605
NP_778243                                                              15    9605
NP_001182179                                                           15    9605
NP_001002843                                                           15    9605
NP_060131                                                              15    9605
NP_001182177                                                           15    9605
NP_848642                                                              15    9605
NP_001070142                                                           15    9605
NP_000479                                                              15    9605
XP_005257707                                                           15    9605
XP_005254541                                                           15    9605
XP_005254540                                                           15    9605
XP_005254538                                                           15    9605
XP_006716855                                                           15    9605
XP_006716854                                                           15    9605
XP_006716799                                                           15    9605
XP_005251452                                                           15    9605
XP_005251451                                                           15    9605
XP_005251450                                                           15    9605
XP_006716798                                                           15    9605
XP_006716797                                                           15    9605
XP_006716796                                                           15    9605
XP_005245255                                                           15    9605
XP_005270859                                                           15    9605
XP_005270858                                                           15    9605
XP_005270856                                                           15    9605
NP_803190                                                              15    9605
NP_077012                                                              15    9605
NP_001028260                                                           15    9605
>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   IVIKSKYSP 9
           IVIKS+YSP
Sbjct: 362 IVIKSEYSP 370
>XP_005273301
          Length = 323

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KSKYSP 9
           KSKYSP
Sbjct: 144 KSKYSP 149
>NP_003263
          Length = 399

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KSKYSP 9
           KSKYSP
Sbjct: 144 KSKYSP 149
>NP_077306
          Length = 730

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IVIKSKYS 8
           +V+KSK+S
Sbjct: 396 VVLKSKFS 403
>NP_055901
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   IVIKSKYSP 9
           +VI+S+Y P
Sbjct: 460 VVIRSQYVP 468
>NP_057411
          Length = 384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           +KS YSP
Sbjct: 373 VKSNYSP 379
>XP_005276791
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IVIKSKYS 8
           +V+KSK+S
Sbjct: 156 VVLKSKFS 163
>XP_005261690
          Length = 316

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           +KS YSP
Sbjct: 305 VKSNYSP 311
>XP_005244909
          Length = 490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   IVIKSKYS 8
           +V+KSK+S
Sbjct: 156 VVLKSKFS 163
>NP_001934
          Length = 1118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           I+SKY P
Sbjct: 364 IRSKYKP 370
>NP_001010860
          Length = 674

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   IKSKYS 8
           IKSKYS
Sbjct: 428 IKSKYS 433
>NP_001182180
          Length = 292

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 189 VVIKDKY 195
>NP_778239
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 203 VVIKDKY 209
>NP_001182181
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 131 VVIKDKY 137
>NP_000775
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  IVIKSKYSP 9
          ++ K+KY P
Sbjct: 86 VLYKAKYGP 94
>NP_064550
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KSKYSP 9
           KSK+SP
Sbjct: 154 KSKFSP 159
>NP_001182183
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 135 VVIKDKY 141
>NP_056359
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VIKSKYSP 9
           V+KS Y P
Sbjct: 142 VVKSMYPP 149
>NP_001182178
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 189 VVIKDKY 195
>NP_001028676
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VIKSKYSP 9
           V+KS Y P
Sbjct: 142 VVKSMYPP 149
>NP_001275517
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KSKYSP 9
           KSKY P
Sbjct: 584 KSKYKP 589
>NP_778243
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 189 VVIKDKY 195
>NP_001182179
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 149 VVIKDKY 155
>NP_001002843
          Length = 966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KSKYSP 9
           KSKY P
Sbjct: 571 KSKYKP 576
>NP_060131
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KSKYSP 9
           KSKY P
Sbjct: 584 KSKYKP 589
>NP_001182177
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 203 VVIKDKY 209
>NP_848642
          Length = 179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  IKSKYSP 9
          IK+KY P
Sbjct: 32 IKAKYPP 38
>NP_001070142
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           +KSKY P
Sbjct: 199 LKSKYFP 205
>NP_000479
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KSKYSP 9
           KSK+SP
Sbjct: 258 KSKFSP 263
>XP_005257707
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           +KSKY P
Sbjct: 179 LKSKYFP 185
>XP_005254541
          Length = 890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KSKYSP 9
           KSKY P
Sbjct: 748 KSKYKP 753
>XP_005254540
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KSKYSP 9
           KSKY P
Sbjct: 748 KSKYKP 753
>XP_005254538
          Length = 993

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   KSKYSP 9
           KSKY P
Sbjct: 748 KSKYKP 753
>XP_006716855
          Length = 254

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 101 VVIKDKY 107
>XP_006716854
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   IVIKSKY 7
           +VIK KY
Sbjct: 189 VVIKDKY 195
>XP_006716799
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 249 VKSRFSP 255
>XP_005251452
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 249 VKSRFSP 255
>XP_005251451
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 249 VKSRFSP 255
>XP_005251450
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 249 VKSRFSP 255
>XP_006716798
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 278 VKSRFSP 284
>XP_006716797
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 278 VKSRFSP 284
>XP_006716796
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   IKSKYSP 9
           +KS++SP
Sbjct: 290 VKSRFSP 296
>XP_005245255
          Length = 416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KSKYSP 9
           KSK+SP
Sbjct: 210 KSKFSP 215
>XP_005270859
          Length = 141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  IKSKYSP 9
          IK+KY P
Sbjct: 32 IKAKYPP 38
>XP_005270858
          Length = 148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  IKSKYSP 9
          IK+KY P
Sbjct: 32 IKAKYPP 38
>XP_005270856
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  IKSKYSP 9
          IK+KY P
Sbjct: 32 IKAKYPP 38
>NP_803190
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           +KSKY P
Sbjct: 179 LKSKYFP 185
>NP_077012
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   IKSKYSP 9
           +KSKY P
Sbjct: 179 LKSKYFP 185
>NP_001028260
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VIKSKYSP 9
           V+KSK+ P
Sbjct: 121 VLKSKFLP 128
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.132    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 453,275
Number of extensions: 388
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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