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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              20     509
NP_009297                                                              20     509
NP_001885                                                              17    2528
NP_689407                                                              17    2528
NP_001276841                                                           17    2528
NP_001884                                                              17    2528
NP_620693                                                              17    2528
XP_005256394                                                           17    2528
XP_005256393                                                           17    2528
NP_002652                                                              17    4312
NP_002527                                                              17    4312
NP_060889                                                              17    4312
NP_073153                                                              16    5631
NP_055496                                                              16    5631
NP_005611                                                              16    5631
XP_005259500                                                           16    5631
XP_006712754                                                           16    5631
XP_006712753                                                           16    5631
NP_001161710                                                           16    7355
NP_001161709                                                           16    7355
NP_001161708                                                           16    7355
NP_009298                                                              16    7355
NP_001129473                                                           16    7355
NP_922932                                                              16    7355
NP_005149                                                              16    7355
NP_001161711                                                           16    7355
XP_005245145                                                           16    7355
NP_001129472                                                           16    7355
NP_996816                                                              15    9605
NP_005443                                                              15    9605
NP_001157739                                                           15    9605
XP_005275548                                                           15    9605
XP_005275377                                                           15    9605
XP_005275121                                                           15    9605
XP_005272965                                                           15    9605
XP_005249538                                                           15    9605

>NP_005148
          Length = 1130

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ITEFMTYLN 9
           ITEFMTY N
Sbjct: 314 ITEFMTYGN 322



>NP_009297
          Length = 1149

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ITEFMTYLN 9
           ITEFMTY N
Sbjct: 333 ITEFMTYGN 341



>NP_001885
          Length = 416

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFSTYLN 253



>NP_689407
          Length = 416

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFSTYLN 253



>NP_001276841
          Length = 416

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFSTYLN 253



>NP_001884
          Length = 415

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFATYLN 253



>NP_620693
          Length = 409

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFATYLN 253



>XP_005256394
          Length = 427

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFATYLN 253



>XP_005256393
          Length = 450

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           +EF TYLN
Sbjct: 246 SEFATYLN 253



>NP_002652
          Length = 1265

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ITEFMTYL 8
           + EF+TYL
Sbjct: 287 VDEFLTYL 294



>NP_002527
          Length = 688

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EFMTYLN 9
           EF TYLN
Sbjct: 203 EFHTYLN 209



>NP_060889
          Length = 384

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TEF  YLN
Sbjct: 324 LTEFDQYLN 332



>NP_073153
          Length = 1944

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    EFMTYLN 9
            +FMTYL+
Sbjct: 1494 DFMTYLS 1500



>NP_055496
          Length = 1143

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    ITEFMTY 7
            +T+F+TY
Sbjct: 1043 VTDFLTY 1049



>NP_005611
          Length = 105

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  TEFMTYLN 9
          TEF++++N
Sbjct: 37 TEFLSFMN 44



>XP_005259500
          Length = 1143

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    ITEFMTY 7
            +T+F+TY
Sbjct: 1043 VTDFLTY 1049



>XP_006712754
          Length = 1784

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    EFMTYLN 9
            +FMTYL+
Sbjct: 1334 DFMTYLS 1340



>XP_006712753
          Length = 1846

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    EFMTYLN 9
            +FMTYL+
Sbjct: 1396 DFMTYLS 1402



>NP_001161710
          Length = 1058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 339 VTEYMPYGN 347



>NP_001161709
          Length = 1079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 360 VTEYMPYGN 368



>NP_001161708
          Length = 1161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 339 VTEYMPYGN 347



>NP_009298
          Length = 1182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 360 VTEYMPYGN 368



>NP_001129473
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 339 VTEYMPYGN 347



>NP_922932
          Length = 1117

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  EFMTYL 8
          EF+TYL
Sbjct: 34 EFLTYL 39



>NP_005149
          Length = 1167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 345 VTEYMPYGN 353



>NP_001161711
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 324 VTEYMPYGN 332



>XP_005245145
          Length = 1146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 324 VTEYMPYGN 332



>NP_001129472
          Length = 1064

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ITEFMTYLN 9
           +TE+M Y N
Sbjct: 345 VTEYMPYGN 353



>NP_996816
          Length = 5202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1    ITEFMTYLN 9
            +TE+  Y+N
Sbjct: 3050 VTEYSIYVN 3058



>NP_005443
          Length = 610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 296 TGFLTYLD 303



>NP_001157739
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 242 TGFLTYLD 249



>XP_005275548
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 296 TGFLTYLD 303



>XP_005275377
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 296 TGFLTYLD 303



>XP_005275121
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 296 TGFLTYLD 303



>XP_005272965
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 296 TGFLTYLD 303



>XP_005249538
          Length = 754

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TEFMTYLN 9
           T F+TYL+
Sbjct: 296 TGFLTYLD 303



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.140    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 443,863
Number of extensions: 741
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)