Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     665
NP_000240                                                              19     665
XP_005265218                                                           19     665
NP_001243599                                                           18    1935
NP_569710                                                              18    1935
NP_005691                                                              18    1935
NP_079390                                                              17    3301
XP_006719673                                                           17    3301
XP_005269218                                                           17    3301
XP_005269217                                                           17    3301
XP_006719672                                                           17    3301
XP_005269215                                                           17    3301
XP_006722374                                                           16    5631
XP_006722373                                                           16    5631
XP_006722370                                                           16    5631
XP_006722365                                                           16    5631
NP_006337                                                              16    7355
NP_056496                                                              16    7355
NP_001106853                                                           16    7355
NP_065928                                                              16    7355
XP_005256667                                                           16    7355
XP_005256666                                                           16    7355
XP_005256665                                                           16    7355
XP_005256664                                                           16    7355
XP_005256662                                                           16    7355
XP_005256527                                                           16    7355
XP_006719819                                                           16    7355
XP_006712889                                                           16    7355
NP_892006                                                              15    9605
NP_149062                                                              15    9605
XP_006715488                                                           15    9605
XP_006715487                                                           15    9605
XP_006715486                                                           15    9605
XP_006715485                                                           15    9605
XP_006715484                                                           15    9605
XP_006715483                                                           15    9605
XP_006715482                                                           15    9605
XP_006715481                                                           15    9605
XP_006715480                                                           15    9605
XP_006715479                                                           15    9605
XP_006715478                                                           15    9605
XP_006715477                                                           15    9605
XP_006715476                                                           15    9605
XP_006715475                                                           15    9605
XP_006715474                                                           15    9605
XP_006715473                                                           15    9605
XP_006715472                                                           15    9605
XP_006715471                                                           15    9605
XP_006715470                                                           15    9605
>NP_001245200
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  TSKLQSFE 9
          TSKLQSFE
Sbjct: 82 TSKLQSFE 89
>NP_000240
          Length = 756

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  TSKLQSFE 9
          TSKLQSFE
Sbjct: 82 TSKLQSFE 89
>XP_005265218
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2  TSKLQSFE 9
          TSKLQSFE
Sbjct: 82 TSKLQSFE 89
>NP_001243599
          Length = 707

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ITSKLQSFE 9
           +TSKL+S+E
Sbjct: 200 VTSKLKSYE 208
>NP_569710
          Length = 737

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ITSKLQSFE 9
           +TSKL+S+E
Sbjct: 230 VTSKLKSYE 238
>NP_005691
          Length = 737

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ITSKLQSFE 9
           +TSKL+S+E
Sbjct: 230 VTSKLKSYE 238
>NP_079390
          Length = 2479

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    ITSKLQSFE 9
            ITSKLQ  E
Sbjct: 1224 ITSKLQKME 1232
>XP_006719673
          Length = 1997

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ITSKLQSFE 9
           ITSKLQ  E
Sbjct: 742 ITSKLQKME 750
>XP_005269218
          Length = 2009

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ITSKLQSFE 9
           ITSKLQ  E
Sbjct: 754 ITSKLQKME 762
>XP_005269217
          Length = 2353

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    ITSKLQSFE 9
            ITSKLQ  E
Sbjct: 1255 ITSKLQKME 1263
>XP_006719672
          Length = 2510

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    ITSKLQSFE 9
            ITSKLQ  E
Sbjct: 1255 ITSKLQKME 1263
>XP_005269215
          Length = 2510

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    ITSKLQSFE 9
            ITSKLQ  E
Sbjct: 1255 ITSKLQKME 1263
>XP_006722374
          Length = 944

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TSKLQSFE 9
           T K+QSFE
Sbjct: 728 TIKMQSFE 735
>XP_006722373
          Length = 948

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TSKLQSFE 9
           T K+QSFE
Sbjct: 732 TIKMQSFE 739
>XP_006722370
          Length = 956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TSKLQSFE 9
           T K+QSFE
Sbjct: 740 TIKMQSFE 747
>XP_006722365
          Length = 981

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TSKLQSFE 9
           T K+QSFE
Sbjct: 765 TIKMQSFE 772
>NP_006337
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SKLQSFE 9
           SKL+SFE
Sbjct: 460 SKLKSFE 466
>NP_056496
          Length = 204

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TSKLQSF 8
           TSKLQ F
Sbjct: 127 TSKLQKF 133
>NP_001106853
          Length = 2093

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KLQSFE 9
            KLQSFE
Sbjct: 1666 KLQSFE 1671
>NP_065928
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    ITSKLQSFE 9
            I  KLQ FE
Sbjct: 2570 INQKLQDFE 2578
>XP_005256667
          Length = 142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TSKLQSF 8
           TSKLQ F
Sbjct: 127 TSKLQKF 133
>XP_005256666
          Length = 153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TSKLQSF 8
           TSKLQ F
Sbjct: 127 TSKLQKF 133
>XP_005256665
          Length = 155

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TSKLQSF 8
           TSKLQ F
Sbjct: 127 TSKLQKF 133
>XP_005256664
          Length = 161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TSKLQSF 8
           TSKLQ F
Sbjct: 127 TSKLQKF 133
>XP_005256662
          Length = 186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TSKLQSF 8
           TSKLQ F
Sbjct: 127 TSKLQKF 133
>XP_005256527
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    ITSKLQSFE 9
            I  KLQ FE
Sbjct: 2570 INQKLQDFE 2578
>XP_006719819
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  SKLQSFE 9
          SKL+SFE
Sbjct: 35 SKLKSFE 41
>XP_006712889
          Length = 2069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    KLQSFE 9
            KLQSFE
Sbjct: 1651 KLQSFE 1656
>NP_892006
          Length = 8797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7449 SKLQSF 7454
>NP_149062
          Length = 8749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7378 SKLQSF 7383
>XP_006715488
          Length = 8721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
>XP_006715487
          Length = 8744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
>XP_006715486
          Length = 8758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
>XP_006715485
          Length = 8793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7408 SKLQSF 7413
>XP_006715484
          Length = 8794

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7409 SKLQSF 7414
>XP_006715483
          Length = 8795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7410 SKLQSF 7415
>XP_006715482
          Length = 8797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7449 SKLQSF 7454
>XP_006715481
          Length = 8799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7414 SKLQSF 7419
>XP_006715480
          Length = 8799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7414 SKLQSF 7419
>XP_006715479
          Length = 8804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7456 SKLQSF 7461
>XP_006715478
          Length = 8809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
>XP_006715477
          Length = 8822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7437 SKLQSF 7442
>XP_006715476
          Length = 8823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
>XP_006715475
          Length = 8827

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7456 SKLQSF 7461
>XP_006715474
          Length = 8829

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7444 SKLQSF 7449
>XP_006715473
          Length = 8832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
>XP_006715472
          Length = 8839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7454 SKLQSF 7459
>XP_006715471
          Length = 8842

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7457 SKLQSF 7462
>XP_006715470
          Length = 8846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SKLQSF 8
            SKLQSF
Sbjct: 7461 SKLQSF 7466
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.122    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 354,222
Number of extensions: 601
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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