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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001265484                                                           17    4312
NP_001265483                                                           17    4312
NP_872619                                                              17    4312
NP_006348                                                              17    4312
XP_005246301                                                           17    4312
NP_443099                                                              16    5631
NP_072053                                                              16    5631
NP_000506                                                              16    5631
NP_001308                                                              16    5631
NP_072050                                                              16    5631
NP_002050                                                              16    5631
NP_072052                                                              16    5631
NP_072051                                                              16    5631
XP_005257276                                                           16    5631
XP_005257275                                                           16    5631
XP_006720008                                                           16    5631
XP_006720007                                                           16    5631
XP_006720006                                                           16    5631
NP_001309                                                              16    5631
NP_072101                                                              16    5631
NP_072103                                                              16    5631
NP_072102                                                              16    5631
NP_072170                                                              16    5631
NP_066271                                                              16    5631
NP_001139150                                                           16    7355
NP_666016                                                              16    7355
XP_006714651                                                           16    7355
NP_848622                                                              15    9605
NP_997299                                                              15    9605
NP_001138669                                                           15    9605
NP_619646                                                              15    9605
XP_005276416                                                           15    9605
XP_006716233                                                           15    9605
XP_005265371                                                           15    9605
NP_443085                                                              15    9605

>NP_001265484
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TQQKSNT 8
          TQQK NT
Sbjct: 43 TQQKKNT 49



>NP_001265483
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TQQKSNT 8
          TQQK NT
Sbjct: 43 TQQKKNT 49



>NP_872619
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TQQKSNT 8
          TQQK NT
Sbjct: 43 TQQKKNT 49



>NP_006348
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TQQKSNT 8
          TQQK NT
Sbjct: 43 TQQKKNT 49



>XP_005246301
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  TQQKSNT 8
          TQQK NT
Sbjct: 43 TQQKKNT 49



>NP_443099
          Length = 1738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITQQKSNTV 9
           +TQ  SNTV
Sbjct: 785 LTQDHSNTV 793



>NP_072053
          Length = 202

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 78 TQQKSN 83



>NP_000506
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>NP_001308
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>NP_072050
          Length = 202

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 78 TQQKSN 83



>NP_002050
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>NP_072052
          Length = 245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>NP_072051
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>XP_005257276
          Length = 171

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>XP_005257275
          Length = 179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>XP_006720008
          Length = 1645

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITQQKSNTV 9
           +TQ  SNTV
Sbjct: 692 LTQDHSNTV 700



>XP_006720007
          Length = 1709

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITQQKSNTV 9
           +TQ  SNTV
Sbjct: 756 LTQDHSNTV 764



>XP_006720006
          Length = 1713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITQQKSNTV 9
           +TQ  SNTV
Sbjct: 760 LTQDHSNTV 768



>NP_001309
          Length = 128

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2 TQQKSN 7
         TQQKSN
Sbjct: 4 TQQKSN 9



>NP_072101
          Length = 222

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TQQKSN 7
           TQQKSN
Sbjct: 98  TQQKSN 103



>NP_072103
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 75 TQQKSN 80



>NP_072102
          Length = 139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 15 TQQKSN 20



>NP_072170
          Length = 167

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>NP_066271
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TQQKSN 7
          TQQKSN
Sbjct: 93 TQQKSN 98



>NP_001139150
          Length = 1275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ITQQKSNTV 9
            +T+QK NT+
Sbjct: 1119 LTEQKINTM 1127



>NP_666016
          Length = 643

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ITQQKSNTV 9
           I ++KSNT+
Sbjct: 98  IKKEKSNTI 106



>XP_006714651
          Length = 1280

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ITQQKSNTV 9
            +T+QK NT+
Sbjct: 1124 LTEQKINTM 1132



>NP_848622
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ITQQKSNT 8
            +TQ+ SNT
Sbjct: 1093 LTQRNSNT 1100



>NP_997299
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ITQQKSN 7
           +TQ KSN
Sbjct: 308 VTQSKSN 314



>NP_001138669
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ITQQKSN 7
           +TQ KSN
Sbjct: 308 VTQSKSN 314



>NP_619646
          Length = 179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ITQQKSNT 8
           I+QQ SNT
Sbjct: 160 ISQQYSNT 167



>XP_005276416
          Length = 1583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ITQQKSN 7
           +TQ KSN
Sbjct: 297 VTQSKSN 303



>XP_006716233
          Length = 542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   ITQQKSNTV 9
           +TQ + NT+
Sbjct: 466 VTQARKNTI 474



>XP_005265371
          Length = 382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   ITQQKSN 7
           ITQ KSN
Sbjct: 289 ITQTKSN 295



>NP_443085
          Length = 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   ITQQKSNTV 9
           +TQ + NT+
Sbjct: 550 VTQARKNTI 558



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.106    0.243 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 430,971
Number of extensions: 405
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)