Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_055778                                                              17    3301
NP_001093592                                                           17    3301
XP_005259577                                                           17    3301
XP_005259576                                                           17    3301
NP_001180251                                                           16    5631
NP_001180250                                                           16    5631
NP_054721                                                              16    5631
XP_006711704                                                           16    5631
XP_005273413                                                           16    5631
NP_001157882                                                           16    7355
NP_004926                                                              16    7355
XP_006726589                                                           16    7355
XP_006718462                                                           16    7355
XP_006710010                                                           16    7355
NP_705899                                                              15    9605
XP_006713053                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    TLFSTSVD 9
            TLFSTSVD
Sbjct: 1127 TLFSTSVD 1134
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   TLFSTSVD 9
           TLFSTSVD
Sbjct: 922 TLFSTSVD 929
>NP_055778
          Length = 1366

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ITLFSTSVD 9
           I++FS+SVD
Sbjct: 104 ISIFSSSVD 112
>NP_001093592
          Length = 1309

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  ITLFSTSVD 9
          I++FS+SVD
Sbjct: 47 ISIFSSSVD 55
>XP_005259577
          Length = 1366

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ITLFSTSVD 9
           I++FS+SVD
Sbjct: 104 ISIFSSSVD 112
>XP_005259576
          Length = 1366

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   ITLFSTSVD 9
           I++FS+SVD
Sbjct: 104 ISIFSSSVD 112
>NP_001180251
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ITLFSTSV 8
           +TLFST++
Sbjct: 379 LTLFSTAI 386
>NP_001180250
          Length = 631

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ITLFSTSV 8
           +TLFST++
Sbjct: 294 LTLFSTAI 301
>NP_054721
          Length = 716

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ITLFSTSV 8
           +TLFST++
Sbjct: 379 LTLFSTAI 386
>XP_006711704
          Length = 646

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ITLFSTSV 8
           +TLFST++
Sbjct: 379 LTLFSTAI 386
>XP_005273413
          Length = 716

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ITLFSTSV 8
           +TLFST++
Sbjct: 379 LTLFSTAI 386
>NP_001157882
          Length = 260

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LFSTSVD 9
           L+STS+D
Sbjct: 146 LYSTSID 152
>NP_004926
          Length = 292

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LFSTSVD 9
           L+STS+D
Sbjct: 178 LYSTSID 184
>XP_006726589
          Length = 931

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ITLFSTS 7
           +TLFSTS
Sbjct: 537 MTLFSTS 543
>XP_006718462
          Length = 4966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    ITLFSTS 7
            +TLFSTS
Sbjct: 4572 MTLFSTS 4578
>XP_006710010
          Length = 1103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ITLFSTS 7
           +TLFSTS
Sbjct: 709 MTLFSTS 715
>NP_705899
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITLFSTSVD 9
           +TL S SVD
Sbjct: 275 VTLLSFSVD 283
>XP_006713053
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITLFSTSVD 9
           +TL S SVD
Sbjct: 275 VTLLSFSVD 283
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.131    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 338,942
Number of extensions: 496
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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